FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832683
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACTGGTCATCTATCAGGATACCAAGCGGCCCTCAGGGATCCCT80590.9678352986670798No Hit
GTATAGGGGACTGGCCTGGCTTCTGCTGATACCAGGAAGCATAGTTGTCA54140.6501874062518389No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39000.4683655124459128No Hit
GCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAATACCACATCAGTGCTAAAGTCCCACGCCTGA28490.34214701152779625No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27360.3285764210389788No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24900.2990333656385443No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23070.2770562146699284No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22290.2676889044210102No Hit
CCTATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA22070.26504684255593064No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTC19770.23742528669373578No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19470.2338224750595365No Hit
GACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAATACCACATCAGTGCTAAAGTCCCACGCCTGACAGTAATAGT19010.2282981638870975No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18700.2245752585317582No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18520.2224135715512386No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17630.21172523036978055No Hit
GGATCGGTGGCCTCCTATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC17200.20656120036076156No Hit
GACTATTACTGTCAGGCGTGGGACTTTAGCACTGATGTGGTATTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGT16940.20343876361112212No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16540.198635014765523No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15980.19190976638168428No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15870.1905887354491445No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15840.1902284542857246No Hit
CTATTACTGTCAGGCGTGGGACTTTAGCACTGATGTGGTATTCGGCGGAG15800.18974807940116467No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15620.18758639242064507No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15460.18566489288240542No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15440.1854247054401255No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15310.18386348706530578No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15290.18362329962302582No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15000.18014058170996647No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG14510.17425598937410755No Hit
GTGCTAAAGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGG14420.17317514588384775No Hit
CAACTATGCTTCCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTATACTGGTCATCTATCAGGATACCAAGCGGCC14420.17317514588384775No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14340.17221439611472794No Hit
GTAATAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGG14270.17137374006674808No Hit
CAGTAATAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGT13770.16536905400974922No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGTGAC13730.1648886791251893No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13570.16296717958694965No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13230.15888399306819043No Hit
GCACAGGATCGGTGGCCTCCTATGAGTTGACTCAGCCACCCTCAGTGTCC13190.1584036181836305No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13070.15696249352995076No Hit
CTTTAGCACTGATGTGGTATTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT12940.15540127515513105No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTGTGGATGAGGCTGACTATTACTGTCAGGCGTGGGACTTTAGCA12800.15371996305917138No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12590.15119799491523184No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12070.144953121415953No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11740.14099002861833373No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11650.13990918512807396No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11170.134144686513355No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11050.1327035618596753No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCGGTGG10870.1305418748791557No Hit
GAGTGGCTGTGTTCCCAGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCT10820.1299414062734558No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10490.12597831347583655No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10360.12441709510101684No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10360.12441709510101684No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10350.12429700137987684No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10280.12345634533189702No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAATACCACAT10120.12153484579365736No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9910.11901287764971784No Hit
AGCCAGGCCAGTCCCCTATACTGGTCATCTATCAGGATACCAAGCGGCCC9880.11865259648629792No Hit
GATGTGGTATTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC9810.11781194043831807No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCGGTGGCCTCCTATGAGTTGACT9660.1160105346212184No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9580.11504978485209857No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCACCTCTGGGAACACAG9570.11492969113095859No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9140.10976566112193957No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9090.10916519251623967No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8950.10748388042028No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8930.10724369297800003No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAATACCACATCAGTG8920.10712359925686005No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8840.10616284948774023No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8780.10544228716090037No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8710.10460163111292052No Hit
ATACTGGTCATCTATCAGGATACCAAGCGGCCCTCAGGGATCCCTGAGCG8690.10436144367064057No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT8610.10340069390152075No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8390.10075863203644123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAT13450.052.37549270
TCTCGTA800.051.70380438
CCTCTGG24450.046.20282770
TCGTATG1050.046.081540
CGTATGC1100.044.0536441
GTATCAA22650.040.234741
ATGCCGT1350.036.00483344
ATCTCGT1151.8189894E-1235.94613337
TATCAAC25400.035.5917472
TAAGACA1350.035.57808731
CACGAGA1350.035.51185221
AGGGGGG2550.034.8429870
TCAACGC26400.034.5029954
TGAATAC400.002692644534.2435763
ATCAACG26600.034.2435723
CAACGCA27050.033.927095
AATGGTA4950.033.33462570
AACGCAG27650.033.190886
TTATACA1550.033.1389432
CCTATAC20750.032.1807063
Produced by FastQC (version 0.11.5)