FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638832
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11960.18721667042352294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11360.17782452976682445No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9790.15324842838179678No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9370.14667392992210784No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8460.1324291832594485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7590.1188105793072357No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7580.11865404362962408No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7350.11505372304455631No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT7330.11474065168933303No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC7320.11458411601172139No Hit
CTTTTGGCCAGGGGACTAAATTGGAGATCAAAGGAACTGTGGCTGCACCA7130.1116099381371002No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6880.10769654619680918No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6670.1044092969669647No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6640.10393968993412978No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG6590.10315701154607157No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6500.10174819044756682No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6440.10080897638189697No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6430.10065244070428532No Hit
CTAGAAACCCCGTACACTTTTGGCCAGGGGACTAAATTGGAGATCAAAGG6400.1001828336714504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGTA200.00594071154.62511450
CTACGCT2950.045.5569928
CGAGACT3000.045.48024723
TATACAC3400.043.8930053
CCCACGA3250.041.92589619
TCGTATG1400.041.90767340
ACTACGC3250.041.21481327
CGTGTTG350.001109416741.0172148
CGTATGC1650.039.991141
GAGACTA3450.039.57285724
ACGAGAC3450.039.5418422
ATACACA3800.039.272694
CACGAGA3500.038.95253421
TACGCTG3600.037.40538829
ATCTCCG3750.037.19810
TACACAT4150.036.7422875
TTATACA3800.036.7114262
ACACATC4050.036.0473986
GCCGTCT1500.035.5300546
GAATTGA800.00228657435.39652670
Produced by FastQC (version 0.11.5)