FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400781
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATATTACTCCTATGATGACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCAT124270.8871479553192112No Hit
ATATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTC109390.7809215002202343No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82520.5890999378204016No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCTTATTGGTGGGACTGTAGAAAACACT73040.5214234059428277No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT71090.5075026003350988No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67910.48480097888249485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC64590.46109991497600267No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC63050.45010604798323217No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51100.3647964956692017No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49140.35080430131476653No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA48510.34630681027226956No Hit
ATATTACTCCTATGATGACTTTCGGCCCTGGGACCAAACTGGATATCAAA43990.3140390967610212No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT43370.3096129944652305No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42840.3058293908897965No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCA42470.3031880072616633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC41920.2992616261928167No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT41910.29919023744611045No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41400.295549411364089No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39160.2795583321018774No Hit
GTTTTCTACAGTCCCACCAATAAGAATTACTTAGCTTGGTACCAGCAGAA38380.2739900098587859No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38200.2727050124180725No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36360.259569483024113No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT36350.25949809427740667No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC36060.2574278206229239No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTA35380.25257338584689537No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35080.25043172344570636No Hit
GTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC34960.24957505848523073No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34170.24393534749543289No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT33640.2401517439199989No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA33630.24008035517329263No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32210.22994315314099778No Hit
GTTTATTACTGTCAGCAGTATTATATTACTCCTATGATGACTTTCGGCCC31530.22508871836496927No Hit
GTAGAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA31110.22209039100330458No Hit
GTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA30630.2186637311614021No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAGTCATCATAGGAGTAATATAATACTG30190.21552262630632485No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30100.2148801275859681No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACGCTCAC29640.21159624523747825No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCTTATTGGTGGGACTG28670.20467153680696695No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27140.1937490585609028No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27120.1936062810674902No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA26980.19260683861360198No Hit
GAGTAATATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAAT26810.19139322991959484No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACAGTCCCAC26650.19025100997229402No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26610.18996545498546882No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA25930.1851110202094403No Hit
GCTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT25540.18232685908789453No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC25520.18218408159448193No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA25460.1817557491142441No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25380.1811846391405937No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT25320.1807563066603559No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC25240.1801851966867055No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24200.1727607670292501No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23700.16919132969393502No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATATTACTCCTATGATGA23670.16897716345381614No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG23580.1683346647334594No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23550.1681204984933405No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23170.16540772611850105No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22830.16298050873048678No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22780.16262356499695527No Hit
GTCTATTACTGTGCGAATAATCCGGTCTCATTTGAATATTGTTCCGGTGACTGCTACTACTTTGAATCTTGGGGC22650.16169551128977336No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCA22300.15919690515505278No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22210.15855440643469607No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT22180.15834024019457715No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22160.15819746270116455No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACGCTCACCATCAGCAGCC22030.15726940899398265No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22020.15719802024727633No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21780.15548469032632511No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21690.15484219160596838No Hit
GAATTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGC21010.14998775682993987No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20840.14877414813593276No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20620.14720359570839409No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACAGTCCCACCAATAAGAATTA20200.14420526834672945No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCC20120.14363415837307902No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19060.13606695122221102No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT18990.13556722999526694No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18970.13542445250185434No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18710.13356834508749046No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18530.13228334764677704No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18470.13185501516653925No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18370.13114112769947622No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18300.1306414064725321No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18050.12885668780487455No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17980.12835696657793044No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17730.1265722479102729No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17690.12628669292344769No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17590.1255728054563847No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGTTGA17520.12507308422944058No Hit
CCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAG17510.12500169548273427No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17390.12414503052225867No Hit
GTCTGGGACAGATTTCACGCTCACCATCAGCAGCCTGCAGGCTGAAGATG17330.12371669804202085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17320.12364530929531453No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGATGTCTTTTT17010.12143225814741918No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16980.12121809190730028No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATATTACTCCTA16970.12114670316059398No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16950.12100392566718138No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16770.11971892822646794No Hit
CTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCA16710.11929059574623015No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16350.11672060086480329No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG16020.11436477222349532No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15780.11265144230254408No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15760.11250866480913148No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15570.11115227862171174No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15540.11093811238159285No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15290.10915339371393529No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15060.10751145253969036No Hit
AAGTAATTCTTATTGGTGGGACTGTAGAAAACACTCTGGCTGGACTTGCA14970.10686895381933365No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG14770.1054411788852076No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14690.1048700689115572No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT14600.10422757019120046No Hit
GTCTGGAGACTGGGTCAACACGATGTCCCCGTAGGCACCAGAGATCCAGAGCAACAGAGAAATGAAGACCTGGGT14560.10394201520437527No Hit
CTACGGGGACATCGTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14520.10365646021755007No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT14500.10351368272413747No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG14350.10244285152354293No Hit
GGACCAAACTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14320.10222868528342403No Hit
GATTTCACGCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATATTACT14140.1009436878427106No Hit
CTCCTATGATGACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACT14010.10001563413552868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)