FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308252
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59410.4541174024576305No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47710.3646850912515326No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42880.32776559867670757No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40350.3084268168517992No Hit
GACTTACAGTCCCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT39210.2997128993496666No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCG36300.27746947835738067No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34690.265162980832439No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33950.2595065782433354No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32540.24872883817490818No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32320.24704720497274227No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA31370.2397856070542984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30880.23604015128583788No Hit
CTGTAAGTCTGTTGACAGTAGTAAGCTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGCGAAATCTGTC30030.2295429320956513No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAA29550.2258739142000165No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29400.22472734610763062No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28760.21983532224678423No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28430.21731287244353537No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27520.21035702601639442No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27400.2094397715424857No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27240.2082167655772741No Hit
GTAGTAAGCTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCA26820.2050063749185937No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26230.20049654042187592No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26230.20049654042187592No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG26040.19904422083818715No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAGCAC25960.19843271785558136No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25370.1939228833588636No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25260.1930820667577806No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC24870.19010098971757736No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24740.18910729737084295No Hit
GCTTACTACTGTCAACAGACTTACAGTCCCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA24650.1884193565154114No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24540.18757853991432843No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT24530.1875021020415027No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24440.1868141611860712No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24350.18612622033063966No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23520.17978187688610453No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22360.17091508363832045No Hit
GGCCAAAAGTGTGAGGGGGACTGTAAGTCTGTTGACAGTAGTAAGCTGCA21920.16755181723398857No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21630.16533511892204256No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21570.1648764916850882No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGAGGGGGA21500.16434142657530812No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20870.15952584058728747No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT20410.15600969843730414No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19590.14974179286559472No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT19590.14974179286559472No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19430.14851878690038312No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19120.14614921284278565No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAGCTTACTACTGTCAAC18650.14255663281997658No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18470.14118075110911352No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17570.1343013425547983No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCGCT17400.133001898716761No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17160.1311673897689436No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGA17160.1311673897689436No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16810.12849206422004322No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16810.12849206422004322No Hit
GTATATTACTGTGCAACTCTGAGGGACTGGGGTGGGGCGGCCTTTGACTA16800.1284156263472175No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCGCTCTCA16650.12726905825483165No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16470.1258931765439686No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16440.12566386292549142No Hit
CTGCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCACTGAACCT16280.12444085696027983No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16230.1240586675961512No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16170.12360004035919686No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15950.12191840715703091No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15930.12176553141137947No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15800.12077183906464503No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15630.11947239522660771No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15420.1178671998972675No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15390.11763788627879032No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT15340.1172556969146617No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15200.11618556669510155No Hit
GATTTCGCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAGCTTACTACTGTCAACAGACTTACAGTCCC15180.11603269094945011No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15070.11519187434836714No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14930.11412174412880698No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14870.11366311689185264No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14840.11343380327337546No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14270.10907684452230917No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14130.10800671430274901No Hit
GCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG14050.10739521132014321No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14030.10724233557449175No Hit
GTGTGAGGGGGACTGTAAGTCTGTTGACAGTAGTAAGCTGCAAAATCTTC13950.10663083259188595No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCGCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAGCTTA13810.10556070237232583No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13800.1054842644995001No Hit
AGTCAGAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG13790.10540782662667436No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13720.10487276151689431No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13710.10479632364406857No Hit
GTCTGCAACCTGAAGATTTTGCAGCTTACTACTGTCAACAGACTTACAGTCCCCCTCACACTTTTGGCCAGGGGA13690.10464344789841713No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG13530.10342044193320553No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCGCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG13390.10235031171364538No Hit
GTGCTAATGCTCTGACTTGCCCGGCAAGAGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA13380.10227387384081967No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13350.10204456022234248No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13320.1018152466038653No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13230.10112730574843379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATT404.073866E-544.65131458
TAGTACG405.570609E-542.345744
TCTGGAG11300.038.17439370
GTATCAA21250.037.1532141
AATGGTA6750.035.39451670
GTGGTAT2300.035.35761
TATCAAC23650.033.2345472
ATCAACG23950.032.2437863
TCAACGC24250.031.7112874
CAACGCA24550.031.3261685
AACGCAG25000.030.8978166
TTGTATA553.6288434E-430.7910163
ACGCAGA26350.029.1862417
CGCAGAG26450.028.947818
CGTTGTC8900.028.59104363
AGAGTAC27650.027.81188611
GCAGAGT27750.027.712729
GCGTTGT9250.027.41582762
GTTGTCT9700.027.0736564
GGCGTTG9400.026.89363761
Produced by FastQC (version 0.11.5)