FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210261
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT33711.603245490128935No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC5770.27442083886217605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.20403213149371496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.19880053837849151No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.18643495465159968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.14743580597447933No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3100.14743580597447933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.14030181536281097No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG2950.14030181536281097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.1284118310100304No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT2670.12698503288769672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.12555823476536307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.12175343977247327No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT2480.11794864477958347No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.11747304540547225No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.10796105792324777No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.10368066355624676No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.10177826605980186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2110.1003514679374682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)