FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591102
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC35500.6005731667292616No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35010.5922835652729986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31190.5276585090221315No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGAATCTCTGGCT30370.5137861147483852No Hit
GTCTTTATATATCACCAACACAGGAGCCTGACCTGACTTCTGCTGGTACC28900.48891731037959607No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27760.4696312988282902No Hit
CTGCACAGACTCCGCGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT25990.43968722826178896No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAAATGCAATGCCAGAG25660.4341044354443057No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAGTACCA25530.4319051534252972No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24460.4138033706534574No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAAATG23370.39536323680177027No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22920.38775033750520216No Hit
CTCTCTGCACAGACTCCGCGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG22310.3774306295698543No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22160.3748929964709982No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19300.32650879205280986No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19190.32464786111364874No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19120.32346363233418257No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGGTACTTATGTGGTGTTCGG18290.3094220625205125No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18140.3068844294216565No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17930.30333174308325805No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17690.2992715301250884No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17650.29859482796539344No Hit
CATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT17430.29487296608707125No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAAATGC17200.29098192866882533No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGCAG17040.2882751200300456No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCGCTGATGGTCAACGTGA16310.2759253056156129No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16180.2737260235966043No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15920.26932745955858717No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15750.2664514753798837No Hit
CCACATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCG15130.25596259190461207No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15090.2552858897449171No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14890.2519023789464424No Hit
CTATTACTGTCAATCAGCAGACAGCAGTGGTACTTATGTGGTGTTCGGCG14650.24784216598827277No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14620.24733463936850156No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14250.2410751443913233No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13910.2353231760339163No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAGTACCAC13530.22889450551681437No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGACTCCGCGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT13430.22720275011757698No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13420.22703357457765327No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12980.2195898508210089No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12610.2133303558438307No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12140.2053791054674151No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11850.20047301480962676No Hit
GTACTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT11780.1992887860301606No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11710.19810455725069445No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG11480.19421351983244856No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCGCTGATGGTCAACGT11420.19319846659290613No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11360.19218341335336372No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11340.19184506227351625No Hit
TCCTCACTCTCTGCACAGACTCCGCGGCCTCCTATGAGCTGACACAGCCA11100.18778484931534659No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10850.18355546081725319No Hit
GGATCACCTGCTCTGGAAATGCAATGCCAGAGCAATATGCTTCTTGGTACCAGCAGAAGTCAGGTCAGGCTC10830.18321710973740574No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10580.17898772123931234No Hit
CTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10470.17712679030015124No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10440.17661926368038006No Hit
GCAATATGCTTCTTGGTACCAGCAGAAGTCAGGTCAGGCTCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCC10390.17577338598076137No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10340.17492750828114267No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10210.17272822626213413No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10120.1712056464028205No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9930.16799131114426952No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9910.16765296006442204No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9900.1674837845244983No Hit
CTCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCT9650.16325439602640493No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9650.16325439602640493No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAGTAC9620.16274686940663372No Hit
CAGTCACGTTGACCATCAGCGGAGTCCAGGCAGAAGACGAGGCTGACTAT9360.15834830536861658No Hit
GCTTGGTCCCTCCGCCGAACACCACATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTT9210.15581067226976053No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9040.15293468809105704No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8880.15022787945227728No Hit
ATATGCTTCTTGGTACCAGCAGAAGTCAGGTCAGGCTCCTGTGTTGGTGA8880.15022787945227728No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGATTCGCTCAGGGATCCCTGAGGGCCTCTCA8840.14955117729258233No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8710.14735189527357376No Hit
CTGCCTGGACTCCGCTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAG8660.1465060175739551No Hit
GGGATCCCTGAGGGCCTCTCA8400.14210745353593796No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8360.14143075137624303No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC8300.1404156981367006No Hit
ACACAGGAGCCTGACCTGACTTCTGCTGGTACCAAGAAGCATATTGCTCT8280.14007734705685312No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8140.13770888949792084No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC8140.13770888949792084No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG7970.13483290531921732No Hit
GGTCAGGCTCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG7860.13297197438005623No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7830.13246444776028504No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAAATGCAATGCCAGAGCAATA7740.1309418679009714No Hit
GCAGTGGTACTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA7700.13026516574127645No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC7700.13026516574127645No Hit
GCCCTCAGGGATCCCTGAGCGAATCTCTGGCTCCAGCTCAGGGACAACAG7610.12874258588196286No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC7590.12840423480211538No Hit
GTGAGAGGCCCTCAGGGATCCCTGAGCGAATCTCTGGCTCCAGCTCAGGG7440.12586660170325933No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7350.12434402184394572No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7320.12383649522417452No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7300.12349814414432704No Hit
GCTCTGGAAATGCAATGCCAGAGCAATATGCTTCTTGGTACCAGCAGAAG7300.12349814414432704No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7290.1233289686044033No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGACTCCGCGGCCT7260.1228214419846321No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC7250.12265226644470836No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCC7200.12180638874508967No Hit
CAGGGATCCCTGAGCGAATCTCTGGCTCCAGCTCAGGGACAACAGTCACG7110.12028380888577607No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT7070.11960710672608112No Hit
CTGTTGTCCCTGAGCTGGAGCCAGAGATTCGCTCAGGGATCCCTGAGGGCCTCTCA7040.11909958010630992No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGACTCCGCGGCCTCC6970.11791535132684376No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6910.11690029808730133No Hit
GCTCAGGGACAACAGTCACGTTGACCATCAGCGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAG6910.11690029808730133No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6890.11656194700745387No Hit
GGGACAACAGTCACGTTGACCATCAGCGGAGTCCAGGCAGAAGACGAGGC6870.1162235959276064No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6850.11588524484775894No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC6840.1157160693078352No Hit
TATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGAATCTCTGGC6820.11537771822798773No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT6770.11453184052836905No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6740.11402431390859784No Hit
GTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGC6610.11182503188958927No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG6560.1109791541899706No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT6510.11013327649035193No Hit
GCAGAAGTCAGGTCAGGCTCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGAAT6460.10928739879073324No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT6430.10877987217096204No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT6430.10877987217096204No Hit
CCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCGCTGATGGTCAACGTGA6350.10742646785157214No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6310.10674976569187722No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT6270.10607306353218227No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6260.10590388799225853No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC6140.1038737815131737No Hit
CGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAAAT6130.10370460597324996No Hit
GCATATTGCTCTGGCATTGCATTTCCAGAGCAGGTGATCCTGGCCGTCTG6110.10336625489340248No Hit
GAGCAATATGCTTCTTGGTACCAGCAGAAGTCAGGTCAGGCTCCTGTGTTGGTGATATATAAAGACAGTGAGAGG6060.10252037719378382No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT6050.10235120165386008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)