FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976784
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47480.48608494815639897No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35650.3649732182345329No Hit
GACTTTACTCTCACCATCAACAGCCTGCAGACTGAAGATTTTGCCACTTATTATTGTCAACATTATGCTGTTTAT35570.36415420400006554No Hit
GAGTAAAGTCTGTCCCATATCCACTGCCGCTGAACCTTGACGGGACCCCA34710.355349800979541No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31030.31767514619404086No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30970.31706088551819034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26720.27355075431210996No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26540.27170797228455834No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC25710.2632106996019591No Hit
ACATTATGCTGTTTATCCGTACACTTTTGGCCGGGGGACCAAGGTGGAAATCAGTCGAACTGTGGCTGCACCAT25030.2562490786089862No Hit
GTGCAGCCACAGTTCGACTGATTTCCACCTTGGTCCCCCGGCCAAAAGTG24030.2460114006781438No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23380.2393569100230962No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23170.2372069976576193No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22930.2347499549542171No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22790.2333166800438992No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21010.2150936133269996No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20740.21232944028567213No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19800.2027060230306803No Hit
GTCTCCAACCTCACTGTCTGCATTTGTAGGACAGACAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAAGAC19740.20209176235482973No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19560.2002489803272781No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19500.1996347196514275No Hit
GCATAATGTTGACAATAATAAGTGGCAAAATCTTCAGTCTGCAGGCTGTT18520.18960179527920196No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18260.18693999901718292No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18060.18489246343101445No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT17560.1797736244655932No Hit
CTTTTGGCCGGGGGACCAAGGTGGAAATCAGTCGAACTGTGGCTGCACCA17260.17670232108634049No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGACTGATTTCCACCTTGGTCC17180.1758833068518731No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17070.1747571622794804No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT17010.17414290160362986No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16930.17332388736916246No Hit
GGATATGGGACAGACTTTACTCTCACCATCAACAGCCTGCAGACTGAAGA16730.171276351782994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16130.16513374502448852No Hit
GTTTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCAACCTCA15890.16267670232108636No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGACAT15870.16247194876246948No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG15770.16144818096938524No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15760.16134580419007682No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCT15530.15899113826598305No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC15070.15428180641779554No Hit
ATTAAGACCTTTGTAGCCTGGTTTCAGCAGAGACCCGGGAAAGCCCCCAG14870.15223427083162705No Hit
ATATGGGACAGACTTTACTCTCACCATCAACAGCCTGCAGACTGAAGATT14840.15192714049370176No Hit
GTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATATGGGACAGACTTTACT14730.1508009959213091No Hit
GTCCCATATCCACTGCCGCTGAACCTTGACGGGACCCCACTCTGCAAACT14530.1487534603351406No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC14520.1486510835558322No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14350.146910678307589No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCAACCTCA14180.14517027305934577No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14010.14342986781110256No Hit
CACCATCAACAGCCTGCAGACTGAAGATTTTGCCACTTATTATTGTCAAC13900.1423037232387099No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13690.14015381087323298No Hit
GTTGATGGTGAGAGTAAAGTCTGTCCCATATCCACTGCCGCTGAACCTTG13390.13708250749398024No Hit
GGCCAAAAGTGTACGGATAAACAGCATAATGTTGACAATAATAAGTGGCA13160.1347278415698865No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12950.13257792920440958No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12870.1317589149699422No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12520.12817572769414734No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12510.1280733509148389No Hit
AGTCAGGATATTAAGACCTTTGTAGCCTGGTTTCAGCAGAGACCCGGGAA12490.12786859735622205No Hit
GGATATTAAGACCTTTGTAGCCTGGTTTCAGCAGAGACCCGGGAAAGCCC12460.12756146701829676No Hit
GTTTATCCGTACACTTTTGGCCGGGGGACCAAGGTGGAAATCAGTCGAACTGTGGCTGCACCATCTGTCTTCAT12270.1256163082114367No Hit
GGTCTTAATATCCTGACTCGCCCGACAAGTGATGGTGACTGTCTGTCCTACAAATGCAGACAGTGAGGTTGGAGA12170.12459254041835247No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12030.12315926550803453No Hit
GATCTATGATGCAAGTAGTTTGCAGAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATATGGGACAGACTTTA12030.12315926550803453No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12020.12305688872872611No Hit
CCCATATCCACTGCCGCTGAACCTTGACGGGACCCCACTCTGCAAACTAC12020.12305688872872611No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11920.12203312093564185No Hit
CAATAATACACCGCCGTGTCCTCGACTCTCAGGCTATTCATTTCCAGATA11900.12182836737702502No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11420.11691428197022065No Hit
GTTATAATAGTGCTTACAAATACTACGCAGACTCTGTGAAGGGCCGATTC11390.11660715163229536No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11260.11527625350128586No Hit
ACCTCACTGTCTGCATTTGTAGGACAGACAGTCACCATCACTTGTCGGGC11230.11496912316336057No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11190.11455961604612688No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11040.11302396435650053No Hit
GATTTCCACCTTGGTCCCCCGGCCAAAAGTGTACGGATAAACAGCATAATGTTGACAATAATAAGTGGCAAAAT10950.11210257334272469No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10720.10974790741863094No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10700.10954315386001408No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10700.10954315386001408No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10590.10841700928762144No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10490.10739324149453718No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10420.1066766040393782No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10370.10616472014283607No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10270.10514095234975185No Hit
CGGATAAACAGCATAATGTTGACAATAATAAGTGGCAAAATCTTCAGTCT10140.10381005421874231No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10070.10309341676358334No Hit
GAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATATGGGACAGACTTTACTCTCACCATCAACAGCCTGCAGA10050.10288866320496651No Hit
ATAATACACCGCCGTGTCCTCGACTCTCAGGCTATTCATTTCCAGATACA9960.10196727219119069No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9900.10135301151534015No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9890.10125063473603171No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAGCTGGGGCTCCGCTGGG9770.10002211338433062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)