FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326871
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14580.4460475233348936No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT12270.3753774424773076No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG9960.30470736161972156No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT9820.30042432641623146No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC9190.2811506680005262No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT8710.26646597587427456No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC8410.2572880432953673No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC8280.2533109391778408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7730.23648472944984414No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCC6250.1912069287272349No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG5880.1798874785465826No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC5880.1798874785465826No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG5760.17621630551501968No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC5710.1746866500852018No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG5590.1710154770536389No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC5370.16428499316244022No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT5180.1584723025291323No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG5110.15633078492738725No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA5020.15357740515371507No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT4870.14898843886426144No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA4850.14837657669233428No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC4490.13736305759764555No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT4340.1327740913081919No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA4230.1294088493625926No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAA4230.1294088493625926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.129102918276629No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT4000.12237243438543033No Hit
CCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGAT3980.1217605722135032No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC3890.119007192439831No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA3880.11870126135386744No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA3860.11808939918194028No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA3850.11778346809597671No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTG3810.11655974375212239No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA3810.11655974375212239No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT3800.11625381266615882No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT3800.11625381266615882No Hit
GTATCAACGCAGAGTACGGGGTACAGTGAAACTGCGAATGGCTCATTAAA3640.1113589152907416No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA3540.10829960443110584No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAG3520.10768774225917871No Hit
AAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAAC3430.10493436248550651No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCG3380.10340470705568862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2100.060.0221970
GCGATTA200.00865533249.66085415
TCGTATG2150.046.76674740
ATGTCGT306.560584E-445.6297737
CTCGTAT1950.044.22443439
TGCCGTC2600.043.4456945
CGTATGC2350.042.9863141
TATACAC2550.042.8446583
CGTCTTC2700.042.2779248
CCGTCTT2700.042.08370247
GCCGTCT2650.041.4358346
TCTCGTA2100.040.87660238
ACGAGAC2600.040.80354322
CCCTAAG2700.040.78257428
TTATACA2700.040.46442
ATGCCGT2650.039.8448644
CCCACGA2750.039.75909419
GTCGTAT350.001347270439.42292439
ATCTCGT2300.038.68610837
CTTATAC2850.038.3464281
Produced by FastQC (version 0.11.5)