FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128665
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC61280.5429423256679351No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTACTACTATCTGCATATGAGCAG54540.48322575786437955No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG49910.44220384259279766No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48270.427673401762259No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTACTACTATCTGCATATGAGCAGCAGTAATAAT46900.4155351676538211No Hit
GATTATTACTGCTGCTCATATGCAGATAGTAGTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT45310.40144772806811585No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT41780.3701718401828709No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC39000.3455409709701284No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAAAGATTGTCAGGGAGG38900.3446549684804614No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCAGCCAGGCAAAGCCCCCCAA37350.330921929890623No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37110.3287955239154222No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37010.3279095214257552No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCAGCCAGGCAAAGC36910.3270235189360882No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36670.3248971129608874No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36610.32436551146708725No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35290.31267027860348284No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34440.3051392574413134No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33760.2991144405115778No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33720.29876003951571106No Hit
TAGTAGTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA33480.29663363354051026No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33220.29433002706737604No Hit
CCCAAGTACTACTATCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCC32900.29149481910044167No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATA32490.28786220889280695No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC30720.27217996482570117No Hit
GTACTACTATCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGG30570.27085096109120066No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29100.25782672449309585No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28450.2520677083102604No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT27750.2458656908825914No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC27740.24577709063362466No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27500.2436506846584239No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27430.24303048291565701No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC27100.2401066746997559No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26710.2366512649900546No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC25960.23000624631755218No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25770.22832284158718485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25630.22708243810165107No Hit
GTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC24350.21574160623391353No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24010.21272919776904572No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC23820.21104579303867846No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23480.20803338457381065No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22460.1989961591792073No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCG22380.19828735718747367No Hit
GTAATAGACAGCCGTGTCCTCGGCTCCCAGACTGTTCAAGTCCAGATAAA22160.19633815171020633No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22100.19580655021640608No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC20690.18331391511210146No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20330.18012430614930028No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20280.1796813049044668No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20060.1777320994271994No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19960.1768460969375324No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19790.1753398927050985No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT19250.1705554792608967No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTACTACTAT19210.17020107826502992No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC18830.16683426880429533No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18830.16683426880429533No Hit
CCCCTGAGGGCCACTTATTGACCTCAAAAATCATGAGTTGGGGGGCTTTGCCTGGCTGCTGTTGGTACCAGGAGA18330.16240425635596034No Hit
GTCAATAAGTGGCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTCCCTGACAAT18320.16231565610699367No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18300.16213845560906026No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT18300.16213845560906026No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18040.15983484913592608No Hit
GTAGTACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT18010.15956904838902597No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18010.15956904838902597No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTACTA17740.15717684166692508No Hit
CTTATTGACCTCAAAAATCATGAGTTGGGGGGCTTTGCCTGGCTGCTGTTGGTACCAGGAGACAAGGTTATAACT17590.1558478379324246No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16800.14884841826405532No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG16780.1486712177661219No Hit
GCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTGCA16490.14610181054608762No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16480.14601321029712094No Hit
GTACCAACAGCAGCCAGGCAAAGCCCCCCAACTCATGATTTTTGAGGTCA16160.14317800233018654No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC15630.13848218913495147No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15500.13733038589838437No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15500.13733038589838437No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC15320.13573558141698377No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15310.1356469811680171No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC15090.1336977756907497No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGTACTACTATCTGCATATGAGCAG15010.13298897369901608No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC14820.1313055689686488No Hit
CCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTCTGGCAGCACGGCCTCCCTGACAATCTTTGGGCTCCAGC14620.12953356398931481No Hit
GGCTGGAGCCCAAAGATTGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCC14430.12785015925894752No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14080.12474915054511303No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13940.12350874705957923No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13820.12244554407197886No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13820.12244554407197886No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13650.12093933983954494No Hit
GAGTTATAACCTTGTCTCCTGGTACCAACAGCAGCCAGGCAAAGCCCCCC13500.11961033610504446No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC13470.11934453535814436No Hit
GCCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGATAGTAGTA13370.11845853286847735No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13290.11774973087674376No Hit
GTCTATTACTGTGCAAGAGCATACGGGACTAGCATGTTAGATAGCCGGCA13280.11766113062777707No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13270.11757253037881037No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13210.11704092888501016No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC13190.11686372838707677No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13160.11659792764017667No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13130.11633212689327657No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGATCCAGACACGGAGGCAGG13110.11615492639534318No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13020.11535752415464287No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13020.11535752415464287No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGAC12820.11358551917530887No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12710.11261091643667519No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12690.1124337159387418No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12580.1114591132001081No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12510.11083891145734119No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC12470.11048451046147438No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG12470.11048451046147438No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12440.1102187097145743No Hit
GGTACCAACAGCAGCCAGGCAAAGCCCCCCAACTCATGATTTTTGAGGTC12240.1084467047352403No Hit
TCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGAT12200.10809230373937352No Hit
TCCTCAGGCTGGAGCCCAAAGATTGTCAGGGAGGCCGTGCTGCCAGACTTGGAGCCAGAGAAGCGATGAGAAACC12160.10773790274350671No Hit
GTCTGGCAGCACGGCCTCCCTGACAATCTTTGGGCTCCAGCCTGAGGACG12160.10773790274350671No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTC12120.10738350174763991No Hit
GTCCAGGAGATCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC11980.10614309826210613No Hit
CCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAG11860.10507989527450573No Hit
GTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG11820.10472549427863892No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC11740.10401669228690533No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11470.10162448556480443No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT11400.10100428382203754No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG11380.10082708332410414No Hit
TCTTTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT11300.10011828133237055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)