FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128665
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC64640.5727120093207462No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTACTACTATCTGCATATGAGCAG59650.528500485086363No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTACTACTATCTGCATATGAGCAGCAGTAATAAT50450.4469882560369995No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG50140.4442416483190318No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48250.4274962012643256No Hit
GATTATTACTGCTGCTCATATGCAGATAGTAGTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT46560.41252275918895337No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT43590.38620848524584356No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAAAGATTGTCAGGGAGG42040.37247544665600507No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41340.36627342922833617No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39520.3501481839163968No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC39290.3481103781901627No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC38350.33978195478729295No Hit
CCCAAGTACTACTATCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCC37790.33482034084515777No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCAGCCAGGCAAAGCCCCCCAA37500.3322509336251235No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37400.3313649311354565No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36740.3255173147036543No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCAGCCAGGCAAAGC36570.3240111104712204No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35960.3186064952842517No Hit
TAGTAGTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA35630.3156826870683507No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC34620.306734061922714No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATA34020.30141804698471203No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33020.2925580220880421No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32000.2835207966934387No Hit
GTACTACTATCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGG31590.27988818648580405No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30780.2727115663195014No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30270.2681929536221997No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT30090.26659814914079905No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG29070.2575609237461957No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC28350.25118170582059335No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27880.24701749411915846No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27380.2425874816708235No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27110.2401952749487226No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27060.23975227370388907No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26580.23549946175348752No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26480.23461345926382052No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC25670.22743683909751786No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC25210.22336122764504968No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24820.2199058179353484No Hit
GTAATAGACAGCCGTGTCCTCGGCTCCCAGACTGTTCAAGTCCAGATAAA24800.21972861743741498No Hit
GTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC24310.21538720523804675No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24280.21512140449114664No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTACTACTAT22370.198198756938507No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC21290.18862993005010342No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21180.18765532731146975No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20970.18579472208316905No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCG20950.18561752158523565No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20810.18437711809970184No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20620.18269371336933457No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC20610.18260511312036787No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT20370.18047870714516706No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19990.1771118976844325No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19250.1705554792608967No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC19130.16949227627329633No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18940.16780887154292903No Hit
GTAGTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT18840.16692286905326204No Hit
CCCCTGAGGGCCACTTATTGACCTCAAAAATCATGAGTTGGGGGGCTTTGCCTGGCTGCTGTTGGTACCAGGAGA18830.16683426880429533No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18760.16621406706152844No Hit
GTCAATAAGTGGCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTCCCTGACAAT18620.16497366357599463No Hit
CTTATTGACCTCAAAAATCATGAGTTGGGGGGCTTTGCCTGGCTGCTGTTGGTACCAGGAGACAAGGTTATAACT18030.15974624888695937No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC17880.1584172451524589No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17590.1558478379324246No Hit
GTACCAACAGCAGCCAGGCAAAGCCCCCCAACTCATGATTTTTGAGGTCA17200.1523924282227233No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTACTA17150.15194942697788982No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16910.14982302100268902No Hit
GCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTGCA16800.14884841826405532No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT16440.14565880930125413No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16430.14557020905228746No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16170.14326660257915325No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16070.14238060008948625No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGTACTACTATCTGCATATGAGCAG16010.14184899859568606No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15960.14140599735085255No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15900.14087439585705236No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG15740.13945679187358517No Hit
CCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTCCCTGACAATCTTTGGGCTCCAGC15650.13865938963288485No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15380.136267182910784No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC15230.1349381791762835No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC15230.1349381791762835No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15040.13325477444591619No Hit
GTCTATTACTGTGCAAGAGCATACGGGACTAGCATGTTAGATAGCCGGCA14750.13068536722588192No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC14390.12749575826308074No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14370.1273185577651473No Hit
GCCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGATAGTAGTA14270.12643255527548034No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14150.12536935228787993No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13830.12253414432094555No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13690.12129374083541175No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13590.12040773834574474No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13560.12014193759884466No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC13550.12005333734987796No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13490.11952173585607775No Hit
TCCTCAGGCTGGAGCCCAAAGATTGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAGCGATGAGAAACC13470.11934453535814436No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13450.11916733486021094No Hit
GGCTGGAGCCCAAAGATTGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCC13430.11899013436227755No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG13300.11783833112571046No Hit
GAGTTATAACCTTGTCTCCTGGTACCAACAGCAGCCAGGCAAAGCCCCCC13170.11668652788914335No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGAC13090.11597772589740976No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13030.11544612440360956No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGATCCAGACACGGAGGCAGG12960.11482592266084267No Hit
GTCTGGCAGCACGGCCTCCCTGACAATCTTTGGGCTCCAGCCTGAGGACG12940.11464872216290928No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12940.11464872216290928No Hit
GGCAAAGCCCCCCAACTCATGATTTTTGAGGTCAATAAGTGGCCCTCAGG12900.1142943211670425No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC12830.11367411942427559No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12770.11314251793047539No Hit
GTCCAGGAGATCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC12700.1125223161877085No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC12670.11225651544080838No Hit
GCAGTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAAAGATTGTCAGGG12560.1112819127021747No Hit
GTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG12560.1112819127021747No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCT12550.111193312453208No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC12440.1102187097145743No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12410.1099529089676742No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12400.10986430871870749No Hit
GGTACCAACAGCAGCCAGGCAAAGCCCCCCAACTCATGATTTTTGAGGTC12240.1084467047352403No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12180.1079151032414401No Hit
CCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAG12130.10747210199660662No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12090.1071177010007398No Hit
TCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGAT12080.10702910075177312No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12040.10667469975590631No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11870.10516849552347242No Hit
CATATGCAGATAGTAGTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC11790.10445969353173883No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG11710.10375089154000523No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11640.10313068979723834No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT11570.10251048805447144No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11410.10109288407100422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)