FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437927
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA25310.5779502063129243No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGGTAATACCTGCATATGAGCAG20080.45852390923601427No Hit
GATTATTACTGCTGCTCATATGCAGGTATTACCACTTTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT18680.42655511078330405No Hit
GTAATAATCAGCGTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG17840.40737383171167796No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGGTAATACCTGCATATGAGCAGCAGTAATAAT17820.406917134590925No Hit
GTATATTACTGTGCGAGACATGACTTAATACTGGGAGCTACTTCTCCAAATTTTGACTACTGGGGCCAGGGAACC16720.38179879294950986No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT16380.37403494189670883No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT15920.36353090811938976No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15330.3500583430571762No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14440.3297353211836676No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13440.3069004651460175No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13220.30187679681773444No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACTTTGTCTCCTGGTTCCAACAACA13060.2982232198517104No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACTTTGTCTCCTGGTTCCAACAACACCCAGGCAATGC12440.28406560910836737No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11940.2726481810895423No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11940.2726481810895423No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG11820.26990799836502427No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTATTTCCTACTGGGGCTGGATCCG11690.2669394670801298No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11450.26145910163109376No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11370.2596323131480818No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10100.23063204598026613No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10000.22834856037650111No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9320.21282085827089903No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9260.21145076690864004No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8890.20300187017470947No Hit
GTATTAAGTCATGTCTCGCACAGTAATATACAGCCGTGTCTGCGGCGGTC8880.20277352161433299No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8790.20071838457094449No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8750.19980499032943846No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8620.19683645904454397No Hit
GAGTTATAACTTTGTCTCCTGGTTCCAACAACACCCAGGCAATGCCCCCA8500.19409627632002593No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8500.19409627632002593No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATCATGAGTTTGGGGGCATTGCCTGGGTGTTGTTGGAACCAGGAGA8410.19204113927663743No Hit
CTATTATAGTGGGAACAGCTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTCGACACGTCCAAGAA8220.1877025166294839No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8090.1847339853445894No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7850.17925361989555336No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGGTAATAC7670.17514334580877633No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7440.16989132892011682No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTATTTCCTACTGGG7290.1664661005144693No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7250.1655527062729633No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7250.1655527062729633No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7240.1653243577125868No Hit
GTCAATAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT7060.1612140836258098No Hit
CACCCAGGCAATGCCCCCAAACTCATGATTTATGAGGTCAATAAGCGGCCCTCAGGGGTTTCTAATCGCTTCT6830.15596206673715024No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6660.15208014121074975No Hit
GGTAATACCTGCATATGAGCAGCAGTAATAATCAGCGTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGC6620.15116674696924373No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG6590.15048170128811422No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6560.1497966556069847No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6490.1481982156843492No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6490.1481982156843492No Hit
GTGGTAATACCTGCATATGAGCAGCAGTAATAATCAGCGTCGTCCTCAGC6480.14796986712397273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.14363124447681921No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6280.14340289591644267No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT6130.13997766751079516No Hit
CTTATTGACCTCATAAATCATGAGTTTGGGGGCATTGCCTGGGTGTTGTTGGAACCAGGAGACAAAGTTATAACT6090.13906427326928916No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC6090.13906427326928916No Hit
GCTTGGTCCCTCCGCCGAAGACCAAAGTGGTAATACCTGCATATGAGCAGCAGTAATAATCAGCGTCGTCCTCAG6070.1386075761485362No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6030.13769418190703017No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC6000.13700913622590066No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG5930.13541069630326516No Hit
ACTTAATACTGGGAGCTACTTCTCCAAATTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCAT5900.13472565062213565No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC5880.13426895350138265No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC5830.13312721069950015No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5810.13267051357874715No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG5600.12787519381084061No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC5590.12764684525046413No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5540.1265051024485816No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5510.1258200567674521No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5490.12536335964669912No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC5450.1244499654051931No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA5400.1233082226033106No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT5400.1233082226033106No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5370.1226231769221811No Hit
GTAGTAGCTGTTCCCACTATAATAGATACTCCCAATCCACTCGAGTCCCTTCCCTGGGGGCTGGCGGATCCAGCC5300.12102473699954559No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC5280.12056803987879258No Hit
CTATAATAGATACTCCCAATCCACTCGAGTCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGGAAATACTA5260.12011134275803957No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5250.11988299419766309No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC5230.11942629707691008No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5140.11737116003352156No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC4990.11394593162787406No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG4980.11371758306749755No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4900.11189079458448555No Hit
GGCAATGCCCCCAAACTCATGATTTATGAGGTCAATAAGCGGCCCTCAGG4840.11052070322222654No Hit
ATCTATTATAGTGGGAACAGCTACTACAGTCCGTCCCTCAAGAGTCGAGT4830.11029235466185003No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4820.11006400610147353No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4780.10915061185996754No Hit
CAACAACACCCAGGCAATGCCCCCAAACTCATGATTTATGAGGTCAATAAGCGGCCCTCAGGGGTTTCTAATCG4770.10892226329959102No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC4710.10755217193733203No Hit
GTATTACCACTTTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA4690.10709547481657902No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC4680.10686712625620252No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC4630.10572538345432002No Hit
GTTATAACTTTGTCTCCTGGTTCCAACAACACCCAGGCAATGCCCCCAAA4550.10389859497130799No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG4520.1032135492901785No Hit
GTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG4500.10275685216942551No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT4480.1023001550486725No Hit
GATATGGAGACTCGACTCTTGAGGGACGGACTGTAGTAGCTGTTCCCACT4420.10093006368641348No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAATTTGGAGAAGTAGCTCCCAGTATTAAGTCATGTCTCGCACAGTAATATA4420.10093006368641348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)