FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004691948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175156
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12960.7399118500079929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.4259060494644774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.4247642101897737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.34654821987257073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.2746123455662381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4510.2574847564456827No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.22380049784192377No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.22322957820457195No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.21923314074310898No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.2066729087213684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.19068715887551668No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.18440704286464638No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.17926876612847975No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.17584324830436868No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG3070.17527232866701684No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.1638539359199799No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2810.16042841809586883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.16042841809586883No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2610.1490100253488319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.14330082897531343No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.13816255223914684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2410.13759163260179497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.12788599876681359No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.1244604809427025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.12217680239329512No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT2130.12160588275594328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.11989312384388774No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.11760944529448035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.11532576674507297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.11190024892096188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.10790381145949896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.10676197218479526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.10390737399803604No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.1033364543606842No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.10276553472333234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)