FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428385
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21440.15009958799623352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.041.4431951
GTGGTAT7900.038.9924051
TGGTATC7800.037.7457922
GTATCAA49550.036.3440781
TCAACGC59500.029.9609074
ATCAACG59750.029.7799783
CAACGCA61850.028.8772285
AACGCAG62150.028.6289836
TATCAAC62300.028.626382
ACGCAGA65400.027.2062857
CGCAGAG66350.026.7657648
AGAGTAC69850.025.46856911
GCAGAGT70650.025.1819429
CAGAGTA71750.024.74787110
GTCGATC800.002339943822.37396657
TAACGGC3550.022.18961336
TTAACGG3550.022.1487635
AGTACGG58050.022.08659213
GAGTACG59700.021.473912
TATATCG800.003250877621.1417685
Produced by FastQC (version 0.11.5)