FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910826
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21220.23297534325985425No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20100.2206788124186178No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17500.192133294394319No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16510.18126403945429753No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16260.17851927810580726No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15570.17094373678397412No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14680.16117238638334874No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14550.1597451104821338No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14220.15612202550212664No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13480.14799753191059545No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13330.1463506751015013No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13000.1427275901214941No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12730.13976324786512462No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10530.11560934799841023No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9800.10759464486081863No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9740.10693590213718097No Hit
CTATAGTAATTACCCCTGGACGTTCGGCCAGGGGACCACGGTGGATGTCAGACGAACTGTGGCTGCACCAT9360.10276386488747577No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9180.10078763671656278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG200.00810513950.5002865
GTGGTAT3600.048.6646581
GGTATCA14750.043.8552971
TGGTATC4200.040.0928042
TATCGAT350.001310621339.6526642
GTATCAA33550.037.1554071
ATCAACG40050.031.0206093
AACGCAG40600.030.7645456
TCAACGC40600.030.5970154
CAACGCA40900.030.5388875
TATCAAC42550.029.443482
ACGCAGA43400.028.6245867
CGCAGAG43800.028.3631768
GTCGACC500.007452583427.81039443
AGAGTAC44650.027.66938411
TTAGTCG550.00769056327.63156167
GGGATAT950.00809744127.33882370
CAGAGTA45450.027.18384710
TGTCGTA500.00845978527.09722729
GCCTAAT1457.393108E-526.86746470
Produced by FastQC (version 0.11.5)