FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911845
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGTTACCCTTACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT82560.9054170390801068No Hit
CTCTTATACTGTTGGCAGAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTC76250.8362166815632044No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44220.4849508414259003No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34780.38142447455433764No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30400.33338999501011685No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29950.328454945741875No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25030.27449840707576395No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24130.2646283085392802No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21730.23830804577532363No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC20290.2225158881169497No Hit
CATCTATGATGCATCGAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA20220.2217482137863343No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20200.2215288782633013No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTGACAA20170.22119987497875188No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19920.2184581809408397No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19560.21451014152624623No Hit
GTGTAAGGGTAACTCTTATACTGTTGGCAGAAGTAAGTTGCAAAATCTTC19460.2134134639110814No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19230.2108911053962022No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19150.21001376330407034No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18770.20584638836644384No Hit
CAGTATAAGAGTTACCCTTACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT18700.20507871403582845No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18660.20464004298976252No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18120.1987179838678723No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18110.1986083161063558No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17830.1955376187838942No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17210.18873821756987208No Hit
CCCTTACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGG16110.17667476380305863No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15760.17283639214998162No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15170.16636599422050896No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTACTTCTGCCAACAGTATAAGAGTTAC14630.16044393509861873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14630.16044393509861873No Hit
AGTCAGGGCATTGACAAATATTTAGGCTGGTTTCAGCAGAAAACAGGGAG14340.15726357001464064No Hit
GAGTTACCCTTACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCAT13990.15342519836156363No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT13860.1519995174618493No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG13690.15013516551606906No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13680.15002549775455257No Hit
GGGTAACTCTTATACTGTTGGCAGAAGTAAGTTGCAAAATCTTCAGGCTG13630.14947715894697017No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13280.14563878729389315No Hit
TCATAGATGAGGGACTTTGGGGCTCTCCCTGTTTTCTGCTGAAACCAGCC13130.14399377087114587No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT13090.1435550998250799No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13090.1435550998250799No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13050.14311642877901398No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13030.14289709325598102No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT13020.14278742549446452No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12980.1423487544483986No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12830.1407037380256513No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12770.14004573145655236No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12480.1368653663725743No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC12110.1328076591964643No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT11960.13116264277371703No Hit
CTTATACTGTTGGCAGAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCCGC11960.13116264277371703No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC11820.12962729411248622No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11640.12765327440518948No Hit
GCCCCAAAGTCCCTCATCTATGATGCATCGAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT11490.12600825798244217No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11210.1229375606599806No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG11190.1227182251369476No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTACTTCTGCCAACAGTATAAGAGTTACCCTTACACTTT11170.12249888961391464No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCGTCA11000.12063453766813438No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10980.12041520214510142No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10930.11986686333751899No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10890.11942819229145303No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10850.1189895212453871No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10850.1189895212453871No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC10820.11866051796083764No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10800.11844118243780467No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCGTCA10760.11800251139173874No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGTAAGGGTAACT10720.11756384034567277No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10510.11526081735382658No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10400.11405447197714523No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10330.11328679764652985No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10150.11131277793923308No Hit
CTACATGGACTGGGTCCGTCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTGGCCGTGTCAGACCTAAGACTAAGA10050.11021610032406824No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9990.10955809375496932No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9950.10911942270890337No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9930.10890008718587041No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9930.10890008718587041No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9880.10835174837828798No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9850.1080227450937385No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9750.10692606747857367No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9720.10659706419402422No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9700.10637772867099123No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9700.10637772867099123No Hit
CTACTACATGGACTGGGTCCGTCAGGCTCCAGGGAAGGGGCTGGAGTGGG9430.10341669911004613No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9340.10242968925639774No Hit
GTCCCTCATCTATGATGCATCGAATTTGCAAAGTGGGGTCCCATCAAGGT9330.10232002149488126No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9290.10188135044881533No Hit
CAATAATAGACGGCCGTGTCCTCGATTTTAAGGCCGTTCATTTGCAGAAA9290.10188135044881533No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9190.10078467283365046No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9180.10067500507213398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG100.009499274131.4800470
TCTGCGC200.00766249451.22289311
CTACGTA200.007665818651.2172749
AATGGTA3000.048.20934770
GGTATCA9650.043.2173731
GTATCAA23050.036.1863441
AATCTGT20200.035.04096268
TACCCTT20500.034.81491511
ACCCTTA20650.034.72548312
TTACCCT20550.034.56213410
CCAATAC400.002729696734.1485983
AGAGTTA21250.033.746856
TAAGAGT20950.033.7410964
GTGGTAT2650.033.5392461
ATAAGAG21650.033.2810823
CGGAGGG21500.032.97564728
AAGAGTT21600.032.7293325
CGGCGGA21700.032.6411925
TATCAAC25450.032.6198542
TTCGGCG21800.032.47360623
Produced by FastQC (version 0.11.5)