FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733733
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29240.3985100847310943No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22120.3014720613629208No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACTTGATCACTACTACTATCCCACAC22010.29997287841762604No Hit
ATATAGAAGTGTGCACTGGTACCACCAGAGGCCAGGCCAGGCCCCTGTGC20190.27516821514093004No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19970.2721698492503404No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19650.2678085897731191No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19090.2601763856879819No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18590.2533619177548236No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG18580.2532256283961605No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACTTGATCACTA18520.2524078922441815No Hit
CTTCTATATCCAATCCTGTTTGCCCCACAGGTGATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG18290.24927323699492865No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17100.23305480331401204No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15740.2145194505358216No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15640.21315655694918995No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15520.21152108464523198No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14990.20429774863608424No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14980.2041614592774211No Hit
CTACTACTATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTC14680.20007277851752614No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13780.18780673623784128No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13700.18671642136853597No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT13570.18494465970591484No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13240.18044711087003037No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13090.1784027704900829No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13060.1779939024140934No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12980.17690358754478808No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12850.17513182588216694No Hit
CGGCCACCCTGACCATTAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT12640.17226974935024048No Hit
TAGTAGTAGTGATCAAGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC12620.17199717063291414No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12600.1717245919155878No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12340.16818106859034554No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG12180.1660004388517349No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11960.16300207296114527No Hit
GGGTGGCCGTGTCCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA11780.16054886450520828No Hit
CCCCAGGACAGACGGCCAGGATCACCTGTGGGGCAAACAGGATTGGATAT11720.1597311283532293No Hit
GGATAGTAGTAGTGATCAAGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC11500.15673276246263967No Hit
CTACTATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGAC11250.15332552849606054No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11040.1504634519641341No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10740.14637477120423914No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10570.14405785210696534No Hit
CTATTATTGTCAGGTGTGGGATAGTAGTAGTGATCAAGTGGTATTCGGCG10380.1414683542923652No Hit
GCACAGGGGCCTGGCCTGGCCTCTGGTGGTACCAGTGCACACTTCTATAT10220.13928772455375457No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9930.1353353331525228No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT9920.1351990437938596No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9920.1351990437938596No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9700.13220067790326998No Hit
GGATTGGATATAGAAGTGTGCACTGGTACCACCAGAGGCCAGGCCAGGCC9620.13111036303396467No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC9540.13002004816465934No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9440.1286571545780277No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATCAC9390.12797570778471187No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGGACACGG9330.1271579716327329No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9250.12606765676342757No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9080.12375073766615376No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9080.12375073766615376No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA9020.12293300151417477No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8690.11843545267829034No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC8670.11816287396096399No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG8620.11748142716764819No Hit
GTAGTAGTGATCAAGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA8600.11720884845032185No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8500.11584595486369019No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8440.1150282187117112No Hit
GGATATAGAAGTGTGCACTGGTACCACCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGAC8370.11407419320106904No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8360.11393790384240589No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT8300.1131201676904269No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8290.11298387833176372No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACTTGATCACTAC8270.1127112996144374No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8260.11257501025577425No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC8010.1091677762891951No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA7850.10698714655058449No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7850.10698714655058449No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7730.10535167424662649No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT7670.1045339380946475No Hit
CAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTAATGGTCAGGGT7610.10371620194266852No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT7570.10317104450801587No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7520.10248959771470004No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG7510.10235330835603687No Hit
GGTACCACCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCC7440.10139928284539472No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA7410.1009904147694052No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7380.10058154669341574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1550.044.41038538
ATGCCGT1900.043.8104644
TCGTATG1750.043.37898340
TATGCCG1850.043.06593743
GGTATCA6200.042.851391
TCGACGT2000.042.6202929
CGTATGC1800.042.21483241
GTATCAA18700.041.7115061
CTTATAC2250.040.873641
CTCGACG2100.040.5713728
GACTCGA2150.039.60623626
ATCTCGT1750.039.26188737
GACGTCA2300.038.59097731
CGACGTC2300.038.56462530
ACGAGAC2300.038.49102422
CCCACGA2300.038.48578319
ACTCGAC2250.037.85886827
TCCGAGC2350.037.66950213
AGACTCG2300.037.01817325
TATCAAC21750.036.1631852
Produced by FastQC (version 0.11.5)