FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437927
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30820.7037702630803764No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA23680.5407293909715547No Hit
GATTATTACTGCTGCTCATATGCAGGTATTACCACTTTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT21640.49414628465474836No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGGTAATACCTGCATATGAGCAG17320.3954997065720999No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15750.3596489825929892No Hit
GTATATTACTGTGCGAGACATGACTTAATACTGGGAGCTACTTCTCCAAATTTTGACTACTGGGGCCAGGGAACC15370.3509717372986822No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15370.3509717372986822No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGGTAATACCTGCATATGAGCAGCAGTAATAAT14420.3292786240629146No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG14300.3265384413383966No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACTTTGTCTCCTGGTTCCAACAACACCCAGGCAATGC14200.3242549557346316No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13660.3119241334743005No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13520.3087272536290295No Hit
GTAATAATCAGCGTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG13490.3080422079479No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12040.2749316666933073No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACTTTGTCTCCTGGTTCCAACAACA11870.27104974116690683No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11680.26671111851975327No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTATTTCCTACTGGGGCTGGATCCG11380.25986066170845823No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11100.2534669020179162No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11040.25209681065565726No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9970.2276635146953716No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9510.21715948091805254No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9440.21556104099541706No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9080.207340492821863No Hit
GTATTAAGTCATGTCTCGCACAGTAATATACAGCCGTGTCTGCGGCGGTC8500.19409627632002593No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8330.19021435079362542No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8290.1893009565521194No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8220.1877025166294839No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATCATGAGTTTGGGGGCATTGCCTGGGTGTTGTTGGAACCAGGAGA8120.1854190310257189No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7890.18016701413705935No Hit
CTATTATAGTGGGAACAGCTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTCGACACGTCCAAGAA7870.17971031701630638No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC7780.17765517997291785No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7770.17742683141254137No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7680.17537169436915287No Hit
GAGTTATAACTTTGTCTCCTGGTTCCAACAACACCCAGGCAATGCCCCCA7660.17491499724839984No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTATTTCCTACTGGG7580.17308820876538783No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7530.17194646596350532No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG7360.1680645404371048No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7250.1655527062729633No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7230.1650960091522103No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7190.1641826149107043No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG7140.1630408721088218No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6950.15870224946166828No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6880.15710380953903277No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6740.15390692969376174No Hit
GTCAATAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT6650.15185179265037324No Hit
GTGGTAATACCTGCATATGAGCAGCAGTAATAATCAGCGTCGTCCTCAGC6650.15185179265037324No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6610.15093839840886722No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC6380.14568638152020771No Hit
CACCCAGGCAATGCCCCCAAACTCATGATTTATGAGGTCAATAAGCGGCCCTCAGGGGTTTCTAATCGCTTCT6240.14248950167493668No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC6180.1411194103126777No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAAAGTGGTAATAC6110.1395209703900422No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC5900.13472565062213565No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG5890.13449730206175917No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5870.13404060494100614No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT5830.13312721069950015No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5660.12924528517309963No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT5530.1262767538882051No Hit
GCTTGGTCCCTCCGCCGAAGACCAAAGTGGTAATACCTGCATATGAGCAGCAGTAATAATCAGCGTCGTCCTCAG5460.12467831396556961No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG5450.1244499654051931No Hit
GGTAATACCTGCATATGAGCAGCAGTAATAATCAGCGTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGC5440.1242216168448166No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5400.1233082226033106No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT5370.1226231769221811No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC5350.12216647980142809No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA5350.12216647980142809No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5350.12216647980142809No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5350.12216647980142809No Hit
TCTCTGGGCTCCAGGCTGAGGACGACGCTGATTATTACTGCTGCTCATAT5320.12148143412029858No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC5220.11919794851653358No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5180.11828455427502758No Hit
ACTTAATACTGGGAGCTACTTCTCCAAATTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCAT5160.11782785715427457No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5160.11782785715427457No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5110.11668611435239205No Hit
CTTATTGACCTCATAAATCATGAGTTTGGGGGCATTGCCTGGGTGTTGTTGGAACCAGGAGACAAAGTTATAACT5070.11577272011088606No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5070.11577272011088606No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA4920.11234749170523854No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT4760.10869391473921453No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC4710.10755217193733203No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4710.10755217193733203No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAATTTGGAGAAGTAGCTCCCAGTATTAAGTCATGTCTCGCACAGTAATATA4610.10526868633356701No Hit
CAACAACACCCAGGCAATGCCCCCAAACTCATGATTTATGAGGTCAATAAGCGGCCCTCAGGGGTTTCTAATCG4550.10389859497130799No Hit
GTTATAACTTTGTCTCCTGGTTCCAACAACACCCAGGCAATGCCCCCAAA4530.103441897850555No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG4500.10275685216942551No Hit
GTATTACCACTTTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA4400.10047336656566047No Hit
CCCTGACAATCTCTGGGCTCCAGGCTGAGGACGACGCTGATTATTACTGC4390.10024501800528399No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC4380.10001666944490749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)