FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767884
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49430.6437170197581926No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37920.4938245880888259No Hit
CTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA37480.4880945559485548No Hit
GAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATATATGATGCCTC37100.4831458918274114No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35220.4586630272280709No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32250.4199853102812404No Hit
GATATATGATGCCTCCATTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA30400.39589312969146384No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29350.3822191893567257No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28830.3754473331909507No Hit
GCCCAAAAGTGTACGAATAACTATGATATTGTTGGCAGTGATAAGTTGCA28460.3706288970729954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27070.352527204629866No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACCTTGCAACTTA25970.338202124279188No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25080.3266118319954576No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24060.31332857567028355No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23850.3105937876033359No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23630.30772877153320033No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23500.3060358074917566No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23430.3051242114694407No Hit
TCATATATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC22680.2953571112303421No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAACGTCACCATCACTTGCCG22550.29366414718889833No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21770.28350636294023573No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTG21510.2801204348573482No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21380.2784274708159045No Hit
CTATGATATTGTTGGCAGTGATAAGTTGCAAGGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC20780.2706137906246256No Hit
ATATCATAGTTATTCGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20740.27009287861187364No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20640.2687905985799939No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20430.2660558105130462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20340.2648837584843544No Hit
GTCTGCATCTGTAGGAGACAACGTCACCATCACTTGCCGGGCCAGTCAGA20040.26097691838871495No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA19800.25785144631220336No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACGTTGTCTCCTACAGA19590.2551166582452558No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18800.24482864599340526No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC18230.2374056498116903No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18130.2361033697798105No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17780.23154538966823116No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16490.21474597725698155No Hit
ACTTATCACTGCCAACAATATCATAGTTATTCGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAA16480.21461574925379354No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16350.21292278521234975No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16290.21214141719322188No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16120.2099275411390262No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT15790.20563001703382283No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15550.20250454495731127No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15200.19794656484573192No Hit
GTAGGAGACAACGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA15170.19755588083616799No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15170.19755588083616799No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15040.1958629167947242No Hit
GTGATAAGTTGCAAGGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14980.1950815487755963No Hit
GTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT14650.19078402467039293No Hit
CCTTGCAACTTATCACTGCCAACAATATCATAGTTATTCGTACACTTTTG14250.18557490454287365No Hit
GCCTGATGACCTTGCAACTTATCACTGCCAACAATATCATAGTTATTCGTACACTTTTGGGCAGGGGACCAAG14030.18270988847273806No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13930.18140760844085826No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAG13880.18075646842491835No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13590.17697985633246688No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC13380.17424506826551928No Hit
GTTGCAAGGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGT13370.1741148402623313No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13360.1739846122591433No Hit
ACCTTGATGGGACCCCACTTTCCAAAATGGAGGCATCATATATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTG13240.17242187622088753No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13210.17203119221132357No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13200.1719009642081356No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13140.1711195961890077No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13090.17046845617306783No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12920.16825458011887212No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12920.16825458011887212No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12880.1677336681061202No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12790.16656161607742836No Hit
ATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA12740.16591047606148845No Hit
CACCATCAGCAGCCTGCAGCCTGATGACCTTGCAACTTATCACTGCCAAC12650.16473842403279662No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12610.1642175120200447No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12600.1640872840168567No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATATATGATGCCTCCATTTTGGAAAGTGGGGTCCCAT12500.1627850039849769No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAACGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAA12450.16213386396903698No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12280.1599199879148413No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACCTTG12200.15887816388933745No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12110.15770611186064562No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12050.15692474384151775No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12000.1562736038255778No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11880.15471086778732204No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11760.15314813174906625No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11700.15236676372993838No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11630.1514551677076225No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11490.14963197566299077No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11290.14702741559923113No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11250.1465065035864792No Hit
CAATATCATAGTTATTCGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11250.1465065035864792No Hit
ATGATATTGTTGGCAGTGATAAGTTGCAAGGTCATCAGGCTGCAGGCTGC11070.14416239952909554No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10870.1415578394653359No Hit
GCTTGGTCCCCTGCCCAAAAGTGTACGAATAACTATGATATTGTTGGCAGTGATAAGTTGCAAGGTCATCAGG10860.14142761146214794No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10740.13986487542389214No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10700.13934396341114022No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC10650.1386928233952003No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10530.13713008735694454No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10390.1353068953123128No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT10320.13439529928999694No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAAT10170.1324418792421772No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10150.13218142323580123No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10150.13218142323580123No Hit
GCCTGCAGCCTGATGACCTTGCAACTTATCACTGCCAACAATATCATAGTTATTCGTACACTTTTGGGCAGGGGA9980.12996754718160555No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9960.12970709117522958No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9920.12918617916247765No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTG9810.12775367112740987No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9590.12488865505727428No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9590.12488865505727428No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9430.12280500700626658No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9310.1212422709680108No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9170.11941907892337904No Hit
GTTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACGTTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA9070.11811679889149923No Hit
GTCAGAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATATATGATG9060.11798657088831126No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG9010.11733543087237135No Hit
GTGTACGAATAACTATGATATTGTTGGCAGTGATAAGTTGCAAGGTCATC8990.11707497486599538No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8970.11681451885961942No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTGTACGAATAA8720.11355881877991988No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8630.11238676675122805No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCC8570.11160539873210015No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8560.11147517072891218No Hit
CCCCTAAGCCCCTGATATATGATGCCTCCATTTTGGAAAGTGGGGTCCCA8500.11069380270978428No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACC8460.11017289069703237No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACACTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG8430.10978220668746842No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC8410.10952175068109245No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT8380.1091310666715285No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC8230.1071776466237088No Hit
TTCGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG8230.1071776466237088No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC8160.10626605060139291No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8160.10626605060139291No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTGTACGA8120.10574513858864099No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTC8120.10574513858864099No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8050.10483354256632513No Hit
GTATCAACGCAGAGTACGGGAGTCAGACTCAGTCAGGACACAGCATGGAC8030.10457308655994915No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT7940.10340103453125732No Hit
CTGTAGGAGACAACGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGT7900.1028801225185054No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA7870.10248943850894145No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG7770.10118715847706164No Hit
GCTGATACCAGGCCAACCAGTTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACGTTGTCTCCTACAGATG7770.10118715847706164No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATATATGATGCCTCCATTTTGGAAAGTGGGGTCCCATCAAGGTT7740.10079647446749769No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7740.10079647446749769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG150.002519760767.834971
ATTGATT250.007253855551.9357369
ACCGATG200.007631920751.2739132
CGCCTCA306.709521E-445.43058429
ACGGGAT551.6732156E-743.18741616
GTATCAA17450.041.2063451
GTGGTAT1600.040.277011
GTAGTTT650.001231762740.15091370
GGAAACG1005.6347417E-639.14714470
CGATACT558.329313E-636.986434
ATCAACG20700.035.2168273
TATCAAC20500.035.068792
AACGCAG20750.034.798366
TCAACGC21000.034.711474
GCGCCTC400.002768132434.05064428
CAACGCA21350.033.983635
ACGGGTC701.1076772E-633.9329716
CTCGTCT400.002823627633.91306313
CCCGATA601.50994E-533.9108542
GATACTC601.5119624E-533.904235
Produced by FastQC (version 0.11.5)