FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164666
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT89720.7703496109614258No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67250.5774187621172078No Hit
CTCCTAAACTACTCATTTACTGGGCATCTTTCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGT67100.5761308392277271No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC63010.541013475107885No Hit
ATATTATGCTCTTCCTCCATACACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCAT60040.515512601896166No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58500.5022899268974969No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53530.4596167484927009No Hit
GTTTTATATCACTCCAACAATAAGAACTATTTAGCTTGGTACCAGCAGAA53280.4574702103435663No Hit
GTTTATTACTGTCAGCAATATTATGCTCTTCCTCCATACACTTTTGGCCA49610.42595903031427035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48350.415140478042632No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46570.39985712642079363No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGCTCTTCCTCCATACA45430.3900689124607398No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45050.3868061744740552No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT44450.3816544829161322No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44220.37967966781892837No Hit
GTTTAGGAGGCTGTCCGGGTTTCTGCTGGTACCAAGCTAAATAGTTCTTATTGTTGGAGTGATATAAAACACT42900.36834594639149765No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41120.3530625947696593No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40400.3468805649001516No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGA40170.3449057498029478No Hit
CTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCA40170.3449057498029478No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39940.342930934705744No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39480.3389813045113363No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38690.3321982439600709No Hit
GCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT37710.32378381441546333No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37660.32335450678563643No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGTGACAGATTTCACTCTCAC37360.32077866100667485No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36070.30970252415714034No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35160.30188912529429035No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35030.3007729254567404No Hit
GTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGCCAAAAGTGTATGGAGGAAGAGCATAATATTG32960.28299958958190585No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28630.24582154883889457No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28520.24487707205327536No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28220.24230122627431383No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27660.2374929808202523No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCC27550.2365485040346331No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27170.2332857660479485No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26990.23174025858057162No Hit
GGCCAAAAGTGTATGGAGGAAGAGCATAATATTGCTGACAGTAATAAACT26930.23122508942477932No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25360.21774482984821397No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25280.21705793764049092No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT25020.21482553796539094No Hit
ACTCATTTACTGGGCATCTTTCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGTGACAGATTT24770.21267899981625635No Hit
GTGTATGGAGGAAGAGCATAATATTGCTGACAGTAATAAACTGCCACATC24450.20993143098536401No Hit
CTATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTTTCCGGGAATC23940.20555249316112945No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23830.20460801637551024No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT23790.2042645702716487No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23380.20074424770706792No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23260.19971390939548334No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23050.19791081735021027No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22970.1972239251424872No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22860.19627944835686797No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22780.19559255614914492No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCACAGACCCGCTGCCAATGAATCGGTCAGGGACC22530.1934460180000103No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22440.19267326426632184No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22250.19104189527297954No Hit
GTCAGGGACCCCGGATTCCCGGAAAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCGGGTTTCTGCTGGTA22080.189582249331568No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATATCACTCCAA22060.18941052627963725No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGCTCTTCCTC22000.18889535712384495No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21960.18855191101998342No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21680.18614778829295267No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC21350.183314357936095No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGT21140.18151126589082192No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20920.17962231231958345No Hit
CAGCAATATTATGCTCTTCCTCCATACACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCA20800.17859197400799887No Hit
ATTATGCTCTTCCTCCATACACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCAT20760.1782485279041373No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20610.17696060501465657No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20540.1763595743328989No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT20500.17601612822903734No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20480.1758444051771066No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20310.17438475923569505No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19650.1687178985219797No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19540.16777342173636048No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19500.16742997563249895No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19370.16631377579494894No Hit
GTGATGGTGAGAGTGAAATCTGTCACAGACCCGCTGCCAATGAATCGGTC19250.16528343748336433No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18990.16305103780826435No Hit
TTATTACTGTCAGCAATATTATGCTCTTCCTCCATACACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAA18980.16296517628229895No Hit
GCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGCCAAAAGTGTATGG18920.16245000712650667No Hit
ATTCATTGGCAGCGGGTCTGTGACAGATTTCACTCTCACCATCACCAGCC18730.16081863813316435No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18660.16021760745140667No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18590.15961657676964897No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18250.1566972848868259No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18220.15643970030892976No Hit
GAACTATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCTCCTAAACTAC18200.156267977256999No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18190.1561821157310336No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18070.155151777419449No Hit
GTAAATGAGTAGTTTAGGAGGCTGTCCGGGTTTCTGCTGGTACCAAGCTA17950.1541214391078644No Hit
GAGTGAAATCTGTCACAGACCCGCTGCCAATGAATCGGTCAGGGACCCCG17870.15343454690014133No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17510.1503435319653875No Hit
CTCCAACAATAAGAACTATTTAGCTTGGTACCAGCAGAAACCCGGACAGC17170.14742424008256444No Hit
ATATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16860.14476253277763754No Hit
ATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCACAGA16730.14364633294008755No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16670.14313116378429525No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16650.1429594407323645No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGCTCTT16570.14227254852464138No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT16470.14141393326498755No Hit
GTCTGTGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG16470.14141393326498755No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16310.1400401488495414No Hit
GAGGAAGAGCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCC16300.13995428732357604No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16280.13978256427164526No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16030.13763602612251066No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16030.13763602612251066No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG15990.1372925800186491No Hit
GAGCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT15900.13651982628496068No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15760.1353177649214453No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT15360.13188330388282993No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG15330.13162571930493377No Hit
GACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT15330.13162571930493377No Hit
CTCTTCCTCCATACACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGA15300.13136813472703762No Hit
GGATCTCTGGTGCCTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG15000.1287922889480761No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT14800.12707505842876843No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG14670.12595885859121844No Hit
GTGTTTTATATCACTCCAACAATAAGAACTATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCTCCTAAACTA14610.12544368943542614No Hit
AACCCGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTTTCCGGGAA14550.12492852027963383No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14550.12492852027963383No Hit
GTACCAGCAGAAACCCGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTTTCCGGGAATCCGGGGTCCCTGA14530.12475679722770305No Hit
ATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCTCCTAAACTACTCATT14520.12467093570173768No Hit
GCAGAAACCCGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTTTCCGGGAATCCGGGGTCCCTGACCGATT14390.12355473586418766No Hit
CTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14310.1228678436564646No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC14200.12192336687084537No Hit
GTCACAGACCCGCTGCCAATGAATCGGTCAGGGACCCCGGATTCCCGGAA14050.12063544398136462No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG13860.1190040749880223No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA13840.11883235193609154No Hit
GGTGATGGTGAGAGTGAAATCTGTCACAGACCCGCTGCCAATGAATCGGT13540.11625650615713003No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCGAGCTTGGTCCCCTGGC13390.11496858326764928No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13300.1141958295339608No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA13270.11393824495606465No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13250.11376652190413389No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT13180.1131654912223762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13030.11187756833289544No Hit
CAATAAGAACTATTTAGCTTGGTACCAGCAGAAACCCGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTTT12650.10861483034621085No Hit
GGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACA12550.107756215086557No Hit
GGCTGGTGATGGTGAGAGTGAAATCTGTCACAGACCCGCTGCCAATGAAT12450.10689759982690317No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG12450.10689759982690317No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACATCGTGATGA12260.10526623083356086No Hit
GAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG12120.10406416947004549No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12120.10406416947004549No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC12090.10380658489214933No Hit
CATTTACTGGGCATCTTTCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGTGACAGATTTCA12010.10311969268442626No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC11990.1029479696324955No Hit
CCTCCATACACTTTTGGCCAGGGGACCAAGCTCGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTC11950.10260452352863396No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG11660.10011453927563782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)