FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057337
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19200.18158827318064155No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13720.1297599535436668No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12440.11765406866495734No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11830.11188485790244737No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11330.1071559966217015No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11190.10583191546309266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45000.036.611531
GGTATCA21150.035.3495031
CGTTAAG400.002863590433.81714617
ATCAACG51550.031.6015723
TCAACGC51600.031.5084324
CAACGCA52350.031.1215885
AACGCAG52650.030.8800566
TATCAAC55450.029.6283342
ACGCAGA56350.028.8524447
CGCAGAG57500.028.2166068
TGGTATC9300.027.9885432
GCAGAGT58300.027.9453729
AGGCTCG2104.2220563E-827.8118370
AGAGTAC58450.027.75668311
ACGTAAT500.00831065827.19661130
CAGAGTA60550.027.01858910
GTGGTAT9800.026.9258821
GTAGTTT4100.026.9073870
ACGGGTA4500.026.29724516
CGCCGTA1307.650669E-926.061325
Produced by FastQC (version 0.11.5)