FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175156
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT7680.43846628148621797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.38422891593779257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.37509420174016306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.3682431660919409No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.2791797026650529No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.2597684349950901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.25691383680833085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.24321176551188656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.23350613167690518No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.2106693461828313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3630.2072438283587202No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.20496014980931282No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.1958254356116833No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.19011623923816484No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.1832652035899427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.1786978464911279No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.17698508757907236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.17184681084290576No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.16670853410673914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.1638539359199799No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.15586106099705407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.14387174861266527No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.13759163260179497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.13245335586562834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2250.12845691840416543No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG2230.12731507912946172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.1256023202174062No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.12217680239329512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.12103496311859142No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.11875128456918405No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.11875128456918405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.11646760601977665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.1147548471077211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.11304208819566558No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1940.11075840964625819No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.11075840964625819No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.10733289182214711No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.10447829363538787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1770.10105277581127681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAATT150.001348252679.376844
ATGGCAG200.00256913667.46487455
ATAAATG250.004037926460.18468562
CTGTATC150.005197943756.4840051
TAAAGAG250.005603033655.40812357
AAATGCG250.00646681553.4372854
GTCTTCT505.657816E-542.19432449
AAGTACG651.5097612E-939.1043131
GTTGGGC450.001416105839.0036553
CGTATGC501.0934482E-437.73502341
ATACGGG603.256355E-837.6560061
TCTCGTA400.002463773634.8497238
GGGGAGT650.002469255134.81904668
ATATCAA501.5095378E-533.8904041
TCGTATG552.216908E-433.46195240
GTAATCT400.003502291932.4380134
CTCTTAT350.003590369832.2765731
CCATGCT350.003595396232.267364
CGTCTTC652.812731E-432.12821648
GTATGCC602.844229E-432.06987842
Produced by FastQC (version 0.11.5)