FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767884
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49400.6433263357486287No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45870.5973558506232712No Hit
GAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATATATGATGCCTC40500.5274234129113251No Hit
CTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA39620.5159633486307827No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34580.4503284350240401No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32430.42232941433862403No Hit
GATATATGATGCCTCCATTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA32410.4220689583322481No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32410.4220689583322481No Hit
GCCCAAAAGTGTACGAATAACTATGATATTGTTGGCAGTGATAAGTTGCA29830.3884701335097489No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27900.3633361288944684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27730.3611222528402728No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27480.3578665527605732No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACCTTGCAACTTA26380.34354147240989524No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26130.3402857723301957No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26040.3391137203015039No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAACGTCACCATCACTTGCCG25640.3339046001739846No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25320.3297373040719692No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24100.3138494876830355No Hit
TCATATATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC23960.31202629563840373No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23670.3082496835459522No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23530.3064264915013205No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23490.30590557948856856No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22340.2909293591219507No Hit
GTCTGCATCTGTAGGAGACAACGTCACCATCACTTGCCGGGCCAGTCAGA21800.2838970469497997No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21690.2824645389147319No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTG21680.28233431091154393No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACGTTGTCTCCTACAGA21480.2797297508477843No Hit
ATATCATAGTTATTCGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20890.2720462986596934No Hit
CTATGATATTGTTGGCAGTGATAAGTTGCAAGGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC20250.26371170645566255No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20010.260586234379151No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18420.23987998187226198No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18360.23909861385313408No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17780.23154538966823116No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC17760.23128493366185518No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT17750.23115470565866722No Hit
GTAGGAGACAACGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA17680.23024310963635133No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17640.22972219762359938No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17280.22503398950883208No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16670.21709008131436516No Hit
GTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT16350.21292278521234975No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16330.2126623292059738No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16260.21175073318365792No Hit
ACTTATCACTGCCAACAATATCATAGTTATTCGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAA16200.21096936516453005No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15890.20693229706570263No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC15650.20380682498919106No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15440.20107203692224346No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15370.2001604408999276No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15020.19560246078834825No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14740.19195607669908477No Hit
CACCATCAGCAGCCTGCAGCCTGATGACCTTGCAACTTATCACTGCCAAC14480.18857014861619725No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14390.18739809658750542No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14280.1859655885524376No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14240.1854446765396857No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14170.18453308051736983No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACCTTG14060.18310057248230202No Hit
GTGATAAGTTGCAAGGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13910.1811471524344823No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13900.1810169244312943No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13900.1810169244312943No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13850.18036578441535442No Hit
CCTTGCAACTTATCACTGCCAACAATATCATAGTTATTCGTACACTTTTG13830.18010532840897844No Hit
ATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA13810.17984487240260247No Hit
GCCTGATGACCTTGCAACTTATCACTGCCAACAATATCATAGTTATTCGTACACTTTTGGGCAGGGGACCAAG13690.1782821363643467No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13640.1776309963484068No Hit
ACCTTGATGGGACCCCACTTTCCAAAATGGAGGCATCATATATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTG13630.17750076834521883No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAACGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAA13580.1768496283292789No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13360.1739846122591433No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13340.17372415625276733No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13330.17359392824957937No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13220.17216142021451158No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13180.17164050820175963No Hit
GTTGCAAGGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGT13120.17085914018263174No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13080.1703382281698798No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12910.16812435211568413No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12890.16786389610930819No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAG12640.16460819602960863No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12630.16447796802642065No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12590.16395705601366875No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12580.16382682801048074No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATATATGATGCCTCCATTTTGGAAAGTGGGGTCCCAT12530.16317568799454085No Hit
CAATATCATAGTTATTCGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12380.16122226794672112No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12280.1599199879148413No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12180.1586177078829615No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11700.15236676372993838No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11320.14741809960879507No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11230.14624604758010326No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11200.1458553635705393No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11160.14533445155778738No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10830.14103692745258398No Hit
ATGATATTGTTGGCAGTGATAAGTTGCAAGGTCATCAGGCTGCAGGCTGC10790.14051601543983205No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10590.1379114553760724No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10580.13778122737288445No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10550.1373905433633205No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10530.13713008735694454No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10470.13634871933781667No Hit
GCTTGGTCCCCTGCCCAAAAGTGTACGAATAACTATGATATTGTTGGCAGTGATAAGTTGCAAGGTCATCAGG10400.13543712331550078No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10350.13478598329956087No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10160.13231165123898922No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC9910.1290559511592897No Hit
GTCAGAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATATATGATG9870.12853503914653777No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9820.12788389913059786No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9810.12775367112740987No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9710.12645139109553005No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9690.1261909350891541No Hit
GTTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACGTTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA9610.12514911106365023No Hit
GCCTGCAGCCTGATGACCTTGCAACTTATCACTGCCAACAATATCATAGTTATTCGTACACTTTTGGGCAGGGGA9550.12436774304452235No Hit
CTGTAGGAGACAACGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGT9400.12241432299670263No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCC9320.12137249897119877No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9200.11980976293294299No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9130.11889816691062713No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATATATGATGCCTCCATTTTGGAAAGTGGGGTCCCATCAAGGTT9110.11863771090425115No Hit
CCCCTAAGCCCCTGATATATGATGCCTCCATTTTGGAAAGTGGGGTCCCA9080.11824702689468723No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8940.11642383485005547No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTG8850.11525178282136364No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8780.11434018679904777No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACC8770.1142099587958598No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTGTACGA8710.11342859077673191No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG8620.11225653874804006No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC8560.11147517072891218No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8540.1112147147225362No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT8440.1099124346906564No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC8360.10887061066515255No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8330.10847992665558862No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTC8290.10795901464283669No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8230.1071776466237088No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAACGTCACCATCACTTGCCGG8160.10626605060139291No Hit
GTATCAACGCAGAGTACGGGAGTCAGACTCAGTCAGGACACAGCATGGAC8150.10613582259820493No Hit
TTCGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG8150.10613582259820493No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC8100.10548468258226502No Hit
GCTGATACCAGGCCAACCAGTTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACGTTGTCTCCTACAGATG8040.10470331456313715No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG8030.10457308655994915No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG8030.10457308655994915No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT8030.10457308655994915No Hit
GTGTACGAATAACTATGATATTGTTGGCAGTGATAAGTTGCAAGGTCATC7840.1020987544993775No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG7810.10170807048981356No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC7720.10053601846112173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)