FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669265
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT119390.7152249642806865No Hit
CTCCTAAGCTGCTCATTTACTGGGCGTCTGCCCGGGAATCCGGGGTCCCT102410.6135035479687168No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC86450.5178926054281375No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTGCCCGGGAA84440.5058513777021623No Hit
CTTTTATACACCTCCAACAATAAGAACTACTTAGGTTGGTACCAGCACAA80300.48105004298298953No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC79460.47601788811243273No Hit
GCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAACCTAAGTAGTTCTTATTGTTGGAGGTGTATAAAAGACT76570.4587048790934933No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC76230.4566680545030298No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG73390.4396545785120996No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT70080.4198254920578817No Hit
GTTTATTACTGTCACCAACATTATGAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA67600.4049686538686188No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCA63620.3811258248390759No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC62750.37591395015171347No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAACATTATGAAACT59080.3539282258958284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC58340.34949513708129026No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA57200.34266578404267745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC57160.34242615762027No Hit
GTCCTGGTTTGTGCTGGTACCAACCTAAGTAGTTCTTATTGTTGGAGGTG55230.3308641827391097No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG54370.3257122146573492No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT54170.3245140825453119No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT53830.3224772579548484No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT52720.3158276247330412No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT50480.30240854507822307No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC49880.29881414874211104No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG48950.29324283442113747No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC48630.2913258230418777No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCAC47820.2864733879881265No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG47500.2845563766088668No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44660.2675429006179367No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA43710.2618517730857593No Hit
GAGTGAATTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG43580.26107298721293504No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT42400.2540040077519148No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA41630.249391199120571No Hit
GCTCATTTACTGGGCGTCTGCCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATT38840.23267725615765023No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38820.23255744294644648No Hit
TTATGAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT38060.22800454092070463No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCACCATCAGCAGCC37250.22315210586695342No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA37030.22183416054371236No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG37020.22177425393811048No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGC36520.21877892365801715No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACACCTCCAA36250.21716144530676676No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG36110.21632275282834063No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA36060.21602321980033126No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG35680.21374676878746032No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA33800.2024843269343094No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAACATTATGAAACTCCGT33370.19990834289342913No Hit
GAGTTTCATAATGTTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAATT33350.19978852968222544No Hit
GTCAGGGACCCCGGATTCCCGGGCAGACGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTGTGCTGGTA33350.19978852968222544No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC33270.19930927683741048No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCA33210.19894983720379925No Hit
ATGAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA33150.19859039757018807No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC32680.19577478710690036No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT32500.19469646820606676No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTGCCCGGGAATCCGGGGTCCCTGA32490.19463656160046489No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAACATTATGAAACTCCGTGGACGTT32270.1933186162772238No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT31790.19044309920833422No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA31610.18936478030750062No Hit
ACTTAGGTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGCTGCTCATT31130.186489263238611No Hit
GTTTCATAATGTTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAATT30900.18511141130976808No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAACCTA30840.18475197167615687No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30410.1821759876352766No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA29860.17888112432717393No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG29460.17648486010309927No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA28970.17354943642860782No Hit
CTACTTAGGTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTGCCCGGGAATC28290.16947578724768086No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT28110.16839746834684727No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC28060.16809793531883793No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACACCTCCAACAATAAGAACTA27920.1672592428404118No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27850.16683989660119874No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT27740.1661809239395782No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT27450.16444363237712406No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC27440.1643837257715222No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC27440.1643837257715222No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC27120.16246671439226246No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC27090.16228699457545687No Hit
GGTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT26940.16138839549142886No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG26910.16120867567462327No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC26780.16042988980179898No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC26520.15887231805615046No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA26310.15761427933851127No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGTTTCATAATGTTGGTGACAGTAATAAACTGCCACATCTTCAGC26280.15743455952170565No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG26210.1570152132824926No Hit
GCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTGCCCGGGAATCCGGGGTCCCTGACCGATT25990.15569726795925154No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25770.15437932263601045No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG25590.15330100373517686No Hit
TTATTACTGTCACCAACATTATGAAACTCCGTGGACGTTCGGCCAAGGGA25290.15150380556712084No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA25060.15012595363827794No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24990.14970660739906486No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT24650.1476697828086014No Hit
CATTTACTGGGCGTCTGCCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCA24520.14689099693577712No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC24140.14461454592290618No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA24080.144255106289295No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC23850.14287725436045207No Hit
GGTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTGCCCGGGAATCCGGGGTC23530.14096024298119234No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23310.13964229765795125No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23300.13958239105234937No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23080.13826444572910832No Hit
GTGCTGGTACCAACCTAAGTAGTTCTTATTGTTGGAGGTGTATAAAAGAC23010.13784509948989526No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT22910.1372460334338766No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT22870.13700640701146913No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22780.1364672475610523No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG22720.13610780792744112No Hit
CCTCCAACAATAAGAACTACTTAGGTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG22300.13359173049216272No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT22220.1331124776473478No Hit
ACATTATGAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT22190.1329327578305422No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGCAGA22010.1318544389297086No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC21530.128978921860819No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21260.12736144350956857No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGC21240.12724163029836486No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA21210.12706191048155926No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC21180.12688219066475365No Hit
GGTTTGTGCTGGTACCAACCTAAGTAGTTCTTATTGTTGGAGGTGTATAA21150.12670247084794806No Hit
CCTAAGTAGTTCTTATTGTTGGAGGTGTATAAAAGACTCTGGCTGGACTT20820.12472555286308644No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC20310.12167031597739124No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC20270.12143068955498378No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA20130.12059199707655764No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCACCATCAG20080.12029246404854832No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19720.11813582624688113No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG19590.11735704037405684No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT19450.1165183478956307No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19370.11603909505081578No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19110.11448152330516724No Hit
CAATAAGAACTACTTAGGTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTGC18980.11370273743234298No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG18760.1123847921091019No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG18140.10867058256178617No Hit
GAACTACTTAGGTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGCTGC17960.10759226366095258No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT17580.10531581264808165No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC17420.10435730695845177No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC17390.10417758714164618No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGCAGACGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTGTG17200.1030393616352107No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16960.10160160310076591No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG16940.10148178988956216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACCA200.006183363554.07956363
TAGACTG200.007863685550.8900224
GGTATCA13750.045.696111
GTATCAA38750.041.4571651
GTGGTAT3450.040.362211
GACAGGT1350.040.2528271
TATCAAC42350.037.509572
ATCAACG44850.035.4038773
TATTTGA400.002321266635.29762654
TCAACGC45350.035.0114364
AACGCAG45850.034.926666
CAACGCA45950.034.6291435
AGTAAGA800.00292410333.67238270
CGCAGAG48300.033.082798
ACGCAGA48450.033.051387
AGAGTAC49050.032.3684711
TGGTATC4200.032.3266262
GCAGAGT49250.032.3068779
CAGAGTA49500.032.14274610
GAGTTTA2950.031.0850771
Produced by FastQC (version 0.11.5)