FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046852
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36260.34637178894437803No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCATAGCATTGATGGTGTCCCACGCCTGA27110.25896688357093456No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25950.24788604310829035No Hit
GTAATAAACACCCGTGTCGTCGGCTCTCAGGCTGGTCATTTCCAGAGACA25390.24253667185046215No Hit
CTATTACTGTCAGGCGTGGGACACCATCAATGCTATGGTTTTCGGCGGAG25090.23967093724805416No Hit
CTCCTGTGTTGGTCATCTATCAAGATAGTAGGCGGCCCTCAGGAATCCCT24880.2376649230263686No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCATAGCATTGATGGTGTCCCACGCCTGACAGTAATAGT24370.23279317420227502No Hit
CCTATGAACTGACTCAGCCACCGTCAGTGTCCGTGTCCCCAGGACAGACA24350.23260212522878115No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23950.22878114575890385No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23010.21980184400469216No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21550.20585526893963998No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21430.20470897509867678No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC20600.19678044269868136No Hit
ACACAGGAGACTGGCCTGGCTTTTGTTGATACCAGAAAGTATACTTTTCC20080.19181316938784088No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19850.18961610619266142No Hit
GTTTATTACTGTGCGAGAGGCAGGAGGATATCGGGGACCGAGCACTATTT19590.18713246953724116No Hit
CAGTAATAGTCACCCGCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT18660.17824869226977644No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18320.1750008597203807No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18280.174618761773393No Hit
CCCCAGGACAGACAGCCAGCATCGCCTGCTCTGGAGATAAATTGGGGGAA18250.17433218831315217No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17370.1659260334794221No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16730.15981246632761842No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16540.1579975010794267No Hit
GGCTATGGATGCGGGTGACTATTACTGTCAGGCGTGGGACACCATCAATG16520.15780645210593283No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16410.15675568275171659No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16310.15580043788424724No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATT16260.15532281545051257No Hit
CCATCAATGCTATGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA15890.15178840944087607No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15380.1469166606167825No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14950.14280910768666438No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14910.14242700973967667No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14540.13889260373004017No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14430.1378418343758239No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14410.13765078540233003No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14400.1375552609155831No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14330.13688658950835456No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14150.1351671487469098No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGCGGGTGACTAT14140.13507162426016286No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14030.1340208549059466No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13780.13163274273727327No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13430.12828938570113063No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13340.12742966532040823No Hit
GGGCTAGAGTGGGTGGCACTTATATGGTTTGATGGAAGTAATAGACTCTA13290.12695204288667356No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATGCCATAGGTAC13210.1261878469926981No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13070.12485050417824105No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATTCCT13000.12418183277101254No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12820.12246239200956774No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12520.11959665740715975No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12340.11787721664571495No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12210.11663539831800485No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12060.11520253101680086No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12010.11472490858306618No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11940.11405623717583765No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGCATCCGTGG11750.11224127192764594No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGATGGGTTTTC11660.11138155154692354No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT11610.11090392911318889No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11570.11052183116620114No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11530.11013973321921341No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11340.1083247679710217No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10890.10402616606740972No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10830.10345301914692813No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10750.10268882325295266No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGCATCCGTGGCCCCCTATGAACTGACT10650.10173357838548333No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCATAG10630.10154252941198946No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10530.10058728454452014No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10530.10058728454452014No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10530.10058728454452014No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10500.10030071108427935No Hit
AAAGTATACTTTCTGGTATCAACAAAAGCCAGGCCAGTCTCCTGTGTTGGTCATCTATCAAGATAGTAGGCGGCC10480.10010966211078548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)