FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459608
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11000.2393343893056692No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC9560.20800334197838155No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9370.20386938434492No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8960.1949487389253451No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT8800.19146751144453533No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8740.19016205113923168No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT8240.1792832152617013No Hit
GTCCCAGACCCACTGCCACTGAATTTGGCTGGGATGCCAGTGGCCCTGTT8230.17906563854415067No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7800.1697098396894745No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7280.15839585037684287No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7130.15513219961358374No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6880.14969278167481856No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA6870.14947520495726793No Hit
GTTTATTACTGTCAACAGCGTAATGACTGGCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA6850.14904005152216673No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6710.1459939774764582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6620.14403578701850273No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6430.13990182938504117No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA6420.13968425266749057No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG6390.13903152251483875No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTAATGACTGGCCTCCCACTTTCGGCGGAGGGA6360.13837879236218692No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6040.13141633740056743No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5970.12989330037771318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5920.12880541678996013No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT5870.1277175332022071No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5830.12684722633200465No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5790.12597691946180223No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC5670.12336599885119494No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5530.12031992480548641No Hit
GCGTAATGACTGGCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT5480.11923204121773337No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG5410.11770900419487912No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT5400.1174914274773285No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5330.11596839045447424No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA5300.11531566030182241No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGCCAGGGGCTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT5270.11466293014917059No Hit
GGCTAAGCCCCTGGCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTC5150.1120520095385633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5110.11118170266836086No Hit
GTCTCCAGCCACCTTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT5050.10987624236305721No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC4920.10704774503489932No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4910.10683016831734869No Hit
GTCATTACGCTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT4880.10617743816469687No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC4850.10552470801204504No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4800.10443682442429202No Hit
GGCTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATTCAACAGGGCCA4770.10378409427164019No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTAATGACTGG4760.10356651755408956No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4640.10095559694348226No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4620.10052044350838106No Hit
TCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAG4600.10008529007327985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTA150.001769488374.1517557
AAACGTC150.002104257270.9831746
AAGCCGG350.003642515361.7956670
CAATCTC200.00562740955.37547356
TATGGCT301.229806E-554.6672864
CGTGAGA200.00601032254.46188452
GAAACCT200.00649685953.40081447
TCCCTAT303.0688997E-453.2311167
TCTTATG200.007388807451.6917939
ATTCCTA250.00817588950.3831268
GACAGTT304.421971E-449.43449857
TCCTAAG450.00984404748.06328670
GTGTAAT308.068033E-443.757631
CTTATAC805.820766E-1141.0227781
GGTGTGC350.001726491737.4861378
GTATGGC350.001726491737.4861373
TGTTCGC350.001726491737.48613710
GGTATCA14800.037.474861
CAGTTTG400.001730457137.46750359
TAAGGCG755.514994E-835.4928929
Produced by FastQC (version 0.11.5)