FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591102
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC38310.6481114934478314No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36780.6222276358394998No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGAATCTCTGGCT32010.5415309032958779No Hit
GTCTTTATATATCACCAACACAGGAGCCTGACCTGACTTCTGCTGGTACC29410.4975452629157066No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAGTACCA29170.4934850499575369No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26400.4466234253986622No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAAATGCAATGCCAGAG26380.4462850743188147No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25580.4327510311249158No Hit
CTGCACAGACTCCGCGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT25260.42733741384735635No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25250.42716823830743256No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25090.42446142966865275No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAAATG23970.40551376919719434No Hit
CTCTCTGCACAGACTCCGCGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG22460.3799682626687103No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22120.37421629431130327No Hit
CATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT20640.3491783144025904No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGGTACTTATGTGGTGTTCGG20600.34850161224289544No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20370.3446105748246496No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCGCTGATGGTCAACGTGA20180.34139623956609855No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19480.32955395177143704No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18900.31974177045586044No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18160.307222780501504No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17870.3023166898437157No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGCAG17770.3006249344444783No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17620.2980873013456222No Hit
CCACATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCG17060.28861347110989305No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT16520.2794779919540113No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAAATGC16220.27440272575629926No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16210.2742335502163755No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16010.2708500394179008No Hit
CTATTACTGTCAATCAGCAGACAGCAGTGGTACTTATGTGGTGTTCGGCG15890.26881993293881595No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAGTACCAC15760.2666206509198074No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15570.26340631566125644No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15390.26036115594262915No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15080.2551167142049934No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14680.24834969260804396No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14650.24784216598827277No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14630.2475038149084253No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14540.24598123504911165No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14450.24445865518979804No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14400.24361277749017934No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13560.22940203213658558No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCGCTGATGGTCAACGT13440.22737192565750072No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGACTCCGCGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT13180.22297336161948364No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12950.2190823242012377No Hit
TCCTCACTCTCTGCACAGACTCCGCGGCCTCCTATGAGCTGACACAGCCA12170.20588663208718633No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11960.20233394574878785No Hit
GTACTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT11840.20030383926970302No Hit
GCAATATGCTTCTTGGTACCAGCAGAAGTCAGGTCAGGCTCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCC11410.1930292910529824No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11230.18998413133435516No Hit
CTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC11170.18896907809481275No Hit
GGATCACCTGCTCTGGAAATGCAATGCCAGAGCAATATGCTTCTTGGTACCAGCAGAAGTCAGGTCAGGCTC11050.1869389716157279No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10790.18254040757771078No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10300.17425080612144772No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10220.17289740180205787No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10060.1701905931632781No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10050.17002141762335435No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10020.16951389100358313No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9930.16799131114426952No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9780.16545367804541347No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG9780.16545367804541347No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9760.165115326965566No Hit
ATATGCTTCTTGGTACCAGCAGAAGTCAGGTCAGGCTCCTGTGTTGGTGA9750.16494615142564226No Hit
GCTTGGTCCCTCCGCCGAACACCACATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTT9740.16477697588571852No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAGTAC9740.16477697588571852No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9700.1641002737260236No Hit
CAGTCACGTTGACCATCAGCGGAGTCCAGGCAGAAGACGAGGCTGACTAT9670.1635927471062524No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGATTCGCTCAGGGATCCCTGAGGGCCTCTCA9610.16257769386670998No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9530.1612242895473201No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9350.15817912982869284No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9310.1575024276689979No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9280.1569949010492267No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC9070.15344221471082825No Hit
CTCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCT8920.15090458161197223No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8880.15022787945227728No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8650.14633684203403136No Hit
ACACAGGAGCCTGACCTGACTTCTGCTGGTACCAAGAAGCATATTGCTCT8540.14447591109487026No Hit
GGTCAGGCTCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG8530.14430673555494652No Hit
GTGAGAGGCCCTCAGGGATCCCTGAGCGAATCTCTGGCTCCAGCTCAGGG8490.14363003339525157No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8420.14244580461578543No Hit
GTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGC8160.1380472405777683No Hit
GGGATCCCTGAGGGCCTCTCA8140.13770888949792084No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGACTCCGCGGCCT8050.1361863096386072No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG8020.135678783018836No Hit
GCAGTGGTACTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA8020.135678783018836No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7950.13449455423936985No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAAATGCAATGCCAGAGCAATA7930.13415620315952237No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC7870.13314114991997997No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7850.13280279884013252No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7840.13263362330020878No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7820.1322952722203613No Hit
TATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGAATCTCTGGC7700.13026516574127645No Hit
GGGACAACAGTCACGTTGACCATCAGCGGAGTCCAGGCAGAAGACGAGGC7670.12975763912150526No Hit
GCCCTCAGGGATCCCTGAGCGAATCTCTGGCTCCAGCTCAGGGACAACAG7500.12688165494280174No Hit
CCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCGCTGATGGTCAACGTGA7490.12671247940287803No Hit
GCTCTGGAAATGCAATGCCAGAGCAATATGCTTCTTGGTACCAGCAGAAG7420.12552825062341186No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7350.12434402184394572No Hit
CTGTTGTCCCTGAGCTGGAGCCAGAGATTCGCTCAGGGATCCCTGAGGGCCTCTCA7340.12417484630402198No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT7200.12180638874508967No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC7180.1214680376652422No Hit
GCAGAAGTCAGGTCAGGCTCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGAAT7120.12045298442569981No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC7110.12028380888577607No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGACTCCGCGGCCTCC7100.12011463334585233No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT7100.12011463334585233No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT7010.1185920534865387No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7000.11842287794661496No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCC6950.11757700024699629No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6940.11740782470707255No Hit
GCTCAGGGACAACAGTCACGTTGACCATCAGCGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAG6930.11723864916714881No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT6840.1157160693078352No Hit
CAGGGATCCCTGAGCGAATCTCTGGCTCCAGCTCAGGGACAACAGTCACG6790.11487019160821652No Hit
GCATATTGCTCTGGCATTGCATTTCCAGAGCAGGTGATCCTGGCCGTCTG6720.11368596282875036No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG6620.111994207429513No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG6620.111994207429513No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG6610.11182503188958927No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC6610.11182503188958927No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6600.11165585634966554No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC6590.11148668080974182No Hit
AAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCT6580.11131750526981808No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6540.11064080311012313No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6400.10827234555119082No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6380.10793399447134334No Hit
GCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCT6320.10691894123180096No Hit
GACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCA6280.106242239072106No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC6190.10471965921279237No Hit
CCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT6190.10471965921279237No Hit
CAGCAGTGGTACTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC6130.10370460597324996No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6110.10336625489340248No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA5980.10116697287439393No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT5950.10065944625462273No Hit
CAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG5950.10065944625462273No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG5920.10015191963485151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1900.077.1607570
CCTCGTT401.0103596E-466.6388370
CCGTTCC354.2170723E-757.041699
GTCTTTA4250.053.279471
GCCCGTT253.068101E-453.238917
ACGTGTA200.00720774552.01917642
GTGGTAT655.456968E-1251.230261
ATTCTTA200.00828849550.21304728
ATATATC4750.047.6348157
TATATCA4800.047.138628
CTTTATA4900.046.859643
TATATAT4850.046.6565976
TTATATA4850.046.6565975
ATATCAC4800.046.44549
TCTTTAT4900.046.180512
TTTATAT5150.044.5848964
GGCCCGT307.5365015E-444.369516
TGGTATC752.5465852E-1144.369512
TATCACC5050.044.1498610
GGTATCA3200.043.705821
Produced by FastQC (version 0.11.5)