FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925063
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATACTTACCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT112730.5855912248066687No Hit
GTATCATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATGGTGAGAGTGAAATCTGTC110040.571617656149435No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70500.3662217807936675No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62500.3246646992851663No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA59710.3101716671090764No Hit
GGCTATTACCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGACAAAGCCCCTAAGTCCCTGATCTATGATACGTC50610.26290048689315626No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48810.2535501435537434No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTC44770.2325638173919503No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43860.22783669937035825No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43720.2271094504439595No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43190.2243562937940213No Hit
ATATATTACTGTGCGAAAGATTGGAGCACCGGTGTCCCCCTTTACTACTT40790.2118891693414709No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA40530.21053856419244463No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36930.19183787751361903No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36700.19064311142024962No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35760.18576015434300072No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35650.18518874447225883No Hit
GCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT35520.18451344189774568No Hit
GTCTCCATCCTCACTGTCTGCGTCTGTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTACCAG33840.17578645478096042No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33280.17287745907536534No Hit
CAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT32950.17116322946313967No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32540.16903342903582896No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32310.16783866294245953No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31790.16513745264440696No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31460.1634232230321813No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30910.16056617367847184No Hit
GGCCAAAAGTGTTCGGGTAAGTATCATACTGTTGGCAGTAATAAGTTGCA30640.1591636221775599No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30380.1578130170285336No Hit
GTAATAGCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCGACAGA30130.15651435823139295No Hit
GGTAAGTATCATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATGGTGAGAGTGAAAT29460.15303395265505595No Hit
GTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTACCAGCTGGTTAGCCTGGTATCAGCAGAAA29130.15131972304283028No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG28190.1464367659655814No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28170.14633287326181013No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27830.14456669729769883No Hit
ATACTTACCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA27110.14082655996193372No Hit
GGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCT27000.14025515009119183No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA26720.13880065223839427No Hit
GTCTGCGTCTGTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG26270.13646306640354108No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25780.13391769516114538No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGG25620.13308655353097534No Hit
GATCTATGATACGTCCAAGTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTAGATCTGGGACAGATTTCA25610.13303460717908971No Hit
GATTTCACTCTCACCATCAACAACCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATGATACTTAC25340.1316320556781778No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCGCTGAACCTTGATGGGACCCCA25220.1310086994555503No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24740.1285152745650402No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24460.12706077671224267No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24380.12664520589715764No Hit
GTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCC24160.12550238615567386No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23700.12311285396893505No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23700.12311285396893505No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23160.1203077509671112No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22980.11937271663316992No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22730.11807405783602926No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA22440.1165676136313461No Hit
GTCCCTGATCTATGATACGTCCAAGTTGGAAAGTGGGGTCCCATCAAGGT22340.11604815011248981No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22260.11563257929740481No Hit
ACCTTGATGGGACCCCACTTTCCAACTTGGACGTATCATAGATCAGGGACTTAGGGGCTTTGTCTGGTTTCTG21890.11371056427763662No Hit
TCATAGATCAGGGACTTAGGGGCTTTGTCTGGTTTCTGCTGATACCAGGC21850.11350277887009412No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21710.11277552994369536No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21630.11235995912861033No Hit
CAGTATGATACTTACCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21490.11163271020221156No Hit
GTCTTGTGTTTCCTCTGGATTCACATTTGACTCCAATGGCATGAGTTGGGTCCGCCTGGCTCCAGGGAAGGGG21370.11100935397958406No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20780.10794451921833208No Hit
TCCTCACTGTCTGCGTCTGTCGGAGACAGAGTCACCATCACTTGTCGGGC20570.10685364582873393No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20430.10612639690233514No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA20100.10441216729010948No Hit
GCCCCTAAGTCCCTGATCTATGATACGTCCAAGTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTAGAT20080.10430827458633822No Hit
CATTATATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCATATAT20000.10389270377125319No Hit
GATCAGGGACTTAGGGGCTTTGTCTGGTTTCTGCTGATACCAGGCTAACC19470.10113954712131498No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19260.10004867373171683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA200.007164473652.1031645
CTAGCGC200.007682761651.1908337
CGTATGC2150.048.07636341
TCGTATG2150.047.9438140
ACGAGAC2650.041.94144422
CGAGACG2700.041.16903323
ATCTCGT2600.040.69014437
TCTGGAG19950.040.0737370
GTATCAA35800.039.32091
ATGCCGT2750.039.0788144
AGACGGA2950.037.69874625
ATCTCCG2950.037.65762310
GTGGTAT3950.037.5133441
CCGTCTT2900.037.32404347
CCCACGA3000.037.03769319
GTATGCC2900.036.9123642
GCCGTCT3050.036.5495346
CACGAGA3000.035.92280221
TATACAC3500.035.591893
GGAGCTA3100.034.88092429
Produced by FastQC (version 0.11.5)