FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210261
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53832.560151430840717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.22638530207694246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.22590970270283126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.1869105540257109No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3390.16122818782370482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.15980138970137114No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.1426798122333671No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2920.13887501724047732No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT2590.12318023789480692No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.12080224102425081No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.11604624728313857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.11509504853491612No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT2140.10177826605980186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTTT150.002409660368.5829838
ATCTAAT250.00586202654.7876665
GAAGTGA250.00939397548.6303754
ACGTAGG254.8515463E-448.5029751
CCCTGAA800.001191373340.3838870
CAAACAG601.2642701E-436.81881362
CGGGGTA350.00253580834.65323617
GTCAAGG350.00253877334.6449851
AGTACTC350.002547684834.620255
TCATGTC350.002553639734.6037811
TGCATGA500.003817947331.872352
AAGCAAT400.00401252331.55428929
GACAGGT503.965007E-430.314361
ATAACCG503.9816432E-430.2927155
CTCTGTG500.004956962530.21720947
TGCTCTG500.005289309729.81903345
CCAAAAG450.00822459927.2445625
GTATCAA31550.026.999481
CTAAAAC450.00866871526.95251717
TATCGGT450.00869898326.93326813
Produced by FastQC (version 0.11.5)