FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842705
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28220.33487400691819796No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26030.3088862650631004No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22540.2674720097780362No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19420.23044837754611636No Hit
GGGTTGGAGTGGATTGGCAGTATAAATTATGGTGGGACTACCTATTATAA19410.23032971205819355No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18470.21917515619344846No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18110.21490319862822693No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18110.21490319862822693No Hit
GACTTCACACTCACCATCACCAGTCTGCAGCCTGAAGATGTTGGAAGTTATTACTGTCATCAGAGTTACAGTTTT17990.2134792127731531No Hit
CAATAATAGATGGCCGTGTCTGCGGCGGTCACAGAGGAGAGGGTCAGTGA17770.2108685720388511No Hit
CTATTATTGTGCGAGACATGCTTCGTACCATTATGATAGTGGTCGGGGGTTCGACCCCTGGGGCCAGGGAACG17450.20707127642532086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17400.20647794898570673No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17290.20517262861855573No Hit
ACCTATTATAATCCGTCTCTCGAGAGTCGAGTCGTCATATCTGTCGACACTTCCAAGAACCACTTCTCACTGACC17100.20291798434802213No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16890.2004260091016429No Hit
CACCATCACCAGTCTGCAGCCTGAAGATGTTGGAAGTTATTACTGTCATC16780.1991206887344919No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC16750.19876469227072344No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16730.19852736129487783No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16500.19579805507265294No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16470.19544205860888447No Hit
GAGTTACAGTTTTCCGTACACTTTTGGCCAGGGGAGCAAGCTGGACATCAAGGGAACTGTGGCTGCACCAT16290.19330607982627374No Hit
GTGTGAAGTCTGTCCCAGATCCACTCCCACTGAACCTTGATGGGACCCCG15770.1871354744542871No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14700.17443826724654535No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14690.17431960175862252No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14510.17218362297601175No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACTTTCACTTGCCGGACAAGTCAAAGTGTGAACAC14450.17147163004847485No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14430.17123429907262921No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14160.16803033089871308No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14100.16731833797117615No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14050.16672501053156205No Hit
CTATCATAATGGTACGAAGCATGTCTCGCACAATAATAGATGGCCGTGTC14000.16613168309194795No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13530.16055440515957542No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13460.15972374674411569No Hit
CATCTATTCTGCCTCCAGTTTGCATACCGGGGTCCCATCAAGGTTCAGTG13420.1592490847924244No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13290.15770643344942772No Hit
CTTTTGGCCAGGGGAGCAAGCTGGACATCAAGGGAACTGTGGCTGCACCA13190.15651977857019952No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13090.15533312369097135No Hit
GAATAGATGAGGAGCTTGGGGGCTTTACCTGGTTTTTGCTGATACCAATT13080.15521445820304852No Hit
ATTATAATCCGTCTCTCGAGAGTCGAGTCGTCATATCTGTCGACACTTCCAAGAACCACTTCTCACTGACCCTCT12970.1539091378358975No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12940.15355314137212905No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGAAGCGACACCCAGATGACCCAGTC12810.15201049002913236No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12800.15189182454120956No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12490.14821319441560213No Hit
GTCTGCATCTGTGGGAGACAGAGTCACTTTCACTTGCCGGACAAGTCAAA12390.14702653953637396No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12160.14429723331414906No Hit
TTATGATAGTGGTCGGGGGTTCGACCCCTGGGGCCAGGGAACGCTGGTCACTGTCTCCTCAGCATCCCCGACCAG12160.14429723331414906No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12070.1432292439228437No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11960.14192392355569267No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11960.14192392355569267No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATGTCCAGCTTGCTCCCCTGGCCAAAAGTGTACGGAAAA11940.14168659257984703No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11820.1402626067247732No Hit
GTCTCTAGTGGCTCCATTCGGAGCAGTAGTTATTACTGGGGTTGGGTCCG11550.13705863855085706No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11500.13646531111124297No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11360.1348039942803235No Hit
CACCTGCAGTGTCTCTAGTGGCTCCATTCGGAGCAGTAGTTATTACTGGG11240.13338000842524964No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11130.13207468805809863No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGCGACAC11120.1319560225701758No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11110.13183735708225297No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11080.13148136061848453No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11070.1313626951305617No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10970.1301760402513335No Hit
GGCCAAAAGTGTACGGAAAACTGTAACTCTGATGACAGTAATAACTTCCA10950.12993870927548787No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10810.1282773924445684No Hit
ATAATAGATGGCCGTGTCTGCGGCGGTCACAGAGGAGAGGGTCAGTGAGA10800.12815872695664557No Hit
GTGCAGCCACAGTTCCCTTGATGTCCAGCTTGCTCCCCTGGCCAAAAGTG10720.127209403053263No Hit
GTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCATGGATCTCAGGTGCAAGAAAATGAAGTTCCTGTGGTTCCT10700.12697207207741737No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10480.12436143134311534No Hit
CTCCAACCCCGTCCCTGGGGGCTGGCGGACCCAACCCCAGTAATAACTAC10390.12329344195180995No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10330.12258144902427302No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGCGTTCCCTGGCCCCAGGGGTCGAACCCCCGACCA10310.12234411804842737No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10100.11985214280204816No Hit
GTCCCAGATCCACTCCCACTGAACCTTGATGGGACCCCGGTATGCAAACT10090.11973347731412534No Hit
ATAATAGGTAGTCCCACCATAATTTATACTGCCAATCCACTCCAACCCCGTCCCTGGGGGCTGGCGGACCCAACC9910.11759749853151459No Hit
GTGGGAGACAGAGTCACTTTCACTTGCCGGACAAGTCAAAGTGTGAACACCTATTTAAATTGGTATCAGCAAAAA9840.11676684011605486No Hit
GTCAAAGTGTGAACACCTATTTAAATTGGTATCAGCAAAAACCAGGTAAAGCCCCCAAGCTCCTCATCTATTCTG9800.11629217816436356No Hit
GTCCTGTCCCAACTGCAGCTGCAGGAGTCGGGTCCAGGACGGGTGCAGCC9750.11569885072474947No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9690.11498685779721256No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9630.11427486486967563No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9540.11320687547837024No Hit
GATGGGTCCTGTCCCAACTGCAGCTGCAGGAGTCGGGTCCAGGACGGGTGCAGCCTTCGGAGACCCTGTCACTCA9500.11273221352667898No Hit
GTATAAATTATGGTGGGACTACCTATTATAATCCGTCTCTCGAGAGTCGA9410.1116642241353736No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9410.1116642241353736No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGCGTTCCCTGG9340.11083356571991385No Hit
GTACGGGGCTTTCTGAGAGTCATGGATCTCAGGTGCAAGAAAATGAAGTT9230.10952824535276284No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGCGACACCCAGATGACCCAGTCTCCATCCTCC9200.10917224888899438No Hit
GTCCAGGACGGGTGCAGCCTTCGGAGACCCTGTCACTCACCTGCAGTGTC9150.10857892144938026No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8990.10668027364261516No Hit
CTGTAACTCTGATGACAGTAATAACTTCCAACATCTTCAGGCTGCAGACTGGTGATGGTGAGTGTGAAGTCTGTC8890.10549361876338693No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8840.10490029132377285No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8750.10383230193246748No Hit
GATCTGGGACAGACTTCACACTCACCATCACCAGTCTGCAGCCTGAAGAT8690.10312030900493055No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG8690.10312030900493055No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8660.1027643125411621No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8620.10228965058947082No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8490.10074699924647415No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8440.10015367180686006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGG150.002409984668.6019235
GCGAATA200.007641162751.25868229
CTACGCT1100.046.57664528
ACTACGC1150.044.53304327
CGAGACT1150.044.49074623
ACGAGAC1150.044.4881122
TCGCTAT405.342618E-542.6445624
GTTGTGA359.646701E-442.20126367
CACGAGA1250.040.92663221
TACGCTG1350.037.96939529
CTTATAC1350.037.9040261
CTCGCTA451.0707374E-437.8995323
GTATCAA14200.037.476941
TGCATCG655.725524E-736.91966234
GAGACTA1400.036.55248324
TTATACA1400.036.535132
GTGGTAT1500.036.3878631
CCGTCTT709.035539E-734.8290247
CGTATGC501.8307517E-434.59626841
GCTTGTA400.002743326434.1136251
Produced by FastQC (version 0.11.5)