FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388900
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72470.5217798257613939No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51490.3707250341997264No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51470.3705810353517172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47790.3440852473180214No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA46160.3323493412052703No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43170.31082151342789255No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43100.3103175174598603No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTT40970.29498164014687883No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39600.28511771905824757No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA38160.274749802001584No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35550.2559579523363813No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34730.25005399956800345No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34170.2460220318237454No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33710.24271005831953346No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32220.23198214414284685No Hit
CCCCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTC31700.22823817409460723No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT31670.2280221758225934No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC31260.2250701994384045No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31120.22406220750233996No Hit
GTACTGGGGAGCTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCT30920.22262221902224782No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30820.22190222478220173No Hit
GTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTG30760.2214702282381741No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30710.22111023111815106No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA30680.22089423284613724No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30380.21873425012599898No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30360.21859025127798978No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCGAAAGTG29480.21225430196558426No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29220.21038231694146445No Hit
CTCTACAAGCTCCCCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT28860.2077903376772986No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAAACCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA28200.20303837569299446No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCGAAAGTGTACTGGGGAG27840.20044639642882855No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAGCT27820.20030239758081933No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG27420.19742242062063503No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27170.19562243502051985No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG26720.19238246094031247No Hit
CTCCTACATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT26710.19231046151630787No Hit
AAAGTGTACTGGGGAGCTTGTAGAGCTTGCATGCAGTAATAAACCCCAAC26460.1905104759161927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26330.18957448340413277No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCC25190.18136654906760746No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24520.17654258765929873No Hit
GTTTATTACTGCATGCAAGCTCTACAAGCTCCCCAGTACACTTTCGGCCA24370.1754625962992296No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24280.17481460148318811No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAGCACAATATCC24260.17467060263517892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24010.17287061703506373No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24010.17287061703506373No Hit
ATCCATTACTATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC23570.16970264237886096No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT23560.16963064295485636No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23430.16869465044279647No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA23370.1682626538987688No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23040.16588667290661674No Hit
GCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC22970.1653826769385845No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA22590.1626466988264094No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22440.16156670746634028No Hit
GTAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA22410.16135070919432645No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22160.15955072359421124No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTCCACTCTCC22010.15847073223414213No Hit
ATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA21900.15767873857009143No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21430.15429476564187486No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21030.15141478868169056No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGA21030.15141478868169056No Hit
GTGTACTGGGGAGCTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATC20680.14889480884152925No Hit
CTACAAGCTCCCCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT20510.14767081863345094No Hit
CACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGC20450.1472388220894233No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTCCACT20170.14522283821729426No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA20070.1445028439772482No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20020.14414284685722514No Hit
CAGTAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA20010.14407084743322054No Hit
TTATTACTGCATGCAAGCTCTACAAGCTCCCCAGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAA19990.14392684858521132No Hit
GGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACT19900.14327885376916985No Hit
CTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT19880.14313485492116063No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19780.14241486068111456No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19580.1409748722010224No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG19510.14047087623299015No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19440.13996688026495788No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19200.1382388940888473No Hit
GGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT19170.13802289581683347No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19090.1374469004247966No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAA18660.13435092519259845No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18600.13391892864857083No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18430.1326949384404925No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18340.132046943624451No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18270.13154294765641875No Hit
CTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA18260.13147094823241415No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17970.12938296493628051No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17830.128374973000216No Hit
GCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCGAAAGTGTACTG17820.1283029735762114No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17790.12808697530419755No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17650.12707898336813306No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17520.12614299085607317No Hit
CTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCCTCCGGGGTCCCT17220.12398300813593491No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTC17180.12369501043991647No Hit
ACATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGC17160.12355101159190728No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGT17040.12268701850385198No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17020.12254301965584276No Hit
GGCCGAAAGTGTACTGGGGAGCTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTG16980.12225502195982432No Hit
GGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATT16200.1166390668874649No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16150.11627906976744186No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15870.11426308589531284No Hit
GTATTACTGTGCGAGAGACGCCGATGGTGGCCGACGTAGATACTACTATT15660.11275109799121608No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15580.1121751025991792No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT15550.1119591043271654No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCCTCCGGGGTCCCTGACAGGTT15390.11080711354309164No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15210.10951112391100871No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15190.10936712506299949No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15190.10936712506299949No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15180.10929512563899489No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15170.10922312621499028No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15120.10886312909496725No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCCTCCGGGGTCCCTGA15080.10857513139894881No Hit
GTCTCCACAGCTCCTGATCTATTTGGTTTCTACTCGGGCCTCCGGGGTCC15050.10835913312693499No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14980.10785513715890271No Hit
GGTGTATCCATTACTATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGG14790.10648714810281518No Hit
GGTTTATTACTGCATGCAAGCTCTACAAGCTCCCCAGTACACTTTCGGCC14510.10447116423068616No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14450.1040391676866585No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14450.1040391676866585No Hit
GGGTCTCTGGATCCAATGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG14370.10346317229462164No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCC14290.10288717690258478No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14270.10274317805457557No Hit
GTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGC13940.10036719706242352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.039.6411671
GTATCAA32850.039.1593061
ATTCACT3100.034.8361552
TAATCGT601.3732879E-534.38681838
TCTACTA400.00289297533.7475242
TATCAAC37750.033.3452342
TCTGGAG10500.033.26670570
ATCAACG39700.031.86593
AATCGTT652.3337609E-531.82228739
TTAATCG652.407546E-531.67771537
TCAACGC39900.031.6204834
CAACGCA40400.031.3126415
AACGCAG40650.031.1189486
CGCAGAG42250.029.940488
AGAGTAC42000.029.87882211
ACGCAGA42850.029.5999647
CAGAGTA42550.029.56869510
ACTTTAT3800.029.2943956
CGAACCT853.6185556E-629.15888453
ACGAACC853.6818856E-629.09399652
Produced by FastQC (version 0.11.5)