FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095370
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC59980.5475775308799766No Hit
GTAATAATCAGCCTCATCGTCAGACCGGAGGCCACTGATGGCCAGGGAGG59200.5404566493513607No Hit
GATTATTACTGTGCAGCTTGGGATGACACCCTGAATAGTTGGATGTTCGG55170.5036654281201786No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49870.45527995106676283No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44850.40945068789541433No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40910.373481106840611No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCC40270.36763833225302867No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40170.3667253987237189No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39700.3624346111359632No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGGTGTCATCCCAAG39230.35814382354820745No Hit
GTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGT34420.3142317207884094No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAACTATTCAGG32330.29515141002583606No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32260.2945123565553192No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCC32140.29341683632014753No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCAC31960.29177355596739No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28880.2636552032646503No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28500.2601860558532733No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27360.24977861361914236No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27160.24795274656052294No Hit
GTGTGGGGGCCGTCCCTGGCAACTGACGATACCAGGAGACATAATTATAT26110.23836694450277077No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC25290.23088088956243094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25090.2290550225038115No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24310.2219341409751956No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23500.21453937938778678No Hit
GCCCCCACACTCCTCATCTATAGGACTACTGAACGGACCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGT23320.21289609903502926No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23170.21152669874106464No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22240.20303641691848417No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21750.1985630426248665No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21620.19737622903676383No Hit
CTCCTCATCTATAGGACTACTGAACGGACCTCAGGGGTCCCTGGCCGATT21260.1940896683312488No Hit
CCTATGAACGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21080.19244638797849128No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21000.1917160411550435No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19870.18139989227384354No Hit
CTATAGGACTACTGAACGGACCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC19360.1767439312743639No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19090.17427901074522764No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18500.16889270292230024No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17920.1635976884523038No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17560.16031112774678877No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17490.15967207427627195No Hit
GACCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAG17330.15821138062937637No Hit
AGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGG16880.15410317974748258No Hit
CCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT16780.15319024621817284No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16680.1522773126888631No Hit
GTCCTATAGATGAGGAGTGTGGGGGCCGTCCCTGGCAACTGACGATACCA16440.15008627221851978No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16230.14816911180696934No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGGTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG15400.14059176351369856No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15210.1388571898080101No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15010.13703132274939062No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14910.13611838922008088No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14780.13493157563197825No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGG14710.13429252216146143No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14550.13283182851456585No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTC14480.13219277504404905No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13900.1268977605740526No Hit
GATGAGGAGTGTGGGGGCCGTCCCTGGCAACTGACGATACCAGGAGACAT13830.1262587071035358No Hit
CCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGTCATCTCTTGTTCTGG13640.12452413339784729No Hit
CCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGGTGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATCGT13580.12397637328026147No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13530.12351990651560661No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13180.12032463916302255No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13070.11932041228078184No Hit
GAATAGTTGGATGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13020.11886394551612697No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTCTGTAGGGGC13000.11868135881026502No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12970.1184074787514721No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12900.11776842528095528No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAA12850.11731195851630043No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12800.11685549175164556No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12730.11621643828112875No Hit
GGCCATCAGTGGCCTCCGGTCTGACGATGAGGCTGATTATTACTGTGCAG12570.11475574463423319No Hit
CTACTGAACGGACCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCT12550.11457315792837125No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC12430.11347763769319955No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA12020.10973461022302966No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11890.10854779663492702No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11860.1082739165761341No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11600.1059002893999288No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG11590.10580899604699782No Hit
GCTTGGGATGACACCCTGAATAGTTGGATGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG11500.10498735587061907No Hit
CCCCTGAGGTCCGTTCAGTAGTCCTATAGATGAGGAGTGTGGGGGCCGTCCCTGGCAACTGACGATACCAGGAGA11440.10443959575303322No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11360.10370924892958544No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCTGACGATG11300.1031614888119996No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC11200.10224855528268986No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11200.10224855528268986No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11160.10188338187096597No Hit
ATCCCAAGCTGCACAGTAATAATCAGCCTCATCGTCAGACCGGAGGCCAC11100.10133562175338012No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTCCTCACTCACTGTGCAGGGTCCTGGGC11010.10051398157700138No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10990.10033139487113944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG200.007532233451.4462134
GTATCAA32400.037.2453581
GGTATCA13550.037.233711
GCCAGAG25950.034.34033270
GTGGTAT5250.032.4656871
TATCAAC36750.032.1697652
TCAACGC38700.030.4579874
ATCAACG38850.030.3403913
CAACGCA39250.029.8589615
AACGCAG39600.029.5937046
ACCCGAG900.00594906229.12194870
CGCAGAG41450.028.438558
AGAGTAC42050.028.19223211
ACGCAGA41700.028.1046567
TACCGCC500.00761265127.6901141
CAGAGTA43200.027.44174610
GCAGAGT43600.027.1887459
AGTGATA6000.026.20975170
AGCCAGA25900.025.59216169
ACGGGGT2800.025.56205416
Produced by FastQC (version 0.11.5)