FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400781
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATATTACTCCTATGATGACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCAT117850.8413163799337655No Hit
ATATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTC97410.6953977816660848No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT86550.6178696027430413No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC85880.6130865567137189No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT83700.5975238099317453No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCTTATTGGTGGGACTGTAGAAAACACT80950.5778919045875123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC63590.45396104030537254No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61410.4383982935233987No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52820.3770753601026856No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA51940.370793150392531No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49890.3561584573177392No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47680.3403815442956465No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG47450.33873960312140156No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG46360.33095822973041467No Hit
ATATTACTCCTATGATGACTTTCGGCCCTGGGACCAAACTGGATATCAAA45100.32196324764542067No Hit
GTTTTCTACAGTCCCACCAATAAGAATTACTTAGCTTGGTACCAGCAGAA44490.31760853409633627No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG43720.312111600599951No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42000.2998327361664671No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41650.2973341300317466No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTA41420.29569218885750165No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40760.29098053157488574No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40710.2906235878413542No Hit
GTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC38620.27570333977973716No Hit
GTAGAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38490.27477528607255525No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38430.2743469535923174No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37380.26685113518815573No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC36370.2596408717708193No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36270.25892698430375627No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA34470.24607700989662196No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACGCTCAC33370.23822424775892878No Hit
GTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA33340.23801008151880984No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCA32800.23415508919666958No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACAGTCCCAC32040.22872954444699065No Hit
GTTTATTACTGTCAGCAGTATTATATTACTCCTATGATGACTTTCGGCCC30890.22051983857576596No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30680.21902067489493363No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30030.214380406359024No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA30030.214380406359024No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT29750.21238152145124758No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAGTCATCATAGGAGTAATATAATACTG29400.209882915316527No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28980.20688458795486234No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC28820.2057423680075615No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCTTATTGGTGGGACTG28800.2055995905141489No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA28310.20210154192554008No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28280.2018873756854212No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27710.19781821712316203No Hit
GAATTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGC26800.19132184117288856No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATATTACTCCTATGATGA26710.19067934245253182No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25620.18289796906154496No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25380.1811846391405937No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACGCTCACCATCAGCAGCC24890.17768659055198494No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24840.17732964681845342No Hit
GAGTAATATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAAT24730.1765443706046841No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACAGTCCCACCAATAAGAATTA24280.1733318770029005No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24190.1726893782825438No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23520.1679063322532216No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23520.1679063322532216No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23120.16505078238496954No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC23110.16497939363826322No Hit
GTCTATTACTGTGCGAATAATCCGGTCTCATTTGAATATTGTTCCGGTGACTGCTACTACTTTGAATCTTGGGGC22870.16326606371731198No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT22740.16233801001013007No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG22710.16212384377001116No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22700.16205245502330484No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22650.16169551128977336No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22640.16162412254306704No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22150.15812607395445827No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22120.15791190771433936No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21630.15441385912573058No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21550.15384274915208018No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21520.15362858291196124No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCC21180.151201365523947No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21060.15034470056347138No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20510.1464183194946248No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20450.14598998701438698No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20410.14570443202756175No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG20370.14541887704073655No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20030.1429916596527223No Hit
CCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAG20000.1427774934126034No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT19750.14099277474494584No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19490.13913666733058203No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18690.13342556759407787No Hit
CTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCA18380.1312125164461825No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18320.1307841839659447No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCA18100.1292136315384061No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18070.12899946529828718No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC17970.12828557783122416No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17900.12778585660428005No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17630.1258583604432099No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGTTGA17500.12493030673602797No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17430.12443058550908387No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17390.12414503052225867No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17380.12407364177555236No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGATGTCTTTTT17250.12314558806837043No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC17220.12293142182825154No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG17190.12271725558813261No Hit
GTCTGGGACAGATTTCACGCTCACCATCAGCAGCCTGCAGGCTGAAGATG16980.12121809190730028No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16850.12029003820011835No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16850.12029003820011835No Hit
CTACGGGGACATCGTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG16820.12007587195999946No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16710.11929059574623015No Hit
AAGTAATTCTTATTGGTGGGACTGTAGAAAACACTCTGGCTGGACTTGCA16620.11864809702587342No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16600.11850531953246082No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16320.11650643462468437No Hit
CTACAGTCCCACCAATAAGAATTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTA16220.11579254715762136No Hit
GCTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT16180.11550699217079616No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15910.113579496009726No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15820.1129369972893693No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACGCTCACCATCAG15400.10993866992770461No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA15360.10965311494087941No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTC15330.10943894870076051No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATATTACTCCTA15160.10822534000675338No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG15060.10751145253969036No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA15020.10722589755286516No Hit
CTCCTATGATGACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACT14980.10694034256603996No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACAGTCCCACCAATAA14950.10672617632592103No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT14820.10579812261873912No Hit
GATTTCACGCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATATTACT14790.10558395637862023No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT14790.10558395637862023No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT14640.10451312517802569No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT14580.10408479269778786No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC14560.10394201520437527No Hit
GCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT14430.10301396149719334No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT14310.10215729653671773No Hit
CATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG14250.10172896405647991No Hit
GGACCAAACTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14240.10165757530977361No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14220.10151479781636101No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14200.10137202032294841No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACGCTCACCATCAGCAGC14180.10122924282953581No Hit
GTCTGGAGACTGGGTCAACACGATGTCCCCGTAGGCACCAGAGATCCAGAGCAACAGAGAAATGAAGACCTGGGT14130.1008722990960043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2600.052.1343731
CGTATGC1100.050.2396841
CGCGTAG1100.049.419528
TCGTATG1150.047.96457340
GACGCGT1150.047.21842226
TGGTATC3100.043.7146762
ACGCGTA1250.043.4642627
AGACGCG1250.043.42231425
CGAGACG1250.043.39439423
ACGAGAC1250.043.38354522
GGTATCA9200.042.7275161
CCGTCTT1300.040.2402447
GAGACGC1400.038.74914624
TGTATAA350.001473815338.718712
TCCGAGC1400.038.71594613
GTATCAA24000.038.688051
ATGCCGT1450.038.3010544
ATCTCGT1350.038.09056537
CACGAGA1350.037.65797821
GTAGTAA1500.036.33854331
Produced by FastQC (version 0.11.5)