FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598123
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25010.4181414190726656No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23630.39506924161083923No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17800.29759765131921023No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17570.2937522884089059No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16810.2810458718357261No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16600.2775348883089264No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15730.2629893851264707No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT14660.24510008810896758No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14650.2449328984172152No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13240.22135915188013167No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13200.22069039311312222No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12890.2155075126687989No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG12550.2098230631492185No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12130.2028010960956191No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12080.2019651476368573No Hit
GTTCAGTGGAAGTCGGTCTGAGACAGATTATACTTTCACCATTAGCAGCC11640.1946088011997532No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11310.1890915413719252No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10990.18374147123584947No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10750.17972891863379273No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10400.17387727942245992No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10310.17237257219668864No Hit
GTCTGAGACAGATTATACTTTCACCATTAGCAGCCTGCAGCCTGAAGATG10210.170700675279165No Hit
GTCTCCATCCTCCCTGTCTGCATCTCTAGGAGACAGAATCACCATCACTTGCCAGGCGAGTCGGGACATTAGAAC9930.16601936391009875No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9730.16267557007505146No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9670.16167243192453726No Hit
ATATTATTGTCAACAGTATGATAAACTTCCGCTCACTTTCGGCGGAGGGA9670.16167243192453726No Hit
GATTATACTTTCACCATTAGCAGCCTGCAGCCTGAAGATGTTGCGACATATTATTGTCAACAGTATGATAAACTT9560.15983334531526125No Hit
GTATAATCTGTCTCAGACCGACTTCCACTGAACCGTGATGGGACCCCAGT9500.15883020716474705No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9420.15749268963072813No Hit
CATTAGAACCTCTTTAAATTGGATTCAGCATCAACCTGGGAAAGCCCCTAAACTCCTGATCTACGATACTTCCAG9030.15097229165238588No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8870.14829725658434803No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8850.1479628772008433No Hit
AGATTATACTTTCACCATTAGCAGCCTGCAGCCTGAAGATGTTGCGACAT8460.14144247922250106No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8410.14060653076373922No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8400.14043934107198686No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC8290.13860025446271085No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8170.13659397816168248No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8130.135925219394673No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8080.1350892709359112No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7980.13341737401838752No Hit
GTCTCAGACCGACTTCCACTGAACCGTGATGGGACCCCAGTTTCCAAGCT7900.1320798564843686No Hit
CAATAATATGTCGCAACATCTTCAGGCTGCAGGCTGCTAATGGTGAAAGTATAATCTGTCTCAGACCGACTTCCA7860.13141109771735915No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7730.1292376317245784No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT7630.12756573480705474No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7610.12723135542355002No Hit
GTATGATAAACTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT7570.12656259665654054No Hit
CCACTGAACCGTGATGGGACCCCAGTTTCCAAGCTGGAAGTATCGTAGAT7500.12539226881427398No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT7490.12522507912252162No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7470.12489069973901688No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7420.12405475128025507No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7420.12405475128025507No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7380.1233859925132456No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7190.12020938836995065No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7140.11937343991118884No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7020.11736716361016046No Hit
ACCTGGGAAAGCCCCTAAACTCCTGATCTACGATACTTCCAGCTTGGAAA6990.11686559453490336No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6910.11552807700088442No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6890.1151936976173797No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6850.11452493885037024No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6830.11419055946686552No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6820.11402336977511314No Hit
CCATTAGCAGCCTGCAGCCTGAAGATGTTGCGACATATTATTGTCAACAG6790.11352180069985605No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6790.11352180069985605No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6420.10733578210501854No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6330.10583107487924726No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6260.10466074703698068No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6100.10198571196894284No Hit
GTGGAAGTCGGTCTGAGACAGATTATACTTTCACCATTAGCAGCCTGCAG6100.10198571196894284No Hit
GCATCAACCTGGGAAAGCCCCTAAACTCCTGATCTACGATACTTCCAGCTTGGAAACTGGGGTCCCATCACGGTT6070.10148414289368575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT205.7405443E-498.4430370
ATACCCC200.00713798652.1474244
CGTATGC2700.043.5111641
TCGTATG2700.043.4291240
GGTATCA7950.042.3393971
GCATAGA650.001196341740.38688770
CCCACGA2950.039.91200619
ATGCCGT2950.038.889644
CGAGACG2950.038.79761523
TATACAC3050.038.6066443
TATGCGG350.001524606838.4490665
GTATGCC3100.037.97835542
CCGTCTT3050.037.90271447
GCCGTCT3050.037.81382846
CTACATC2800.037.5901233
TCTCCGA3050.037.49732611
GCCCACG3150.037.37791418
ACGAGAC3200.036.8061922
CGAGCCC3200.036.7938815
CGTCTTC3150.036.7827248
Produced by FastQC (version 0.11.5)