FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023611
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATATCTGGCCGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT57650.5632022320979356No Hit
GTGATAGACGGCCGTGTCGTCGACTGTCAGGCTGTTCATTTGCAGATCAA55840.5455197335706631No Hit
ATATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC38940.380417951741433No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36080.3524776502011018No Hit
GTCTATCACTGTGCGAAAGGGGGTATACTGGTTCGGGCACATATTGTTAA34050.33264589770918834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32620.31867574693902273No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA27490.2685590522180789No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25610.2501927001566025No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23250.22713706671772774No Hit
GGGCTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23050.2251831994771451No Hit
GTATACCCCCTTTCGCACAGTGATAGACGGCCGTGTCGTCGACTGTCAGG22780.2225454787023586No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22350.21834466413510603No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21490.20994303500060083No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21340.20847763457016388No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT21160.2067191540536395No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20890.204081433278853No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20480.20007600543565868No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20440.19968523198754218No Hit
CTCCTGTGCAGCCTCTGGATTTACCTTTAGTGACTTTGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG20380.19909907181536735No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19910.19450748379999824No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19790.1933351634556487No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19690.19235822983535736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19580.19128360285303694No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19480.19030666923274564No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATATCTGG19400.1895251223365126No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT19340.18893896216433784No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19140.18698509492375523No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18780.18346813389070654No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACCTAGCCTGGTA18140.1772157587208422No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18010.1759457450144635No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16840.16451562165705527No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16630.16246406105444355No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT16550.1616825141582105No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16500.16119404734806483No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT16090.1571886195048705No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16090.1571886195048705No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15900.15533244562631704No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15760.1539647385579092No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15650.15289011157558877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15600.15240164476544313No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15370.1501546974387731No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA15320.14966623062862747No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15240.14888468373239444No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15130.147810056750074No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14840.14497694925122923No Hit
CTCCAGGCACACTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14810.14468386916514184No Hit
GGGGTATACTGGTTCGGGCACATATTGTTAACAATTGGTACGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCT14800.14458617580311273No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14680.14341385545876315No Hit
GTTTATTACTGTCAGCAGTATAATATCTGGCCGCCGCTCACTTTCGGCGG14610.14273000192455923No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14480.14145998821818054No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14340.1400922811497727No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14190.13862688071933577No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14080.13755225373701532No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGCGGCCAGATATTATACTG13910.1358914665825201No Hit
CTACGCAGACTCCGTGAAGGGCCGACTCACCATCTCCAGAGACAATTCCAAGAATACGGTTGATCTGCAAATGAA13760.13442606615208316No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13730.13413298606599577No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13700.13383990597990839No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA13600.13286297235961708No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13440.131299878567151No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGTACCAATTGTTAA13360.13051833167091798No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13340.1303229449468597No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13280.12973678477468492No Hit
GGATACCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG12850.12553597020743232No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12760.12465672994917015No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATGGCAAAGTCAC12620.12328902288076232No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12550.1226051693465584No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTTACCTTTAGTGACTTTGCCATGAGTTGGGTCCG12350.12065130210597581No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12220.11938128839959711No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12210.11928359503756798No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12120.11840435477930582No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11910.11635279417669409No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11760.11488739374625713No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11670.11400815348799496No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11660.11391046012596581No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11230.10970964555871321No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11220.10961195219668408No Hit
GTGTTAGCAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCA11220.10961195219668408No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTTACCTTTAGTGACTTTGCC11190.1093188721105967No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT11070.10814655176624714No Hit
GTCTCCAGGCACACTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10970.10716961814595584No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGG10910.10658345797378106No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10790.10541113762943148No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG10740.10492267081928584No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC10700.10453189737116933No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10650.10404343056102368No Hit
GTATGTGCTATAACCACTAGGACTAATAGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT10600.10355496375087801No Hit
GGGTTCCCTGGCCCCAGGAGTCGTACCAATTGTTAACAATATGTGCCCGA10580.10335957702681975No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT10560.1031641903027615No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG10470.10228495004449933No Hit
GATATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT10370.10130801642420802No Hit
TCTATGATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10360.10121032306217889No Hit
GTCAGAGTGTTAGCAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG10340.10101493633812063No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10320.10081954961406238No Hit
AACCAGTATACCCCCTTTCGCACAGTGATAGACGGCCGTGTCGTCGACTGTCAGGCTGTTCATTTGCAGATCAAC10300.10062416289000412No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10280.10042877616594585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT309.118983E-657.50665739
GTAACCA350.00534549856.10177670
CGAAAAC200.00761836551.29823723
TCGTAAG200.007627199451.2832033
CGTAAGT200.007627199451.2832034
TCTGGAG15100.049.84760370
GGTATCA6550.046.477721
TCGTATG1750.043.4171240
CGTATGC1700.042.7025841
CTTATAC2100.040.7208481
TAGCGCT1950.040.40222529
CTAGCGC1950.040.38443828
GTATCAA17450.039.5960661
TGTCGTA451.0051663E-438.3094438
ATCTCGT1450.038.00966337
GTGGTAT1350.038.0061261
TATACAC2350.037.8259123
CGCTCAT2100.037.5586332
CCGTCTT1950.037.44799847
TACACAT2100.037.444885
Produced by FastQC (version 0.11.5)