FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832683
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACTGGTCATCTATCAGGATACCAAGCGGCCCTCAGGGATCCCT79990.960629675398681No Hit
GTATAGGGGACTGGCCTGGCTTCTGCTGATACCAGGAAGCATAGTTGTCA53530.6428616892623004No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36190.4346191768055791No Hit
GCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAATACCACATCAGTGCTAAAGTCCCACGCCTGA28310.3399853245472767No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27320.3280960461544189No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25580.30719973867606276No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21460.257721125566392No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20970.2518365332305331No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20260.2433098790295947No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19590.2352635997132162No Hit
CCTATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA19530.23454303738637633No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19020.22841825760823745No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18710.2246953522528982No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTC18170.21821029131133934No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17420.20920326222584104No Hit
GGATCGGTGGCCTCCTATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC16850.20235792012086232No Hit
GACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAATACCACATCAGTGCTAAAGTCCCACGCCTGACAGTAATAGT16720.2007967017460426No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16420.1971938901118433No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15930.19130929777598438No Hit
GTGCTAAAGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGG15670.18818686102634496No Hit
GACTATTACTGTCAGGCGTGGGACTTTAGCACTGATGTGGTATTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGT15480.1859050803246854No Hit
CTATTACTGTCAGGCGTGGGACTTTAGCACTGATGTGGTATTCGGCGGAG15470.1857849866035454No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14990.18002048798882647No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14890.1788195507774267No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14740.17701814496032703No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG14720.1767779575180471No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14650.17593730147006723No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14230.17089336518218817No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13850.16632980377886902No Hit
GTAATAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGG13770.16536905400974922No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13730.1648886791251893No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGTGAC13610.16344755447150958No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13510.16224661726010978No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13190.1584036181836305No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12940.15540127515513105No Hit
CTTTAGCACTGATGTGGTATTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT12690.1523989321266316No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12680.15227883840549164No Hit
CAACTATGCTTCCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTATACTGGTCATCTATCAGGATACCAAGCGGCC12530.150477432588392No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12330.14807555816559242No Hit
CAGTAATAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGT12150.14591387118507285No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11940.1433919030411333No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11940.1433919030411333No Hit
GCACAGGATCGGTGGCCTCCTATGAGTTGACTCAGCCACCCTCAGTGTCC11870.14255124699315344No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11650.13990918512807396No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11140.1337844053499351No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCGGTGG11080.13306384302309524No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10910.1310222497637156No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10760.12922084394661595No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10710.12862037534091605No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTGTGGATGAGGCTGACTATTACTGTCAGGCGTGGGACTTTAGCA10640.12777971929293622No Hit
GAGTGGCTGTGTTCCCAGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCT10520.12633859463925648No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10300.12369653277417697No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10240.12297597044733712No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10070.12093437718795749No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10000.12009372113997764No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAATACCACAT9990.11997362741883764No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9930.11925306509199779No Hit
GATGTGGTATTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC9910.11901287764971784No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCGGTGGCCTCCTATGAGTTGACT9790.11757175299603811No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9380.11264791042929902No Hit
AGCCAGGCCAGTCCCCTATACTGGTCATCTATCAGGATACCAAGCGGCCC8890.10676331809344013No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAATACCACATCAGTG8810.1058025683243203No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8770.10532219343976038No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCACCTCTGGGAACACAG8750.10508200599748042No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8530.10243994413240093No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8440.10135910064214113No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8430.10123900692100116No Hit
ATACTGGTCATCTATCAGGATACCAAGCGGCCCTCAGGGATCCCTGAGCG8370.10051844459416127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC200.00763613751.267173
AGGTGAT13150.049.77488370
CCTCTGG23750.045.47328670
CGGTACC602.7633905E-740.51465645
CCTACGT350.001378118439.24935534
TCCTACG350.001382213239.22567433
CGATTAA350.001404077239.1006424
GTTATAC350.001405738339.0912321
GTATCAA22250.036.2801781
ATTAGAG853.252353E-936.188593
ATAGGTA501.9663508E-434.1781126
TATCAAC24300.033.0608522
ATCAACG24900.032.256453
GCGGTAC652.2301721E-532.0320944
TCAACGC25250.031.9466114
CAACGCA25350.031.820595
TTAACGG652.3813327E-531.72630135
AACGCAG25600.031.6414556
AATGGTA4800.031.36336570
ACGGGTA2300.031.21360416
Produced by FastQC (version 0.11.5)