FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863025
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55730.6457518611859449No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20650.23927464441933893No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17970.20822108281915355No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14870.17230091828162566No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14330.16604385736218533No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13820.16013441093826944No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13710.15885982445467975No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13460.1559630369919759No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12500.14483937313519307No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12420.14391240114712783No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12370.14333304365458707No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11470.13290460878885316No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10730.12433011789924973No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10560.12236030242461111No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC10270.11900002896787464No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10210.11830479997682569No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10160.11772544248428493No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9150.10602242113496131No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8910.10324150517076562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG200.00954090548.45824419
GGTATCA24300.033.87921
GTATCAA54550.032.906321
TAGCGAG852.7110327E-630.25691851
TAACGGC1152.1918822E-929.29899436
CCGTATG450.00583217129.24208630
GTGGTAC450.00639303528.6976781
TATCAAC64550.027.602052
TAGCATC1104.6051355E-827.56769236
AACGCAG64450.027.5335396
CGCAGAG64050.027.5039948
ATCAACG65300.027.125733
TCAACGC65450.027.0651324
CAACGCA65550.027.023845
ATTAGGT857.275871E-526.93355657
GCTATAT607.9608714E-426.92124719
AGAGTAC65450.026.76778611
GCAGAGT66200.026.4645279
ACGCAGA67350.026.3479827
ATGGCAA5000.026.27457270
Produced by FastQC (version 0.11.5)