FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386224
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT17430.12573725458511756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2300.045.00095740
GGTATCA20050.044.7582631
TCTCGTA2300.043.34527638
CGTATGC2650.040.43936541
GTATCAA50100.039.2847981
GTGGTAT5600.037.63461
CTCGTAT2800.036.8988239
CGAGACA3550.033.49563623
ATCAACG59700.032.657013
TCAACGC60100.032.4396634
TATCAAC60600.032.285272
CAACGCA61250.031.9414965
AACGCAG62200.031.5082536
AATCTCG3300.031.17354436
ATCTCGT3550.029.98347737
GGCAATC3700.029.56761633
ACGCAGA67050.029.3272727
CGCAGAG67600.029.0886638
GCCGTCT3850.029.01078446
TGGTATC7150.028.978662
Produced by FastQC (version 0.11.5)