FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911845
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGTTACCCTTACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT89850.985364837225625No Hit
CTCTTATACTGTTGGCAGAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTC72090.7905948927723462No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43290.474751739604867No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41750.45786290433132826No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29470.3231908931890837No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28050.3076180710537427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26530.2909485713032368No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25840.28338149575859933No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24980.2739500682681815No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23350.2560742231409944No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22900.2511391738727525No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTGACAA22850.25059083506517005No Hit
CATCTATGATGCATCGAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA22410.2457654535584447No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22370.24532678251237872No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC22170.24313342728204904No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20560.2254769176778948No Hit
CAGTATAAGAGTTACCCTTACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT19880.21801950989477378No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19280.21143944420378463No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19220.21078143763468574No Hit
GTGTAAGGGTAACTCTTATACTGTTGGCAGAAGTAAGTTGCAAAATCTTC18430.20211768447488335No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18400.20178868119033389No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18400.20178868119033389No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17160.18818987876228965No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT17010.18654486233954237No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16840.1846805103937621No Hit
CCCTTACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGG16400.17985512888703672No Hit
AGTCAGGGCATTGACAAATATTTAGGCTGGTTTCAGCAGAAAACAGGGAG16270.1784294479873224No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG16120.17678443156457513No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15750.17272672438846515No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15670.17184938229633326No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTACTTCTGCCAACAGTATAAGAGTTAC15300.16779167512022328No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14810.16241795480591548No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT14810.16241795480591548No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT14790.1621986192828825No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14370.1575925732991901No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC14350.15737323777615714No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14300.15682489896857468No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14150.1551798825458274No Hit
GAGTTACCCTTACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCAT14140.15507021478431093No Hit
TCATAGATGAGGGACTTTGGGGCTCTCCCTGTTTTCTGCTGAAACCAGCC13730.15057383656213502No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC13590.1490384879009042No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13320.1460774583399591No Hit
GCCCCAAAGTCCCTCATCTATGATGCATCGAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT13280.14563878729389315No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13190.14465177744024477No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13140.14410343863266237No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12890.1413617445947502No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTACTTCTGCCAACAGTATAAGAGTTACCCTTACACTTT12770.14004573145655236No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG12680.139058721602904No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12600.1381813795107721No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12580.13796204398773915No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12560.13774270846470618No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCGTCA12530.13741370518015672No Hit
GGGTAACTCTTATACTGTTGGCAGAAGTAAGTTGCAAAATCTTCAGGCTG12280.13467201114224459No Hit
CTTATACTGTTGGCAGAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCCGC12230.13412367233466213No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12060.13225932038888188No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11900.1305046362046181No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC11570.12688560007457408No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11410.1251309158903103No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11330.1242535737981784No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11260.12348589946756303No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCGTCA11210.1229375606599806No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11190.1227182251369476No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11170.12249888961391464No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGTAAGGGTAACT11170.12249888961391464No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11100.12173121528329925No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10830.11877018572235412No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10740.11778317586870575No Hit
CTACATGGACTGGGTCCGTCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTGGCCGTGTCAGACCTAAGACTAAGA10710.11745417258415629No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10710.11745417258415629No Hit
CTACTACATGGACTGGGTCCGTCAGGCTCCAGGGAAGGGGCTGGAGTGGG10660.11690583377657388No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10560.11580915616140901No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10540.11558982063837604No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10520.11537048511534306No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10370.11372546869259578No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10360.1136158009310793No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10360.1136158009310793No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10320.11317712988501336No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10320.11317712988501336No Hit
GTCCCTCATCTATGATGCATCGAATTTGCAAAGTGGGGTCCCATCAAGGT10290.11284812660046388No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10180.11164178122378256No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT10150.11131277793923308No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10090.11065477137013417No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10080.11054510360861769No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9980.10944842599345282No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9970.10933875823193634No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9860.10813241285525499No Hit
ACCCAGTCTCCCTCGTCACTGTCTGCATCTGTAGGAGACAGAGTCACCAT9650.1058293898634088No Hit
AAACAGGGAGAGCCCCAAAGTCCCTCATCTATGATGCATCGAATTTGCAA9650.1058293898634088No Hit
CAATAATAGACGGCCGTGTCCTCGATTTTAAGGCCGTTCATTTGCAGAAA9610.10539071881734287No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9510.104294041202178No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT9490.10407470567914504No Hit
TATTTAGGCTGGTTTCAGCAGAAAACAGGGAGAGCCCCAAAGTCCCTCAT9460.10374570239459557No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA9250.10144267940274937No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTG9140.10023633402606803No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9130.10012666626455155No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9130.10012666626455155No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9120.10001699850303507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG408.7552326E-751.31826423
CGTATGC451.818893E-646.15295841
TCGTATG404.9363593E-543.2204240
CGCTCAT504.0344694E-641.10647228
GGTATCA7750.041.0636251
GTATCAA20800.038.003591
CACGAGA557.832989E-637.3203321
AGGCCGC15100.036.3890246
CGCTGAT15250.035.94334450
AATCTGT16550.035.82629468
GGCCGCT15300.035.7159247
ATGCCGT601.2892808E-534.70331644
TATCAAC22500.034.6607362
TTCAGGC15750.034.3276536
ATCTCCG601.4247029E-534.2009210
GCTGATG16200.033.86588751
GCAGGCC16500.033.44137644
ATCAACG23550.033.2605973
TGCAGGC16400.033.19252843
AGAGTGA16800.033.09586761
Produced by FastQC (version 0.11.5)