FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543569
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC59870.387867338615896No Hit
GCCCTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTG52650.34109262365336435No Hit
GGTTAACAGTGATGGCAGTCAATACAAGGGGGGCGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTCA50950.3300791866123251No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTGAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG48220.31239290242289136No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT46890.30377650756137237No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45700.2960671016326449No Hit
ATACAAGGGGGGCGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTCACCGCTTCCTCACCATCTCCAG42140.27300366877023313No Hit
TCCTAGGTCAGCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41100.2662660366980679No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38170.24728405403321782No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36260.2349101335929913No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC34360.22260099807653563No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33490.2169647097084743No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG32940.21340153890107927No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32710.21191148565435042No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31970.20711740129530976No Hit
CTGTTAACCTGCATCAAGAACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGGTGCTGTGG31770.20582170281989337No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31630.20491471388710192No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31290.20271202647889405No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31040.20109240338462356No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG30580.19811229689116586No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT30550.19791794211985342No Hit
GTCCTAGGTCAGCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC29230.18936633218210522No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28900.1872284296976682No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCACTCAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAG28010.18146257148206527No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27050.1752432188000666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26710.17304053139185874No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26620.17245746707792137No Hit
GTAGTATGCGGCCGTGTCGTCGGCTCTCAGGCTGCTCATTTGCAGATACA26570.17213354245906726No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26310.17044913444102597No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCCTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC26090.16902386611806794No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGAAGC25940.16805209226150564No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25270.16371150236886076No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24160.15652037583029976No Hit
CCTGCACTCTGAGCAGTGGCCACAGCACCTACGCCATCGCATGGCATCAG24080.15600209644013324No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23080.1495236040630513No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC23050.14932924929173882No Hit
CCCTTGTATTGACTGCCATCACTGTTAACCTGCATCAAGAACCGAGGGCC22430.14531258401794803No Hit
CACCTGCACTCTGAGCAGTGGCCACAGCACCTACGCCATCGCATGGCATC22260.14421124031384408No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22210.14388731569499No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCCTGGG22000.1425268322958028No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT21710.14064806950644904No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG21230.1375383931654497No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21090.1366314042326582No Hit
CTATTACTGTCAGACCTGGGGCACTGGCACTCAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCC20650.13378086758674215No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20580.13332737312034643No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20340.13177253494984675No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTGAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC20060.12995855708426382No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCCTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC19930.12911635307524316No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19910.12898678322770152No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19820.12840371891376412No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19660.12736716013343102No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19660.12736716013343102No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19510.12639538627686875No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19340.1252940425727648No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19060.12348006470718186No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACTC19020.1232209250120986No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18840.12205479638422384No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18680.12101823760389072No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGAAGCGGTGAGCCCCAGAGCTGGAGC18510.1199168938997868No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18430.11939861450962024No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17750.11499323969320452No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17720.11479888492189207No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGAAGCGGTGAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC17610.11408625076041305No Hit
GCTCTGGGGCTCACCGCTTCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC17440.11298490705630912No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17310.11214270304728846No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT17160.11117092919072616No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15650.10138840570133242No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15630.1012588358537908No Hit
CCTGAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCA15570.10087012631116589No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15520.10054620169231178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)