FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095370
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64660.5903028200516721No Hit
GTAATAATCAGCCTCATCGTCAGACCGGAGGCCACTGATGGCCAGGGAGG61540.5618192939372084No Hit
GATTATTACTGTGCAGCTTGGGATGACACCCTGAATAGTTGGATGTTCGG58800.5368049152341219No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47690.4353780001278107No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46170.4215014104823027No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44050.4021472196609365No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42500.38799674995663563No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCC39250.3583264102540694No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGGTGTCATCCCAAG37340.34088937984425355No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAACTATTCAGG35730.32619115002236687No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34510.3150533609647882No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCC34140.31167550690634216No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCAC33800.30857153290668904No Hit
GTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGT33440.305284972201174No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32240.29432976984945725No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31310.28583948802687675No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31120.2841049143211883No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28830.2631987364999954No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC28130.25680820179482733No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26320.24028410491432117No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25420.23206770315053363No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25410.23197640979760265No Hit
GCCCCCACACTCCTCATCTATAGGACTACTGAACGGACCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGT23920.21837370021088762No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22960.20960953832951423No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22870.20878789815313545No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22110.20184960333038152No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22010.20093666980107178No Hit
CCTATGAACGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21920.20011502962469305No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21590.1971023489779709No Hit
CTATAGGACTACTGAACGGACCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC20670.1887033605083214No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20660.18861206715539042No Hit
GTGTGGGGGCCGTCCCTGGCAACTGACGATACCAGGAGACATAATTATAT20600.18806430703780458No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19710.17993919862694796No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19550.1784785049800524No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19250.17573970439212322No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19180.1751006509216064No Hit
CTCCTCATCTATAGGACTACTGAACGGACCTCAGGGGTCCCTGGCCGATT18980.17327478386298692No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18590.16971434309867897No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18380.16779718268712857No Hit
GACCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAG18050.16478450204040643No Hit
CCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT17920.1635976884523038No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17190.15693327368834276No Hit
AGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGG17160.15665939362954984No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16690.1523686060417941No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGGTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG16480.15045144563024365No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTCCTCACTCACTGTGCAGGGTCCTGGGC16040.14643453810128085No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGG15700.14333056410162776No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15660.14296539068990385No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15570.14214375051352512No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15390.14050047016076758No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15230.13903977651387203No Hit
CCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGTCATCTCTTGTTCTGG15100.13785296292576937No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14970.13666614933766674No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14750.13465769557318533No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTC14550.13283182851456585No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14160.1292713877502579No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14070.12844974757387914No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14000.12781069410336235No Hit
CTACTGAACGGACCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCT13930.12717164063284553No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13850.12644129380939775No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAA13780.12580224033888093No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT13740.12543706692715703No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13630.12443284004491632No Hit
GTCCTATAGATGAGGAGTGTGGGGGCCGTCCCTGGCAACTGACGATACCA13630.12443284004491632No Hit
GATGAGGAGTGTGGGGGCCGTCCCTGGCAACTGACGATACCAGGAGACAT13510.12333731980974465No Hit
GGCCATCAGTGGCCTCCGGTCTGACGATGAGGCTGATTATTACTGTGCAG13360.12196791951578005No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTCTGTAGGGGC13300.12142015939819423No Hit
GAATAGTTGGATGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13290.12132886604526323No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG13210.12059851922181546No Hit
CCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGGTGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATCGT13200.12050722586888449No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12820.1170380784575075No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12630.11530350475181902No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12470.11384281110492345No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12470.11384281110492345No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12450.11366022439906151No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12240.11174306398751108No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12170.11110401051699426No Hit
GCTTGGGATGACACCCTGAATAGTTGGATGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG12060.11009978363475355No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC12020.10973461022302966No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCTGACGATG11750.10726968969389339No Hit
CTTCAGGTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTC11720.10699580963510047No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG11700.10681322292923853No Hit
ATCCCAAGCTGCACAGTAATAATCAGCCTCATCGTCAGACCGGAGGCCAC11660.10644804951751463No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11600.1059002893999288No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC11550.10544382263527392No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11420.10425700904717128No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC11370.10380054228251641No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11280.10297890210613765No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG11230.1025224353414828No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC11120.10151820845924209No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA11110.10142691510631112No Hit
ATTCAGGGTGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATCGTCAG11060.10097044834165624No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA11030.10069656828286333No Hit
CCCCTGAGGTCCGTTCAGTAGTCCTATAGATGAGGAGTGTGGGGGCCGTCCCTGGCAACTGACGATACCAGGAGA11030.10069656828286333No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGT11010.10051398157700138No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA10990.10033139487113944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)