FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842705
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28270.33546733435781206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22100.26225072830943214No Hit
GGGTTGGAGTGGATTGGCAGTATAAATTATGGTGGGACTACCTATTATAA22040.2615387353818952No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20260.24041627853163325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19720.23400834218380095No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19230.22819373327558282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18860.22380311022243846No Hit
GACTTCACACTCACCATCACCAGTCTGCAGCCTGAAGATGTTGGAAGTTATTACTGTCATCAGAGTTACAGTTTT17810.21134323399054233No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17460.20718994191324366No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17240.20457930117894163No Hit
ACCTATTATAATCCGTCTCTCGAGAGTCGAGTCGTCATATCTGTCGACACTTCCAAGAACCACTTCTCACTGACC17240.20457930117894163No Hit
GTGTGAAGTCTGTCCCAGATCCACTCCCACTGAACCTTGATGGGACCCCG17050.20232465690840803No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17020.2019686604446396No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16910.20066334007748857No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16570.19662871348811267No Hit
CTATTATTGTGCGAGACATGCTTCGTACCATTATGATAGTGGTCGGGGGTTCGACCCCTGGGGCCAGGGAACG16490.19567938958473013No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16110.19117010104366297No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16040.19033944262820324No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15820.18772880189390118No Hit
CAATAATAGATGGCCGTGTCTGCGGCGGTCACAGAGGAGAGGGTCAGTGA15600.18511816115959914No Hit
CACCATCACCAGTCTGCAGCCTGAAGATGTTGGAAGTTATTACTGTCATC15380.1825075204252971No Hit
GAGTTACAGTTTTCCGTACACTTTTGGCCAGGGGAGCAAGCTGGACATCAAGGGAACTGTGGCTGCACCAT14570.17289561590354868No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACTTTCACTTGCCGGACAAGTCAAAGTGTGAACAC14470.17170896102432048No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14420.17111563358470638No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14380.17064097163301511No Hit
ATTATAATCCGTCTCTCGAGAGTCGAGTCGTCATATCTGTCGACACTTCCAAGAACCACTTCTCACTGACCCTCT14040.16660634504363925No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13520.1604357396716526No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13500.16019840869580695No Hit
GTGGGAGACAGAGTCACTTTCACTTGCCGGACAAGTCAAAGTGTGAACACCTATTTAAATTGGTATCAGCAAAAA13500.16019840869580695No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13430.15936775028034722No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGAAGCGACACCCAGATGACCCAGTC13210.1567571095460452No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13160.1561637821064311No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12980.15402780332382032No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12940.15355314137212905No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12890.15295981393251493No Hit
CTTTTGGCCAGGGGAGCAAGCTGGACATCAAGGGAACTGTGGCTGCACCA12610.149637180270676No Hit
CTATCATAATGGTACGAAGCATGTCTCGCACAATAATAGATGGCCGTGTC12560.14904385283106186No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12550.14892518734313906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12250.14536522270545446No Hit
GTCTGCATCTGTGGGAGACAGAGTCACTTTCACTTGCCGGACAAGTCAAA12250.14536522270545446No Hit
CATCTATTCTGCCTCCAGTTTGCATACCGGGGTCCCATCAAGGTTCAGTG12200.14477189526584036No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12170.1444158988020719No Hit
GTCTCTAGTGGCTCCATTCGGAGCAGTAGTTATTACTGGGGTTGGGTCCG12090.14346657489868936No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11940.14168659257984703No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11940.14168659257984703No Hit
GAATAGATGAGGAGCTTGGGGGCTTTACCTGGTTTTTGCTGATACCAATT11780.13978794477308193No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11470.1361093146474745No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11440.13575331818370603No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11400.13527865623201477No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11360.1348039942803235No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11090.13160002610640734No Hit
TTATGATAGTGGTCGGGGGTTCGACCCCTGGGGCCAGGGAACGCTGGTCACTGTCTCCTCAGCATCCCCGACCAG11040.13100669866679324No Hit
GTGCAGCCACAGTTCCCTTGATGTCCAGCTTGCTCCCCTGGCCAAAAGTG10870.1289893853721053No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10840.12863338890833687No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10810.1282773924445684No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10800.12815872695664557No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATGTCCAGCTTGCTCCCCTGGCCAAAAGTGTACGGAAAA10750.12756539951703147No Hit
CACCTGCAGTGTCTCTAGTGGCTCCATTCGGAGCAGTAGTTATTACTGGG10710.12709073756534017No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10710.12709073756534017No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10590.12566675171026634No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10550.12519208975857507No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10530.12495475878272942No Hit
GTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCATGGATCTCAGGTGCAAGAAAATGAAGTTCCTGTGGTTCCT10240.12151345963296765No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10120.12008947377789381No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10100.11985214280204816No Hit
GTCCTGTCCCAACTGCAGCTGCAGGAGTCGGGTCCAGGACGGGTGCAGCC9970.11830949145905151No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGCGACAC9850.11688550560397766No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9810.11641084365228639No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGCGTTCCCTGGCCCCAGGGGTCGAACCCCCGACCA9800.11629217816436356No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9740.11558018523682664No Hit
ATAATAGATGGCCGTGTCTGCGGCGGTCACAGAGGAGAGGGTCAGTGAGA9670.11474952682136691No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9640.11439353035759844No Hit
GGCCAAAAGTGTACGGAAAACTGTAACTCTGATGACAGTAATAACTTCCA9610.11403753389382998No Hit
ATAATAGGTAGTCCCACCATAATTTATACTGCCAATCCACTCCAACCCCGTCCCTGGGGGCTGGCGGACCCAACC9590.11380020291798434No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9560.11344420645421589No Hit
CTCCAACCCCGTCCCTGGGGGCTGGCGGACCCAACCCCAGTAATAACTAC9550.11332554096629306No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9550.11332554096629306No Hit
GTCCCAGATCCACTCCCACTGAACCTTGATGGGACCCCGGTATGCAAACT9500.11273221352667898No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9450.11213888608706488No Hit
GTATAAATTATGGTGGGACTACCTATTATAATCCGTCTCTCGAGAGTCGA9170.10881625242522591No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9110.108104259497689No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC9030.10715493559430643No Hit
GATGGGTCCTGTCCCAACTGCAGCTGCAGGAGTCGGGTCCAGGACGGGTGCAGCCTTCGGAGACCCTGTCACTCA9020.10703627010638361No Hit
GTACGGGGCTTTCTGAGAGTCATGGATCTCAGGTGCAAGAAAATGAAGTT8770.10406963290831311No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGCGTTCCCTGG8750.10383230193246748No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGCGACACCCAGATGACCCAGTCTCCATCCTCC8730.10359497095662183No Hit
GAGCAGTAGTTATTACTGGGGTTGGGTCCGCCAGCCCCCAGGGACGGGGT8710.1033576399807762No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8540.10134032668608825No Hit
GTCCAGGACGGGTGCAGCCTTCGGAGACCCTGTCACTCACCTGCAGTGTC8500.10086566473439698No Hit
GTCAAAGTGTGAACACCTATTTAAATTGGTATCAGCAAAAACCAGGTAAAGCCCCCAAGCTCCTCATCTATTCTG8440.10015367180686006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2000.047.648671
GTATCAA14350.037.4738161
ATGGCAA1359.459163E-734.61900770
ATATTAG400.002774628334.0347671
TACATCG400.002780287334.0206265
GAGTTTA3450.033.5415081
TATCAAC16300.032.561552
AGTTTAT3700.031.2640532
ATCAACG16950.030.907763
TCAACGC16900.030.7979134
CAGGTAT450.004939331730.2531241
AATTCCC450.004949380630.2405555
AACGCAG17350.030.195196
CAACGCA17300.030.0876055
GGGTCAT6150.029.31156370
ACGCAGA18300.028.8135767
CGCAGAG18300.028.6276828
AGAGTAC18400.028.47209511
GCAGAGT18950.027.6457259
GACAGGT1005.646725E-727.2278121
Produced by FastQC (version 0.11.5)