FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524175
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAAGGTGCTGATGGTGAGAGTGAAATCTGTC328012.1520494693850774No Hit
GTATTATACTGCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT319232.0944445355684222No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT123880.8127675627798645No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC88810.5826758738333853No Hit
CTTTTATACACCTCCAACAATAAGAACTACTTAGCTTGGTACCAACAAAG85100.5583348368789673No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC77870.510899338986665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC77410.5078813128413732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT68150.4471271343513704No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTAAATCG64740.4247543753177949No Hit
ACTTAGGAGGTTGTCCTGGTCTTTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGTGTATAAAAGACT63630.41747174701067785No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG61980.4066462184460446No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC57350.37626912920104316No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC56890.3732511030557515No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG55410.3635409319795955No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA54170.3554053832401135No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA53520.3511407810782882No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT53160.3487788475732773No Hit
CTCCTAAGTTGCTCGTTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCT53090.3483195827250808No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT51940.3407745173618515No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG50340.33027703511735856No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49860.3271277904440107No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT49510.3248314662030279No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG47480.31151278560532747No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG47010.30842915019600764No Hit
GTTTATTACTGTCAGCAGTATTATACTGCTCCGACGTTCGGCCAAGGGAC46910.30777305755572687No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC46140.3027211442255646No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAAGGTGCTGATGGTGAGAGTGA45410.2979316679515147No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA43270.2838912854495055No Hit
GTCCCAGACCCGCTGCCACTAAATCGGTCAGGGACCCCGGATTCCCGGGT43160.28316958354519656No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT42380.2780520609510063No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38360.2516771368117178No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG37210.24413207144848853No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36910.2421637935276461No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG36900.242098184263618No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACACCTCCAA36480.23934259517443862No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA36470.23927698591041055No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTAAATCGGTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGGAAA36440.2390801581183263No Hit
GACCAGGACAACCTCCTAAGTTGCTCGTTTCCTGGGCGTCTACCCGGGAA36080.2367182246133154No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC36060.23658700608525923No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC35760.2346187281644168No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA34030.22326832548755887No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA33830.22195614020699725No Hit
CCATCAGCACCTTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAG33280.21834763068545282No Hit
CCTCCTAAGTTGCTCGTTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCTGACCGATTTAGTGGCAGCGGGT32760.2149359489559926No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC32320.21204914133875702No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT31270.20516016861580855No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA30800.20207653320648877No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA30660.20115800351009563No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29950.1964997457641019No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29900.19617169944396148No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT29660.19459707710728755No Hit
GATTTCACTCTCACCATCAGCACCTTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATACTGCT29390.19282562697852937No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC29250.19190709728213626No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT28830.18915150819295684No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC28830.18915150819295684No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC28430.18652713763183362No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC28310.18573982646349663No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC27850.18272180031820492No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG27740.18200009841389606No Hit
TCTTCAGCCTGCAAGGTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTAAATCGGTCAGGGACC27560.18081913166139058No Hit
GTACCAACAAAGACCAGGACAACCTCCTAAGTTGCTCGTTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCTGA27250.17878524447652008No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACACCTCCAACAATAAGAACTA27090.1777354962520708No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA26470.1736677218823298No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA26040.1708465235291223No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT25580.1678284973838306No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC25550.16763166959174636No Hit
ATACTGCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT25480.1671724047435498No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25440.1669099676874375No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC25350.16631948431118476No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT25140.16494168976659504No Hit
CTACGGGGACATCGTGATGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG25110.1647448619745108No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT25060.16441681565437039No Hit
GTCCCTGACCGATTTAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC24930.16356389522200535No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT24910.1634326766939492No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC24890.16330145816589303No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA24780.16257975626158414No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24270.15923368379615202No Hit
CCTCCAACAATAAGAACTACTTAGCTTGGTACCAACAAAGACCAGGACAACCTCCTAAGTTGCTCGTTTCCTGGG24140.15838076336378695No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA23940.15706857808322536No Hit
GTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGGAAACGAGCAACTTAGGAGGTTGTCCTGGTCTTTGTTGGTA23880.15667492249905685No Hit
CTACTTAGCTTGGTACCAACAAAGACCAGGACAACCTCCTAAGTTGCTCGTTTCCTGGGCGTCTACCCGGGAATC23870.15660931323502877No Hit
GTCCTGGTCTTTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGTG23690.15542834648252332No Hit
ATTTAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCACCT23630.15503469089835487No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG23590.15477225384224252No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAAGGTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22980.15077008873652958No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC22960.15063887020847344No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22920.15037643315236113No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT22900.15024521462430496No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCACCTTGCAGGCTGAAGATG22880.1501139960962488No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC22770.1493922941919399No Hit
ACTTAGCTTGGTACCAACAAAGACCAGGACAACCTCCTAAGTTGCTCGTT22490.14755523479915364No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22470.14742401627109747No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC22450.14729279774304133No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21700.14237210294093525No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21570.1415191825085702No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGG20850.1367953154985484No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20810.13653287844243608No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA20730.13600800433021143No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC20640.1354175209539587No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG20490.1344333819935375No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC20200.13253071333672314No Hit
CAATAAGAACTACTTAGCTTGGTACCAACAAAGACCAGGACAACCTCCTAAGTTGCTCGTTTCCTGGGCGTCTAC20070.13167779290435808No Hit
CATCAGCACCTTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGT19980.13108730952810535No Hit
TTATTACTGTCAGCAGTATTATACTGCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG19810.129971952039628No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19680.12911903160726296No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT19420.12741319074253285No Hit
GAGCAGTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAAGGTGCTGATGGTGAGAGTGAAAT19330.12682270736628012No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG19320.12675709810225202No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATACTGCTCCGA19240.1262322239900274No Hit
GAACTACTTAGCTTGGTACCAACAAAGACCAGGACAACCTCCTAAGTTGC19130.1255105220857185No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC19040.12492003870946577No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG18770.1231485885807076No Hit
TTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGTGTATAAAAGAC18710.1227549329965391No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18430.12091787360375285No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17950.11776862893040498No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17880.1173093640822084No Hit
GGACAACCTCCTAAGTTGCTCGTTTCCTGGGCGTCTACCCGGGAATCCGG17790.11671888070595568No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG17720.11625961585775911No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT17660.11586596027359064No Hit
TTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCTGACCGATTTAGTGGCAGCGGGTCTGGGACAGATTTCACT17500.11481621204914134No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGC17440.11442255646497285No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC17230.11304476192038315No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACA17170.11265110633621467No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGCAGTATAATACTGCTGACAGTAA16960.11127331179162497No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT16800.11022356356717568No Hit
GCTTGGTACCAACAAAGACCAGGACAACCTCCTAAGTTGCTCGTTTCCTGGGCGTCTACCCGGGAATCCGGGGTC16700.10956747092689487No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA16620.10904259681467023No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG16510.10832089491036134No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16400.10759919300605246No Hit
ACAAAGACCAGGACAACCTCCTAAGTTGCTCGTTTCCTGGGCGTCTACCC16400.10759919300605246No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGC16400.10759919300605246No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACACCTCCAACAATAA16310.10700870962979973No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG16060.10536847802909771No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16000.10497482244492921No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGGCCCAGTCTCCAG15990.10490921318090113No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15990.10490921318090113No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC15840.10392507422047993No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCACCTTGCAGGCTGAAGAT15800.1036626371643676No Hit
GGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACA15610.10241606114783407No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC15520.10182557777158134No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG15410.10110387586727246No Hit
GTGATGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC15300.10038217396296358No Hit
GTCTTTTATACACCTCCAACAATAAGAACTACTTAGCTTGGTACCAACAAAGACCAGGACAACCTCCTAAGTTG15250.10005412764282316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)