FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976784
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48510.49662975642516666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42190.4319276319022424No Hit
GACTTTACTCTCACCATCAACAGCCTGCAGACTGAAGATTTTGCCACTTATTATTGTCAACATTATGCTGTTTAT36600.37469901226883323No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31840.32596766531802324No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30040.3075398450425068No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29620.303240020311553No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC28250.28921440154629885No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27250.27897672361545645No Hit
ACATTATGCTGTTTATCCGTACACTTTTGGCCGGGGGACCAAGGTGGAAATCAGTCGAACTGTGGCTGCACCAT26370.2699675670363151No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24910.2550205572572851No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24620.2520516306573408No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24430.25010647185048074No Hit
GAGTAAAGTCTGTCCCATATCCACTGCCGCTGAACCTTGACGGGACCCCA24240.2481613130436207No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23700.24263296696096578No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21580.22092908974757983No Hit
GTCTCCAACCTCACTGTCTGCATTTGTAGGACAGACAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAAGAC21230.21734590247178498No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21140.21642451145800914No Hit
GTGCAGCCACAGTTCGACTGATTTCCACCTTGGTCCCCCGGCCAAAAGTG21030.21529836688561646No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20950.2144793526511491No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20700.2119199331684385No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20690.21181755638913002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19870.20342266048583926No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19630.20096561778243704No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT19370.19830382152041803No Hit
GCATAATGTTGACAATAATAAGTGGCAAAATCTTCAGTCTGCAGGCTGTT19070.1952325181411653No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18960.19410637356877264No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT18840.19287785221707152No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGACTGATTTCCACCTTGGTCC18830.1927754754377631No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGACAT18470.18908991138265982No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCT18410.18847565070680927No Hit
CTTTTGGCCGGGGGACCAAGGTGGAAATCAGTCGAACTGTGGCTGCACCA18010.18438057953447232No Hit
GGATATGGGACAGACTTTACTCTCACCATCAACAGCCTGCAGACTGAAGA17720.181411652934528No Hit
ATTAAGACCTTTGTAGCCTGGTTTCAGCAGAGACCCGGGAAAGCCCCCAG17490.17905698701043424No Hit
GTTTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCAACCTCA16430.16820504840374126No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG16200.1658503824796475No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC15940.16318858621762847No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15580.15950302216252518No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCAACCTCA15480.15847925436944094No Hit
ATATGGGACAGACTTTACTCTCACCATCAACAGCCTGCAGACTGAAGATT15070.15428180641779554No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14980.15336041540401973No Hit
AGTCAGGATATTAAGACCTTTGTAGCCTGGTTTCAGCAGAGACCCGGGAA14970.1532580386247113No Hit
CACCATCAACAGCCTGCAGACTGAAGATTTTGCCACTTATTATTGTCAAC14830.15182476371439338No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14440.1478320693213648No Hit
GGATATTAAGACCTTTGTAGCCTGGTTTCAGCAGAGACCCGGGAAAGCCC14390.14732018542482267No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC14290.14629641763173845No Hit
ACCTCACTGTCTGCATTTGTAGGACAGACAGTCACCATCACTTGTCGGGC14200.14537502661796262No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14040.14373699814902782No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13950.14281560713525202No Hit
GGCCAAAAGTGTACGGATAAACAGCATAATGTTGACAATAATAAGTGGCA13750.14076807154908352No Hit
CAATAATACACCGCCGTGTCCTCGACTCTCAGGCTATTCATTTCCAGATA13690.14015381087323298No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13670.13994905731461613No Hit
GTTTATCCGTACACTTTTGGCCGGGGGACCAAGGTGGAAATCAGTCGAACTGTGGCTGCACCATCTGTCTTCAT13330.1364682468181297No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13100.13411358089403594No Hit
GATCTATGATGCAAGTAGTTTGCAGAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATATGGGACAGACTTTA12910.13216842208717586No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12760.1306327703975495No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12590.1288923651493063No Hit
GGTCTTAATATCCTGACTCGCCCGACAAGTGATGGTGACTGTCTGTCCTACAAATGCAGACAGTGAGGTTGGAGA12570.12868761159068945No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12540.12838048125276416No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12350.1264353224459041No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12070.12356877262526822No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12010.12295451194941767No Hit
GATTTCCACCTTGGTCCCCCGGCCAAAAGTGTACGGATAAACAGCATAATGTTGACAATAATAAGTGGCAAAAT11660.11937132467362284No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11620.11896181755638913No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11540.11814280332192173No Hit
GTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATATGGGACAGACTTTACT11480.11752854264607118No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11470.11742616586676276No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11460.11732378908745433No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11420.11691428197022065No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11410.11681190519091222No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11320.1158905141771364No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11260.11527625350128586No Hit
GTTATAATAGTGCTTACAAATACTACGCAGACTCTGTGAAGGGCCGATTC11150.11415010892889318No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11140.11404773214958475No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11080.11343347147373421No Hit
ATAATACACCGCCGTGTCCTCGACTCTCAGGCTATTCATTTCCAGATACA10890.11148831266687416No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10810.11066929843240676No Hit
CCATCAACAGCCTGCAGACTGAAGATTTTGCCACTTATTATTGTCAACAT10740.10995266097724779No Hit
TCATAGATCAGCGACCTGGGGGCTTTCCCGGGTCTCTGCTGAAACCAGGC10730.10985028419793937No Hit
GATAAACAGCATAATGTTGACAATAATAAGTGGCAAAATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTAAAGT10690.10944077708070565No Hit
CGGATAAACAGCATAATGTTGACAATAATAAGTGGCAAAATCTTCAGTCT10670.10923602352208882No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10520.10770037183246244No Hit
GGTTTCAGCAGAGACCCGGGAAAGCCCCCAGGTCGCTGATCTATGATGCAAGTAGTTTGCAGAGTGGGGTCCCGT10480.10729086471522875No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10430.10677898081868664No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10380.1062670969221445No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAGCTGGGGCTCCGCTGGG10340.1058575898049108No Hit
GTTGATGGTGAGAGTAAAGTCTGTCCCATATCCACTGCCGCTGAACCTTG10300.10544808268767711No Hit
GAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATATGGGACAGACTTTACTCTCACCATCAACAGCCTGCAGA10260.10503857557044342No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10230.10473144523251814No Hit
GGACCCCACTCTGCAAACTACTTGCATCATAGATCAGCGACCTGGGGGCTTTCCCGGGTCTCTGCTGAAACCAGG10190.10432193811528445No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10130.1037076774394339No Hit
GAATATGGGGCCAGGGAACCCGGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGC10030.10268390964634966No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9970.10206964897049911No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9910.10145538829464855No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9870.10104588117741486No Hit
CTACTTGCATCATAGATCAGCGACCTGGGGGCTTTCCCGGGTCTCTGCTG9840.10073875083948958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG150.002519871667.8356128
TACGTAT300.003311663663.31236370
GCAACCC200.007944562550.7568743
CGTATGC605.513357E-946.0895141
GGTATCA8300.044.859211
ATCTCGT651.19252945E-842.23707637
TCGTATG702.1853339E-839.43516540
CCGTCTT801.4406396E-939.32825547
ATGCCGT801.5261321E-939.09867544
AGACCGA702.5787813E-838.7016325
TCTCCGA702.600973E-838.66398611
GTATCAA21650.038.6167831
GACCGAG754.7222784E-836.1326326
GCCGTCT806.412483E-834.8975346
TATCAAC24100.034.5434842
ATCAACG25900.032.2701383
GATCTCG851.281278E-732.2537236
AATGGTA3750.032.07826670
ACGAGAC951.0621079E-832.05697322
ATCTCCG851.4346551E-731.84092910
Produced by FastQC (version 0.11.5)