FastQCFastQC Report
Wed 8 May 2024
EGAF00004691985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205697
Total Bases89.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62270.5164647502647846No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT60410.5010379888147686No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT42290.3507514740436445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40510.3359882292151345No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40080.3324218273745394No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39350.3263672382032965No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC38700.32097616565355974No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38030.3154192139484464No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG37060.30737407491268537No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35420.293771984171811No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT34720.2879662137336329No Hit
TCCTTAGCCTGGTATCAACACAAACCTGGCCAGGCTCCCAGGCTCGTCAT34090.2827410203392727No Hit
GCCTAGAGCCTGAGGATTCTGCAATTTATTACTGTCAGCAGCGTGAGCAC33690.2794234372317423No Hit
GTAATAGACAGCCGTGTCTTCGGGTCGCAGGCTGCCCATTTGCAAATATA33510.2779305248333537No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33110.2746129417258233No Hit
CTACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCGGTGGGTCTGGGA31950.26499195071398535No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA30000.24881873306477498No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC29780.2469940623556333No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCT29590.24541821037955638No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28960.2401930169851961No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28330.23496782359083584No Hit
GTCCCAGACCCACCGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGTA27530.22833265737577518No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA26370.21871166636393718No Hit
CTCCTGCAGGGCCAGTCAGGATGTTCGCACCTCCTTAGCCTGGTATCAAC25980.21547702283409514No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC25970.21539408325640688No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25210.20909067535209927No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24730.20510957562306287No Hit
GTCTATTACTGTGCGAAAGCCGGGGGTGAATACAATGAGTACGCCACTCT24570.2037825423800507No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGG23660.19623504081041923No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTCTGCAATTTA23500.19490800756740706No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATGCATCCTACA23350.1936639139020832No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22650.18785814346390511No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21650.1795641856950793No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20520.17019201341630608No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC20390.16911379890635872No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT20350.1687820405956057No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG19650.16297627015742763No Hit
GGCTAAGGAGGTGCGAACATCCTGACTGGCCCTGCAGGAGAGGGTGGCTC19190.15916104958376773No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18300.15177942716951273No Hit
GTTCAGTGGCGGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC18190.1508670918149419No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18100.15012063561574757No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17740.14713481081897029No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17670.14655423377515248No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16980.14083140291466265No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16870.1399190675600918No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGAACCTGCCTGGGATGCCA16720.1386749738947679No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16690.13842615516170315No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16680.13834321558401488No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16670.13826027600632665No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG16610.13776263854019707No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC16590.13759675938482058No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16570.13743088022944405No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16290.13510857205417282No Hit
GTCAGGATGTTCGCACCTCCTTAGCCTGGTATCAACACAAACCTGGCCAG15980.13253744514583682No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC15970.13245450556814856No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15870.13162510979126596No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15870.13162510979126596No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT15720.13038101612594208No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15690.13013219739287732No Hit
GCGTGAGCACTGGTTGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA15680.13004925781518906No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15660.12988337865981256No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA15580.12921986203830646No Hit
GCCCTGTAGGATGCATCATAGATGACGAGCCTGGGAGCCTGGCCAGGTTT15450.12814164752835913No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15410.12780988921760608No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15290.126814614285347No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGC15190.1259852185084644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.12440936653238749No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT14830.12299939371168711No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14620.12125766258023367No Hit
GTATCAACACAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATGCAT14590.12100884384716891No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14580.12092590426948063No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14520.1204282668033511No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC14300.11860359609420941No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14300.11860359609420941No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14060.11661304622969121No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13720.11379310058829042No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA13330.11055845705844834No Hit
CCACTGAACCTGCCTGGGATGCCAGTGGCCCTGTAGGATGCATCATAGAT13210.10956318212618926No Hit
GGTTTGTGTTGATACCAGGCTAAGGAGGTGCGAACATCCTGACTGGCCCT13170.10923142381543621No Hit
ATTTATTACTGTCAGCAGCGTGAGCACTGGTTGCTCACTTTCGGCGGAGG13110.10873378634930667No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC13100.1086508467716184No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13080.10848496761624189No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12990.10773851141704757No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA12960.10748969268398278No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12900.10699205521785324No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12810.1062455990186589No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12740.1056650219748411No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCC12670.10508444493102329No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA12630.10475268662027026No Hit
GTGCTCACGCTGCTGACAGTAATAAATTGCAGAATCCTCAGGCTCTAGGC12540.10400623042107594No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT12530.1039232908433877No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC12290.1019327409788695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)