FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925063
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATGGTGAGAGTGAAATCTGTC109910.570942353574922No Hit
GTATGATACTTACCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT106030.5507871690432988No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70150.3644036584776706No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55160.28653607700111633No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA48640.2526670555716878No Hit
GGCTATTACCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGACAAAGCCCCTAAGTCCCTGATCTATGATACGTC48170.2502255770330633No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47460.24653738604918382No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47460.24653738604918382No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40690.21136970582261463No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40290.20929185174718956No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTC39900.20726594402365014No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA39400.20466862642936878No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38960.20238298694640125No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37060.19251318008813217No Hit
ATATATTACTGTGCGAAAGATTGGAGCACCGGTGTCCCCCTTTACTACTT36520.18970807708630835No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33870.1759422938366173No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33540.1742280642243916No Hit
GTCTCCATCCTCACTGTCTGCGTCTGTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTACCAG32960.17121517581502527No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32410.16835812646131582No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32100.16674778955286138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31930.16586470157080574No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31470.16347516938406692No Hit
GCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT31330.16274792045766814No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG31200.162072617883155No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30970.1608778517897856No Hit
GGCCAAAAGTGTTCGGGTAAGTATCATACTGTTGGCAGTAATAAGTTGCA30570.15879999771436054No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30450.158176641491733No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30400.15791690973230485No Hit
CAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT29990.15578710930499418No Hit
GTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTACCAGCTGGTTAGCCTGGTATCAGCAGAAA28690.14903408355986272No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28610.1486185127447777No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27810.14446280459392757No Hit
GTAATAGCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCGACAGA27770.14425501918638506No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCGCTGAACCTTGATGGGACCCCA27550.14311219944490128No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26760.1390084376459368No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26170.13594360288468482No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26160.1358916565327992No Hit
GATTTCACTCTCACCATCAACAACCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATGATACTTAC26150.13583971018091356No Hit
GGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCT25800.13402158786491664No Hit
GGTAAGTATCATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATGGTGAGAGTGAAAT25780.13391769516114538No Hit
ATACTTACCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA25570.13282682177154723No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA25340.1316320556781778No Hit
GATCTATGATACGTCCAAGTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTAGATCTGGGACAGATTTCA25190.1308528603998934No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGG25190.1308528603998934No Hit
GTCTGCGTCTGTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG24900.12934641619521023No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24810.1288788990282396No Hit
GTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCC23880.12404788830287632No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC23310.12108694624539561No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23210.12056748272653935No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23100.11999607285579746No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22190.11526895483420542No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22010.11433392050026416No Hit
GTCTTGTGTTTCCTCTGGATTCACATTTGACTCCAATGGCATGAGTTGGGTCCGCCTGGCTCCAGGGAAGGGG21670.11256774453615286No Hit
GTCCCTGATCTATGATACGTCCAAGTTGGAAAGTGGGGTCCCATCAAGGT21410.11121713938712656No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA21330.11080156857204153No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21060.10939901707112962No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21030.10924317801547273No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20740.10773673381078958No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20720.10763284110701832No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20570.10685364582873393No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20130.10456800634576634No Hit
CAGTATGATACTTACCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20010.10394465012313883No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19760.10264599132599816No Hit
GCCCCTAAGTCCCTGATCTATGATACGTCCAAGTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTAGAT19690.10228236686279878No Hit
ACCTTGATGGGACCCCACTTTCCAACTTGGACGTATCATAGATCAGGGACTTAGGGGCTTTGTCTGGTTTCTG19620.10191874239959939No Hit
TCATAGATCAGGGACTTAGGGGCTTTGTCTGGTTTCTGCTGATACCAGGC19500.10129538617697188No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19460.10108760076942937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)