FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484847
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATGGTAGTGCACCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA54020.3638085270738332No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG53260.35869015460852194No Hit
GTATGGTAGTGCACCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT45950.30945949313296256No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43080.2901309023758003No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA41880.2820492616410984No Hit
GTGTTAGCACAGACTACGCAGATTCTTGGAAGGGCCGGTTCACCATCTCC34040.22924920884104558No Hit
GTGCTAACACCGCTTCCACTAATACCTGAGACCCACTCCAGCCCCTTCCC32320.21766552378797277No Hit
CTACCATACTGGTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC31990.21544307258592973No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT31650.21315327437776416No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31610.2128838863532741No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31590.2127491923410291No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC31130.20965123005939335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30680.20662061478388008No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT29370.19779815698183048No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG28750.19362264260223444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28640.19288182553488675No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT27610.18594508390426756No Hit
GTGTATTACTGTCACCAGTATGGTAGTGCACCTCCGCTCACTTTCGGCGG27200.1831838566532444No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24690.16627975811649282No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24100.16230628475526435No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC23490.15819811738179085No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC23130.15577362516138027No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23080.1554368901307677No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22630.15240627485525446No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22570.15200219281851934No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT22360.1505879056899465No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21720.14627769729810547No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTAGAGAAACCTACGTAGCCTGGTATC21150.14243891794912203No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC21010.1414960598634068No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC20840.14035116075932402No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC20000.13469401224503266No Hit
GTCCCAGACCCACTGCCAGTGAACCTGTCTGGGATGCCAGTGGCCCTTCT19600.132000132000132No Hit
GTGTTAGAGAAACCTACGTAGCCTGGTATCAGCAAAGACGTGGCCAGGCTCCCCGGCTCCTCATCTCAGGTGCAT19590.13193278499400948No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC19560.13173074397564194No Hit
GCCCTGGGATTTGCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA19430.13085523289604922No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19360.1303838038531916No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19330.13018176283482408No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT19330.13018176283482408No Hit
GAAATATATGGCCGTGTCCTCGGCTCTCAGGCGGTTCATTTGCAGATACA19230.12950829277359888No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18860.12701645354706578No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18580.12513073737563535No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT18260.12297563317971481No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA18090.12183073407563204No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17850.12021440592869163No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17680.11906950682460886No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTAGAGAAA17510.1179246077205261No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17400.11718379065317841No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC17250.11617358556134066No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA17120.11529807448174795No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17010.11455725741440029No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT16950.11415317537766517No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT16580.11166133615113207No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16310.10984296698582413No Hit
GTAGTGCACCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA16210.10916949692459897No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16200.10910214991847644No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16180.10896745590623141No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGA16130.10863072087561884No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16000.10775520979602612No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT15430.1039164304470427No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG15420.10384908344092017No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15380.1035796954164301No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15290.10297357236132747No Hit
TCTCAGGTGCATCCAGAAGGGCCACTGGCATCCCAGACAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACT15140.10196336726948972No Hit
AAGTAGTACCAGCCACTCTTCGAACTATCTTTCACACAGAAATATATGGC15130.10189602026336719No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA15070.1014919382266321No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15070.1014919382266321No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC15040.10128989720826456No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG14980.10088581517152945No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14910.10041438612867185No Hit
ACCATACTGGTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC14870.10014499810418179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24150.036.771171
CGCTGTA12600.036.3851470
GTGGTAT2850.035.8146321
AATGGTA5800.035.00501370
TATCAAC26550.032.9213072
ATCAACG27750.031.7331753
TCAACGC28300.031.1164514
CAACGCA28600.030.7910965
CGAAAAT450.00470903530.55142237
TTCATGG14050.030.2993770
AACGCAG29250.030.2230936
CGCAGAG30200.029.2713768
TCCGAGA12850.029.05148170
AGAGTAC30650.028.5059511
GCAGAGT31500.028.3852489
ACAGACG13450.028.24244170
AACGCAT500.00830280127.20261416
TAGGTGG15400.026.4942685
TCAGGTC14900.026.46699512
AAAACCG2007.907129E-726.197370
Produced by FastQC (version 0.11.5)