FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638832
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33230.5201680567034839No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12420.19441731159365844No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9930.15543992786835975No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9760.15277882134896184No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9550.1494915721191174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8370.1310203621609437No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7870.12319357828036166No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7240.11333183059082826No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7120.11145340245948858No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6910.1081661532296441No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6790.1062877250983044No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6660.10425276128935307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATC451.4109959E-436.1616361
ACGGGTA1153.1795935E-928.29375516
GTATCAA34250.028.2219091
TACGTAT1256.122613E-428.15053470
TAGTCAC854.901418E-628.03971538
GGTATCA17050.027.1053181
GGGAGTA600.00941404926.50767764
ATTCACT1605.456968E-1226.4349042
CGGGTAC759.164701E-526.03433417
TATCAAC36800.025.7276152
CAGAGTA36100.025.57957510
AGAGTAC36350.025.491111
CGCAGAG36600.025.4078048
TATGGTT650.001217673625.0290916
CCTTATA650.001218230225.0271282
AACGCAG38150.024.8016516
ATCAACG38450.024.610073
TCAACGC38550.024.5443084
CAACGCA38750.024.4176275
ACTCCCT951.66267E-523.95645512
Produced by FastQC (version 0.11.5)