FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388900
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73690.530563755489956No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60860.43818849449204406No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49490.35632514939880483No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48280.34761321909424725No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47890.3448052415580675No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTT46100.33191734466124273No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA45360.3265893872849017No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43870.31586147310821516No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43260.31146950824393405No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA42680.3072935416516668No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38500.2771977824177407No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35460.25530995752033986No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35240.2537259701922384No Hit
CCCCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTC34840.25084599323205414No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34660.24955000359997123No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34030.24501403988768092No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA33740.24292605659154728No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32830.23637410900712794No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32820.23630210958312334No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC32650.235078119375045No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32440.2335661314709482No Hit
GTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTG32220.23198214414284685No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31930.2298941608467132No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31500.2267981856145151No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31280.2252141982864137No Hit
CTCTACAAGCTCCCCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT30860.2221902224782202No Hit
GTACTGGGGAGCTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCT30560.22003023975808192No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAAACCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA30010.21607027143782848No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAGCT29020.2089423284613723No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCGAAAGTGTACTGGGGAG28910.20815033479732165No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28680.20649434804521563No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28350.20411836705306355No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCGAAAGTG28270.2035423716610267No Hit
AAAGTGTACTGGGGAGCTTGTAGAGCTTGCATGCAGTAATAAACCCCAAC28000.2015983872129023No Hit
CTCCTACATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT27640.1990064079487364No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG27530.1982144142846857No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCC27390.1972064223486212No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG26880.1935344517243862No Hit
ATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA25930.18669450644394844No Hit
ATCCATTACTATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC25750.1853985168118655No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG25600.1843185254517964No Hit
GCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC25530.18381452948376412No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25460.18331053351573187No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT25430.18309453524371805No Hit
GTAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA25240.1817265461876305No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAGCACAATATCC25000.1799985600115199No Hit
GTTTATTACTGCATGCAAGCTCTACAAGCTCCCCAGTACACTTTCGGCCA24440.17596659226726186No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGA24080.17337461300309598No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23660.17035063719490243No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23530.16941464468284254No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23130.1665346677226582No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA22900.16487868097055225No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT22800.16415868673050615No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22720.16358269133846928No Hit
CTACAAGCTCCCCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT22720.16358269133846928No Hit
CAGTAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA22330.16077471380228958No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA22200.1598387212902297No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTCCACTCTCC22150.15947872417020664No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22050.15875872993016055No Hit
CACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGC21340.1536467708258334No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21340.1536467708258334No Hit
TTATTACTGCATGCAAGCTCTACAAGCTCCCCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAA21300.15335877312981494No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21270.15314277485780114No Hit
GGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACT20990.1511267909856721No Hit
GTGTACTGGGGAGCTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATC20890.15040679674562601No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20300.14615883072935418No Hit
CTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT20300.14615883072935418No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20050.14435884512923897No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20020.14414284685722514No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTCCACT20020.14414284685722514No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20010.14407084743322054No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19950.14363885088919287No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19790.14248686010511916No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG19740.1421268629850961No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19500.14039887680898552No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19010.13687090503275975No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGT18970.1365829073367413No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18940.13636690906472748No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18800.13535891712866296No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18780.13521491828065374No Hit
GCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCGAAAGTGTACTG18580.1337749298005616No Hit
GGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT18490.1331269349845201No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18410.13255093959248326No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18250.13139894880840952No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTC18220.1311829505363957No Hit
CTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA18220.1311829505363957No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18170.13082295341637268No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18130.13053495572035423No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAA18060.13003095975232198No Hit
ACATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGC17940.1291669666642667No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17470.12578299373605012No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17320.124703002375981No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCCTCCGGGGTCCCTGA17300.12455900352797177No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17260.12427100583195334No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCCTCCGGGGTCCCTGACAGGTT17250.12419900640794873No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA17200.12383900928792571No Hit
GTATTACTGTGCGAGAGACGCCGATGGTGGCCGACGTAGATACTACTATT17040.12268701850385198No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16850.12131902944776442No Hit
CTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCCTCCGGGGTCCCT16820.1211030311757506No Hit
CCTACATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAG16170.11642306861545108No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16060.11563107495140039No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15880.11433508531931745No Hit
GGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATT15810.11383108935128518No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15710.11311109511123911No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15600.11231910144718842No Hit
CCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGG15290.11008711930304557No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15280.11001511987904096No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15260.10987112103103176No Hit
GTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCCTCCGGGGTCC15120.10886312909496725No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCC15110.10879112967096263No Hit
GGCCGAAAGTGTACTGGGGAGCTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTG15100.108719130246958No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15090.10864713082295342No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15000.10799913600691195No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14980.10785513715890271No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA14910.10735114119087047No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14870.10706314349485203No Hit
AGTCAGAGCCTCCTACATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG14810.1066311469508244No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14800.10655914752681979No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14740.10612715098279213No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT14630.10533515731874145No Hit
CTGTATTACTGTGCGAGAGACGCCGATGGTGGCCGACGTAGATACTACTA14490.10432716538267693No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14380.10353517171862625No Hit
GGTTTATTACTGCATGCAAGCTCTACAAGCTCCCCAGTACACTTTCGGCC13990.10072719418244654No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG13950.1004391964864281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)