FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386224
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34130.246208404990824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.042.190491
GTGGTAT7250.038.3622551
GTATCAA50850.037.5530931
TGGTATC7800.036.075132
TTAACGG3350.032.71105635
ATCAACG60050.031.6086063
ACGTTAG800.00406469231.48214770
TCAACGC60700.031.3248394
CAACGCA61350.031.1034455
AACGCAG61550.031.0012596
TATCAAC61550.030.9551322
TAACGGC3500.030.3739436
ACGCAGA67000.028.4795157
CGCAGAG68800.027.7836728
AGAGTAC69050.027.43469211
CCGCTTA500.0080212727.39550835
CAGAGTA70500.026.96851510
GCAGAGT70850.026.8821569
GCGGTAC4500.025.52868744
CGGTACC4250.025.43932245
Produced by FastQC (version 0.11.5)