FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046852
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33140.316568149079335No Hit
CTCCTGTGTTGGTCATCTATCAAGATAGTAGGCGGCCCTCAGGAATCCCT25790.24635765132033946No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCATAGCATTGATGGTGTCCCACGCCTGA25700.24549793093961705No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25320.24186800044323364No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24190.2310737334408302No Hit
CTATTACTGTCAGGCGTGGGACACCATCAATGCTATGGTTTTCGGCGGAG23900.2283035233251692No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCATAGCATTGATGGTGTCCCACGCCTGACAGTAATAGT22850.21827345221674127No Hit
CCTATGAACTGACTCAGCCACCGTCAGTGTCCGTGTCCCCAGGACAGACA22690.21674506042879033No Hit
GTAATAAACACCCGTGTCGTCGGCTCTCAGGCTGGTCATTTCCAGAGACA22460.21454799723361087No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21100.201556667036028No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20810.19878645692036698No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20330.1942012815565142No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC19890.18999820413964916No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19870.1898071551661553No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19370.18503093082880864No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18960.18111442687218443No Hit
GTTTATTACTGTGCGAGAGGCAGGAGGATATCGGGGACCGAGCACTATTT18460.17633820253483778No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18050.17242169857821354No Hit
CAGTAATAGTCACCCGCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT17860.17060673333002183No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17760.16965148846255249No Hit
ACACAGGAGACTGGCCTGGCTTTTGTTGATACCAGAAAGTATACTTTTCC17470.16688127834689145No Hit
CCCCAGGACAGACAGCCAGCATCGCCTGCTCTGGAGATAAATTGGGGGAA17180.1641110682312304No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16940.161818480549304No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATGCCATAGGTAC16760.16009903978785922No Hit
GGCTATGGATGCGGGTGACTATTACTGTCAGGCGTGGGACACCATCAATG16140.1541765216095494No Hit
GGGCTAGAGTGGGTGGCACTTATATGGTTTGATGGAAGTAATAGACTCTA16130.15408099712280246No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15590.1489226748384681No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15410.1472032340770233No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATT15090.14414645050112146No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14860.141949387305942No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14780.14118519141196653No Hit
CCATCAATGCTATGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA14470.13822393232281163No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14410.13765078540233003No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14380.13736421194208925No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14320.13679106502160765No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATTCCT14100.13468952631317513No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13840.13220588965775487No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13720.13105959581679166No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13230.12637889596619198No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGCGGGTGACTAT13120.1253281266119757No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13090.12504155315173493No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13080.12494602866498798No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12940.12360868585053093No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12820.12246239200956774No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12710.12141162265535148No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12600.12036085330113522No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12580.12016980432764135No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11960.11424728614933151No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11840.11310099230836834No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGCATCCGTGG11780.11252784538788674No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11670.11147707603367048No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11460.10947106181198488No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11370.10861134143126248No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11310.1080381945107809No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT11180.10679637618307078No Hit
ATGCTATGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCC10800.10316644568668731No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10770.10287987222644653No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10740.10259329876620572No Hit
GTTTGGTCCCTCCGCCGAAAACCATAGCATTGATGGTGTCCCACGCCTGACAGTAATAGTCACCCGCATCCATAG10650.10173357838548333No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGCATCCGTGGCCCCCTATGAACTGACT10620.10144700492524254No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT10610.10135148043849561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)